Basics of Cell Culture Flashcards
1
Q
Myoblast
A
muscle steam cells
2
Q
Laminar flow hoods
A
class II microbiological safety cabinets
3
Q
HEPA filters
A
High Efficiency Particulate Air
Removes 99.9% of particles 0.3 um or larger
Generally good for 10 years
The flow hoods have 2 HEPA filters
4
Q
Limitations of hoods
A
disrupt air flow by: coughing, quick movement, talking, heat sources, clutter
5
Q
CO2 water-jacketed incubators
A
keeps a constant temperature and avoids temperature fluctuations
6
Q
Types of chemical contamination
A
media sera water endotoxins storage vessels fluorescent lights incubators
7
Q
Types of biological contamination
A
bacteria, yeast, mold viruses protozoa invertebrates mycoplasma cross-contamination
