Collection, Transport, and Culturing of Clinical Specimens Flashcards
most common specimens collected for fungal culture
Respiratory tract secretions
Respiratory Tract Secretions
● For optimal recovery of fungi, media without ___________ and a media containing ______________ should be used to prevent overgrowth of contaminants
● antifungal agent ______________ prevents overgrowth by rapidly growing molds and should be included in at least one of the culture media
● Specimens may be stored at ___________if processing is completed _____________; if processing is delayed, specimens should be _________________
Antibiotic
Antibacterial antibiotic
Cycloheximide
Room temp
Within 2 hours
be refrigerated at 4°C
Cerebrospinal Fluid (CSF)
● _____________ and the concentrated sediment used to inoculate the culture medium; cultures should be examined ___________
● _____________submitted for culture:
- centrifuged, ______________of the sediment should be placed on several areas on the agar surface
● CSF specimens should be processed ___________. If prompt processing is not possible, samples should be kept at ______________or placed in a ____________, CSF specimens should never be _______________
Centrifuged
Daily
<1ml spx
1 drop of aliquot
Promptly
Room temperature
30°C incubator
Refrigerated
Blood
● ___________provide an accurate method for determining the cause in many instances of disseminated fungal infections
Blood culture
Eye (Corneal Scrapings or Vitreous Humor)
● _______________: placed directly onto microscopic slides and inoculated onto noninhibitory media in either a ________________
● ________________: concentrated by centrifugation and the sediment should be used for smears and culture.
● Media containing ___________ should be avoided to prevent inhibition of potential isolates
Corneal scrapings
X- or C-shaped pattern.
Vitreous humor aspiration
cycloheximide
Hair, Skin, and Nail Scrapings
● Samples collected from lesions may be obtained by___________________________________;______________ are removed by plucking them with forceps.
● Only the __________________should be sampled, because the centers often contain nonviable organisms
● specimens should be placed in a _____________; they should not be refrigerated
● Cultures should be incubated for a minimum of ___________________ before being reported as ____________.
scraping the skin or nails with a scalpel blade or microscope slide
infected hairs
leading edge of skin lesions
Sterile container
21 days at 30°C
Negative
Vaginal
● should be transported to the laboratory within __________________ using ______________; swabs should be _____________ in sterile tubes
● Vaginal cultures should be screened for _______ and incubated at ___________?
within 24 hours of collection
culture transport swabs
Keep moist
Yeast
30°C for 7 days
Urine
● Urine samples collected for fungal culture should be processed ________________________; ________________ sample is unacceptable for culture
● All urine samples should be ______________ and the sediment cultured using a loop to provide adequate isolation of colonies.
● Because urine often is contaminated with __________________, media containing antibacterial agents must be used to ensure the recovery of fungi.
as soon after collection as possible;
24-hour urine
Centrifuged
Gram-negative bacteria
Urine
● If processing is completed ______________, samples may remain at ___________
● if processing is delayed, specimens should be _______________
● If a urine transport system is used, samples may be ______________________
Within 2 hours
Room temp
refrigerated at 4°C
stored at room temperature for up to 72 hours
Tissue, Bone Marrow, and Sterile Body Fluids
● All tissues should be processed ________________________; not grinding is critical
● Tissue pieces should be pressed into the appropriate culture media so that they are partially embedded, and the cultures should be _________________(___________ may be extended if clinical suspicion of a mycotic disease is high)
● ____________ may be collected in a ______________. The sample should be placed directly onto the surface of appropriate culture media and ___________________
before culturing by mincing
incubated at 30°C for 21 days
Incubation
Bone marrow
Heparinized syringe
30°C for 21 days
Tissue, Bone Marrow, and Sterile Body Fluids
● Sterile body fluids should be concentrated by _________ before culturing, and__________ specimen should be placed on the surface of appropriate culture media
● All specimens should be cultured ___________ to ensure the recovery of fungi.
Centrifugation
At least 1 ml
Asap
