Primary Cell Culture Of Outer Root Sheath Cells

Question 0

Mitomycin C

Answer 0

Antibiotic
Inhibits DNA synthesis by forming cross links between the complementary strands
Acts in late G1/early S
Way to make a feeder layer

Question 1

Feeder layer purpose

Answer 1

Layer of cells that the Po cells are seeded on top
Way to supplement media by metabolite leakage or secretion from the feeder cells
Condition the substate (feeder cells serve as a basal layer)

Question 2

Anlagen

Answer 2

Can last 2-6 years, 85% of follicles are in this stage

Question 3

Catagen

Answer 3

Lasts 1-2 weeks, 1% of follicles are in this stage

Apoptosis of the bottom 2/3

Question 4

Telogen

Answer 4

Lasts 5-6 weeks, 14% of follicles are in this stage

Resting phase, concentration dependent

Question 5

ORS

Answer 5

Undifferentiated keratinocytes

Question 6

Immersion media

Answer 6

Want a 8x concentration of Pen/Strep

Uses Hepes- allows yo visualize before going into actual media, pH independent (doesn’t rely on CO2 incubation)

Question 7

ORS culture medium

Answer 7

DMEM
Hams F12
FBS 
Pen/Strep 
Hydrocortisone 
Adenine 
Triiodothyronine 
Cholera toxin
Insulin
EGF

Question 8

Pen/Strep

Answer 8

Antibiotic against gram - and +

Question 9

Hydrocortisone

Answer 9

Hormone- physiological antagonist to insulin

Question 10

Adenine

Answer 10

Vitamin B4

Question 11

Triiodothyronine

Answer 11

Hormone- similar to the thyroid hormone thyroxine

Question 12

Cholera toxin

Answer 12

Suppresses the growth of fibroblasts

Question 13

Insulin

Answer 13

Hormone- regulates glucose uptake, utilization and storage

Question 14

EGF

Answer 14

Growth factor for growth, proliferation, and differentiation

Question 15

Teratoma

Answer 15

Tumor that contains more than 1 germ layer

Question 16

Advantages of using autologous ORS cells

Answer 16

Noninvasive isolation
Repeat sampling
Grow independent of donors age