Prokaryotes Flashcards
What was the first cellular life?
Cyanobacteria
Define Anaerobic
Without oxygen
All cells are derived from…
Prokaryotes
Prokaryotes have great ____ and high ____
Diversity; Survivability
Two of the three branches in the Tree of Life are
Prokaryotic
The “Prokaryote” branch in the Tree of Life includes:
Archaea and Bacteria
Last common ancestor of all life are the
Prokaryotes
_____ are essential for life on earth
Prokaryotes
Proteobacteria are gram _____
Negative
Prokaryotes lack a
Cell membrane
Two types of bacterial Cell Walls are
Gram Positive and Gram Negative
Gram positive/negative bacteria are determined by:
Cell wall
Three Functions of the Cell Wall are:
-Maintain cell shape
-provide physical protection
-prevent osmotic bursting
The Cell Wall contains
Peptidoglycan
Gram positive have a peptidoglycan that is
Tight and linked
Where is Peptidoglycan found
Cell wall of Bacteria
Gram negative bacteria have a peptidoglycan structure that is
Loose and thin
Which gram bacteria has an extra membrane
Gram negative
Which gram bacteria stains purple
Gram positive
Which gram bacteria stains pink/red
Gram negative
Gram negative bacteria will stain
Pink/Red
Gram positive bacteria will stain
Purple/Blue
What is Lipopolysaccharide?
Endotoxin found on cell wall of gram negative bacteria
What are the 6 functions of the Cell/Plasma Membrane?
- Regulation of movement into and out of cell
- Synthesizes cell wall components
- Assists with DNA replication
- Secretes proteins
- Contains the base of appendages (ie. flagella)
- Can respond to environment
What does ‘selectively permeable’ mean?
Regulation of movement of material into and out of the cell by transport mechanism
What are the structural components of the cell/plasma membrane?
Hydrophilic end/head (phosphate group) and a hydrophobic centre (fatty acid molecules)
What’s the Lipid Bilayer?
The central, hydrophobic portion of the Cell/Plasma Membrane
The Capsule is made of?
Sugars and/or Proteins
What part of bacteria is used as protection against host immune system (ie. phagocytosis)?
The Capsule
What structure is external to the cell wall in bacteria?
Capsule
What are three functions of the Capsule?
- Adhesion to substrate
- Cohesion/Biofilms (ie. plaque)
- Protection against host immune system (phagocytosis)
Pili, Fimbriae, and Flagellum are?
Surface filaments
What are three types of surface filaments?
- Pili & Fimbriae
- Flagellum
Leptospirosis contains an ______ flagella.
Internal
Four types of morphology (structure) of bacteria?
- Bacillus (rods)
- Coccus (balls)
- Coccobacillus (grape bundle)
- Spirochaete (squiggly lines)
What are the 7 main structural components of bacteria?
- Cell Wall
- Cell/Plasma Membrane
- Capsule
- Surface Filaments (Pili & Fimbriae, Flagellum)
- Nucleoid
- Ribosomal DNA in Cytoplasm
- DNA on Plasmids
The complete set of genetic material within a cell is called a?
Genome
A Haploid is?
A single ring of DNA
The Nucleoid in prokaryotic cells contains?
Genetic material
Prokaryotic cytoplasm contains
Ribosomal DNA
DNA of prokaryotes is located in the
Nucleoid
rDNA is located in the
Cytoplasm
Multi-drug resistance genes are located in
DNA on Plasmids
Plasmids are _______ for survival
NOT essential
Large Gram+ rods are called
Endospores
Give an example of an Endospore
Bacillus anthracis (Anthrax), Clostridum tetani (Tetanus)
Anthrax and Tetanus are examples of
Large gram positive rods (Endospores)
Endospores are
Large gram + rods that are difficult to kill
What does “Thermostable” mean
Destroyed with certainty ONLY by moist heat at 121 C for 15 minutes
How can one destroy thermostable Endospores?
Autoclave
List three types of unusual bacteria:
- Mycoplasma
- Chlamydia
- Rickettsia
What’s a vector-borne disease?
Transmitted via fleas, ticks, mites, etc.
What bacteria LACKS a cell wall
Mycoplasma
What is a mixed community of bacteria called?
Biofilms
Where are biofilms located?
Embedded in a matrix
What is the mode of nutrition for prokaryotes called?
Chemoheterotroph
Define Chemoheterotroph
Mode of nutrition for many prokaryotes - energy is obtained via organic compounds
What are Obligate Aerobes
Require oxygen for survival
What are Facultative Anaerobes
Can survive with or without oxygen
What are Obligate Anaerobes?
Will be poisoned by oxygen
How do Obligate Anaerobes survive?
Use inorganic molecules or fermentation (organic molecules)
What are the four types of metabolic relationships with oxygen
- Obligate Aerobes
- Obligate Anaerobes
- Facultative Anaerobes
- Aerotolerant Anaerobes
Generation/Doubling time means
The time it takes for a cell to divide and the population to double
Bacterial reproduction is done by
Asexual Binary Fission
Asexual Binary Fission does NOT require
-meiosis
-fertilization
Genetic variation can occur through four methods:
Mutation, Transformation, Conjugation, Transduction
Transduction occurs by
Gene transfer between cells by viruses
Conjugation occurs by
Plasmid gene transfer
Transformation occurs by
Foreign DNA from the environment
Major source of genetic variation occurs by
Mutation
Bacteria have high ____ and ____
Diversity, Survivability
Six types of Extremophiles:
Thermophile (high temperature), Psychrophile (low temperature), Acidophile (acid), Barophile (pressure), Xerophile (dry), Halophile (salt)
Give an example of human applications of Prokaryotes
Microbial Leaching/Mining, Fuel Production, Bioremediation (ie. sewage, water purification, herbicides, oil spills, etc.), Antimicrobials, vitamins, vaccines, insulin, etc., Detergents, Food products (vinegar, citric acid, etc.), Genetic engineering
Types of Symbiotic Associations:
Commensalism, Mutualism, Parasitic/Pathogen
Term for symbiotic association where one organism benefits and one organism neither benefits nor is harmed
Commensalism
An example of a Commensalism Symbiotic Association is
Staphylococcus
A symbiotic association where two organisms of different species benefit from association
Mutualism
What’s an example of a Mutualistic Symbiotic Association
E.coli
What types of parasitic/pathogen associations are there
Opportunistic Pathogens, Obligate Pathogens
What is it called when a pathogen causes disease when it enters a host?
Opportunistic Pathogen
What’s it called when a pathogen requires a host to fulfill its life cycle?
Obligate Pathogen
A microorganism that is able to produce disease is called
A Pathogen
Indigenous Microbiota are
Acquired soon after birth, are host-specific and organ-specific
Examples of Indigenous Microbiota:
B.bifidum (human GI), Staphylococcus epidermidis (human skin), Staphylococcus pseudintermedius (dog skin), E.coli (all species, intestine), Pasteurella multocida (upper respiratory tract of most animals)
What can contribute to commensals causing infection
Lowered host defense mechanisms (ie. immunosuppressed, diabetic), Normal flora disturbed (ie. oral antibiotics), Change in natural habitat of the organism
Example of a commensal turned into opportunistic infection
Fusobacterium necrophorum
How is acidosis of the rumen caused?
Too much grain in feed —> fusobacterium necrophorum
What is an example of an Anaerobic Gram Negative Bacteria that affects cattle?
Fusobacterium necrophorum
Define Opportunistic Pathogen
A microorganism that is normally a commensal in the host’s body, but can cause disease when it gains access to an abnormal location or it infects a host with a diminished immune system
Give an example of a Uropathogenic Opportunistic Pathogen
E.coli
Three functions of the intestinal microbiome
Protection, Structure, Metabolism
What’s an example of mutualism
Gut microbiome
What is the rumen
A large, pre-gastric fermentation chamber found in ruminants
What is the primary energy source in ruminants
Volatile Fatty Acids (VFA)
What is a VFA
Volatile Fatty Acid —> byproduct of microbes within the rumen
What weighs 10-20% of total body weight in ruminants?
The Rumen
List the 5 major groups of rumen microorganisms:
Bacteria, Protozoa, Fungi, Archaea, Bacteriophages
What are some negative interactions within the rumen
Predation, pathogens, competition for space and resources
Functions of Escherichia coli
Synthesizes vitamin K in intestine, essential for blood clotting, breakdown and absorb food, waste processing
Disease definition
Injury to a host that impairs the function of host tissue
Infection definition
Growth of microorganisms in/on tissues of the host
Inflammation definition
Response of host tissue to injury or infection. Infiltration of tissue with WBCs leading to redness, swelling, and pain
Invasiveness
Ability of a microorganism to enter the body and spread
Pathogen
An organism that causes harm to the host
Pathogenicity
The capacity of bacterium to cause disease
Virulence
The degree or severity of disease caused by the infectious agent
Pathogenesis
Mechanism of infection (ie. how the disease develops)
Adhesions
Allows attachment to the host (ie. Fimbriae, Pili, Surface Proteins)
List the 5 types of Exotoxins
Cytotoxins, Haemolysins, Proteases, Phospholipases, Leukocidins
Example of an Exotoxin
Botulism, Cholera
Examples of an Endotoxin
LPS, Pyrogenic, Toxic Shock
What are two types of toxins
Exotoxins, Endotoxins (gram-negative bacteria only)
What is the function of exotoxins
Produce enzymes to help bacteria travel through host
Flagella can be used for
Motility, immune evasion
What part of bacteria is antiphagocytic
Capsule
What can enterotoxigenic E.coli cause
Hypersecretory diarrhoea resulting in rapid dehydration and acidosis
Enterohaemorrhagic E.coli affects ____ and is mainly carried by ____
Humans, cows
Enterohaemorrhagic E.coli can cause
Kidney failure, oedema in brain
Anthrax is usually associated with which specie(s)
Sheep, goats, and cattle (occasionally pigs and humans)
How is Bacillus anthracis (Anthrax) obtained
Ingestion of Endospore
What does AMR stand for
Antimicrobial Resistance
Antimicrobial Resistance (AMR)
Microorganism formally susceptible to the action of the antimicrobial is no longer affected by it. Ie. Bacteria has changed so the antibiotic no longer works
What are two types of AMR
Intrinsic Resistance, Acquired Resistance
Intrinsic Resistance
Structural or functional characteristic in the microorganism that provides AMR
Acquired Resistance
Genetic mutation or acquisition of genetic element (plasmids) that provide microorganism with AMR
What are two eradicated diseases
Rinderpest, Small pox
Nosocomial Infection
A pathogen obtained in hospital. Ex. UTI, MRSA, SSI
One organism benefits, one organism neither benefits nor is harmed
Commensalism
Staphylococcus is a type of _______ symbiotic association
Commensalism
Both organisms benefit from association
Mutualism
E.coli is an example of a _____ symbiotic relationship
Mutualistic
One organism benefits while the host is disrupted
Parasitic/Pathogenic
Two types of Parasitic/Pathogenic relationships are:
Opportunistic, Obligate
Anthrax is an example of a ______ symbiotic association
Parasitic/Pathogenic
A pathogen that causes disease when it enters a host is called
Opportunistic Pathogen
A pathogen that requires a host to fulfill its life cycle is called
Obligate Pathogen
Indigenous Microbiota are an example of
Commensals
Commensals can cause Opportunistic Infections when
Host defense mechanism is lowered (immunosuppressed, diabetic), Normal flora is disturbed (oral antibiotics), Change in natural habitat of the commensal (ex. Fusobacterium necrophorum)
Fusobacterium necrophorum
Anaerobic Gram-negative Bacteria, Commensal turned into Opportunistic when too much grain is given (results in acidosis of the Rumen)
Opportunistic Pathogen
A microorganism that is normally a commensal in the host’s body, but can cause disease when it gains access to an abnormal location or infects a host with a diminished immune status
Three functions of intestinal microbiome
Protection, Structural, Metabolic
Ruminants evolved
A large pre-gastric fermentation chamber (the Rumen)
Uropathogenic E.coli is an example of
Commensal turned Opportunistic Infection
5 Major Groups of Rumen Microorganisms are:
Bacteria (break down plant material into VFA), Protozoa (consume and ferment bacteria into VFA), Fungi (assist in fibre degradation), Archaea (produce methane), Bacteriophages (viruses that attack bacteria)
VFA is
Volatile Fatty Acid (primary source of energy in ruminants)
Negative Interactions of the rumen include
Predation, Pathogens, Competition for space and resources
Example of a Synergistic Interaction (cross feeding)
Cellulolytic bacteria
4 Functions of Escherichia coli include
Synthesis of vitamin K in intestine, blood clotting, breakdown of food, food absorption and waste processing
Injury to the host that impairs the function of host tissue
Disease
Growth of microorganisms in/on tissues of the host
Infection
The response of the host tissue to injury or infection (ie. infiltration of tissue with WBCs, redness, swelling, pain)
Inflammation
Ability of a microorganism to enter the body and spread
Invasiveness
An organism that causes harm to the host
Pathogen
The degree/severity of a disease
Virulence
Mechanism of infection/by which the disease develops
Pathogenesis
Adhesins
Allows attachment to the host (ex. Fimbriae, pili, surface proteins)
Two types of toxins include
Exotoxins, Endotoxins
What is the purpose of Exotoxins
Produce enzymes to help bacteria travel
2 Examples of pathogens with exotoxins include
Botulism, Cholera
5 types of Exotoxins
Cytotoxins, Haemolysins, Proteases, Phospholipases, Leukocidins
Examples of Endotoxins include
LPS, pyrogenic, toxic shock
Clostridium botulinum (Botulism) release the neurotoxin Botulinum toxin, which prevents
Release of acetylcholine from synapse, resulting in paralysis and death