HeLa Cells Exposed to Cisplatin and Mitomycin C

Question 1

E6

Answer 1

inactivates p53

Question 2

E7

Answer 2

inactivates pRB- prefers the active form of pRB

Question 3

E6 and E7 together effect

Answer 3

prevent apoptosis and promote cell cycle progression

Question 4

E6 and E7 together effect

Answer 4

prevent apoptosis and promote cell cycle progression

Question 5

Inactivation of p53 by E6

Answer 5

E6 and E6AP make a complex that acts as a ligase for p53

Question 6

Cisplatin

Answer 6

alkalating agent that prevents replication by forming cross links intrastandedly or interstrandedly of the DNA

Question 7

Mitomycin C

Answer 7

antibiotic
inhibits DNA synthesis by forming cross links between the complementary strands; therefore, the strands can’t separate during replication
acts in late G1/early S

Question 8

Stages of apoptosis

Answer 8

cell shrinkage –> nuclear condensation –> further condensation –> membrane blebbing, apoptotic bodies, and fragmentation of DNA

Question 9

EC50

Answer 9

the effective concentration of a drug that gives half-maximal response (death)