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Mycology PPT > PRIMARY RECOVERY MEDIA AND DIFFERENTIAL MEDIA > Flashcards

PRIMARY RECOVERY MEDIA AND DIFFERENTIAL MEDIA Flashcards

1
Q

Primary recovery of saprobic and pathogenic fungi

A

Brain-heart infusion agar

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2
Q

Brain-heart infusion agar composition

A

Brain-heart infusion, enzymatic digest of animal tissue, enzymatic digest of casein, dextrose, sodium chloride

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3
Q

Brain-heart infusion agar with antibiotics composition

A

Brain-heart infusion, enzymatic digest of animal tissue, enzymatic digest of casein, dextrose, sodium chloride, antibiotics

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4
Q

Recovery of fungi from blood

A

Brain-heart infusion biphasic blood culture bottles

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5
Q

Brain-heart infusion biphasic blood culture bottles composition

A

Brain-heart infusion, peptone, glucose, disodium phosphate

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6
Q

Isolation and presumptive identification of yeast and filamentous fungi

A

Chromogenic agar

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7
Q

Chromogenic agar composition

A

Chromopeptone Glucose Chromogen mix Chloramphenicol

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8
Q

Primary recovery of dermatophytes; recommended as screening medium

A

Dermatophyte test medium

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9
Q

Dermatophyte test medium composition

A

Dextrose, cycloheximide, gentamycin, chloramphenicol, phenol red

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10
Q

Primary recovery of pathogenic fungi exclusive of dermatophytes

A

Inhibitory mold agar

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11
Q

Inhibitory mold agar composition

A

Chloramphenicol, casein, dextrose, starch, sodium phosphate, magnesium sulfate, sodium chloride, manganese sulfate

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12
Q

Potato flake agar composition

A

Potato flakes, glucose, cycloheximide, chloramphenicol, bromthymol blue

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13
Q

Primary recovery of dermatophytes

A

Mycosel agar

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14
Q

Mycosel agar composition

A

Cycloheximide, chloramphenicol, dextrose

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15
Q

Primary recovery of saprobic and pathogenic fungi

A

Sabouraud Dextrose with Brain Heart Infusion (SABHI) agar

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16
Q

Sabouraud Dextrose with Brain Heart Infusion (SABHI) agar composition

A

Sabouraud dextrose, brain-heart infusion agar

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17
Q

Primary recovery of pathogenic fungi exclusive of dermatophytes

A

Yeast-extract phosphate agar

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18
Q

Yeast-extract phosphate agar composition

A

Yeast extract, dipotassium phosphate, chloramphenicol

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19
Q

Detection of ascospores in ascosporogenous yeasts

A

Acetate Ascospore agar

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20
Q

Acetate Ascospore agar composition

A

Potassium acetate, yeast extract, dextrose

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21
Q

Identification of
Cryptococcus, Trichosporon, and Rhodotorula spp

A

Christensen’s urea agar

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22
Q

Christensen’s urea agar composition

A

2% urea, phenol red

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23
Q

Identification of Candida albicans by chlamydospore production Identification of C. albicans by microscopic morphology

A

Cornmeal agar with Tween 80 and trypan blue

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24
Q

Cornmeal agar with Tween 80 and trypan blue composition

A

Cornmeal, Tween 80, trypan blue

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25
Q

Conversion of the dimorphic fungus Blastomyces spp. from mold to yeast form

A

Cottonseed conversion agar

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26
Q

Cottonseed conversion agar composition

A

Cottonseed meal, glucose

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27
Q

Differential identification of Aspergillus spp.

A

Czapek’s agar

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28
Q

Czapek’s agar composition

A

Sodium nitrate, sucrose, yeast extract

29
Q

Identification of
Cryptococcus neoformans and Cryptococcus gattii

A

Niger seed agar (birdseed agar)

30
Q

Niger seed agar (birdseed agar) composition

A

Guizotia abyssinica seed, dextrose, chloramphenicol

31
Q

Detection of nitrate reduction to confirm Cryptococcus spp.

A

Nitrate reduction medium

32
Q

Nitrate reduction medium composition

A

Potassium nitrate, peptone, meal extract, sulfanilic acid, N, N-dimethyl-1-naphthylamine

33
Q

Demonstration of pigment production by Trichophyton rubrum Preparation of microslide cultures and sporulation of dermatophytes

A

Potato dextrose agar

34
Q

Potato dextrose agar composition

A

Potato infusion, D(+) glucose

35
Q

Identification of
Microsporum audouinii

A

Rice medium

36
Q

Rice medium composition

A

White rice extract, polysorbate 80

37
Q

Identification of Trichophyton spp.

A

Trichophyton agars 1-7

38
Q

Trichophyton agars 1-7 composition

A

Casamino acids, dextrose, monopotassium phosphate, magnesium sulfate, amino acids, ammonium nitrate

39
Q

Detection of Cryptococcus spp. Differentiate Trichophyton mentagrophytes from Trichophyton rubrum Detection of Trichosporon spp,

A

Urea agar

40
Q

Urea agar composition

A

Peptone, dextrose, sodium chloride, monopotassium phosphate, urea, phenol red

41
Q

Identification of yeasts by determining fermentation

A

Yeast fermentation broth

42
Q

Yeast fermentation broth composition

A

Yeast extract, peptone, bromcresol purple, and a specific carbohydrate

43
Q

Identification of yeasts by determining carbohydrate assimilation

A

Yeast nitrogen-base agar

44
Q

Yeast nitrogen-base agar composition

A

Ammonium sulfate, carbon source

45
Q

Primary recovery media

A

Brain-heart infusion agar
Brain-heart infusion agar with antibiotics
Brain-heart infusion biphasic blood culture bottles
Chromogenic agar
Dermatophyte test medium
Inhibitory mold agar
Potato flake agar
Mycosel agar
Sabouraud dextrose woth brain heart infusion agar
Yeast-extract phosphate agar

46
Q

Primary recovery of saprobic and pathogenic fungi

A

BHI agar
Potato flake agar
Sabouraud Dextrose with Brain Heart Infusion (SABHI) agar

47
Q

Primary recovery of pathogenic fungi exclusive of dermatophytes

A

Yeast-extract phosphate agar
Inhibitory mold agar
Brain-heart infusion agar with antibiotics

48
Q

Primary recovery of dermatophytes

A

Dermatophyte test medium

Mycosel agar

49
Q

DIFFERENTIAL TEST MEDIA

A

Acetate Ascospore agar
Christensen’s urea agar
Cornmeal agar with Tween 80 and trypan blue Cottonseed conversion agar
Czapek’s agar
Niger seed agar (birdseed agar)
Nitrate reduction medium Potato dextrose agar Trichophyton agars
1-Urea agar
Yeast fermentation broth
Yeast nitrogen-base agar

50
Q

The agar provides a rich medium for bacteria, yeast, and pathogenic fungi.

A

Brain-heart infusion agar

51
Q

The agar provides a rich medium for yeast and pathogenic fungi, including systemic dimorphic fungi.

A

Brain-heart infusion agar (fungal formulation) with antibiotics

52
Q

Chromogen mix contains substrates that react with enzymes produced by differ- ent organisms that result in the production of character- istic color changes

A

Chromogenic agar

53
Q

Dermatophytes produce alkaline metabolites, which raise the pH and change the medium from red to yellow.

A

Dermatophyte test medium

54
Q

Examine plates for growth. Chloramphenicol inhibits bacterial growth.

A

Inhibitory mold agar

55
Q

Growth is enhanced by a pH alkaline reaction of fungus. Chloramphenicol and anti- biotics inhibit the growth of bacteria and nonpathogenic fungi.

A

Potato flake agar

56
Q

Inhibits bacteria and saprophytic fung

A

Mycobiotic or mycosel agar

57
Q

Isolates and enhances growth of all fungi including the yeast phase of dimorphic fungi.

A

Sabouraud dextrose with brain-heart infusion (SABHI) agar

58
Q

Enhances the recovery and sporulation of Blastomyces and Histoplasma capsu- latum from contaminated specimens

A

Yeast-extract phosphate agar with ammonia

59
Q

Potassium acetate is neces- sary, and yeast extract increases the sporulation of yeasts.

A

Acetate Ascospore agar

60
Q

Produces urease and a change in the pH.

A

Christensen’s urea agar

61
Q

enhances the production of chlamydospores, pseudohy- phal and arthrospore forma- tion. The addition of trypan blue provides a contrasting background for observing the morphologic features of yeasts.

A

Cornmeal agar with Tween 80 and trypan blue

62
Q

Produces characteristic fea- tures of yeast and fungus of any organism that can use sodium nitrate.

A

Czapek’s agar

63
Q

C. neoformans and C. gattii produce the enzyme phenol oxidase, resulting in a brown pigment through metabolism of caffeic acid

A

Niger seed agar (birdseed agar)

64
Q

Creatinine enhances the mela- nization of some strains of C. neoformans

A

Niger seed agar (birdseed agar)

65
Q

Carbohydrate and potato infu- sion promotes the growth of yeasts and molds, and the low pH (tartaric acid) partially inhibits bacterial growth.

A

Potato dextrose agar

66
Q

may be dif- ferentiated by growth in the presence of different amino acids.

A

Trichophyton agars 1–7

67
Q

Most yeasts produce acid, which is indicated by a change in the solution from purple to yellow as a positive fermenter.

A

Yeast fermentation broth

68
Q

Assimilation of carbon by yeast cells produces a positive result

A

Yeast nitrogen-base agar

Mycology PPT (7 decks)

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