Cell Cycle and FLOW Analysis

Question 1

Digitonin

Answer 1

nonionic detergent that permeabilizes cellular and nuclear membranes

Question 2

RNase A

Answer 2

digest all RNA in cell, we want to only measure DNA

cleaves at the 3’ side of uracil or cytosine phosphate bonds

Question 3

Farnesyltransferase

Answer 3

recognizes the CAAX signal and add a 15 carbon isoprenoid group- which anchors EGFP to the inner face of the plasma membrane

Question 4

CAAX

Answer 4

cysteine-aliphatic aa (glycine, alanine, valine, leucine, isoleucine)-aliphatic aa-variable

Question 5

Look at cell cycle mixture

Answer 5

Digitonin, RNasa A, and PI