Differential Media

Question 1

Detection of ascospores in ascosporogenous yeasts

Answer 1

Acetate Ascospore agar

Question 2

Identification of Cryptococcus, Trichosporon, and Rhodotorula spp.

Answer 2

Christensen’s urea agar

Question 3

Identification of Candida albicans by chlamydospore production

Answer 3

Cornmeal agar with Tween 80 and trypan blue

Question 4

Identification of C. albicans by microscopic morphology

Answer 4

Cornmeal agar with Tween 80 and trypan
blue

Question 5

Conversion of the dimorphic fungus Blastomyces spp. from mold to yeast form

Answer 5

Cottonseed conversion agar

Question 6

Differential identification of Aspergillus spp

Answer 6

Czapeks agar

Question 7

Identification of Cryptococcus neoformans and Cryptococcus gattii

Answer 7

Niger seed agar (birdseed agar)

Question 8

Detection of nitrate reduction to confirm Cryptococcus spp.

Answer 8

Nitrate reduction medium

Question 9

Demonstration of pigment production by Trichophyton rubrum Preparation of microslide cultures and sporulation of
dermatophytes

Answer 9

Potato dextrose agar

Question 10

Identification. of Microsporum audouinii

Answer 10

Rice medium

Question 11

Identification of Trichophyton spp

Answer 11

Trichophyton agars 1-7

Question 12

Detection of Cryptococcus spp.

Answer 12

Urea agar

Question 13

Differentiate Trichophyton mentagrophytes from Trichophyton rubrum

Answer 13

Urea agar

Question 14

Detection of Trichosporon spp

Answer 14

Urea agar

Question 15

Identification of yeasts by determining fermentation

Answer 15

Yeast fermentation broth

Question 16

Identification of yeasts by determining carbohydrate assimilation

Answer 16

Yeast nitrogen-base agar