C2C12 Differentiation And ORS P1

Question 0

C2C12

Answer 0

Muscle myoblast
Need to be kept at <80% confluent (more than that differentiation starts)
Uses DMEM

Question 1

Cellular differentiation pathway

Answer 1

Undifferentiated stem cells –> precursor cell –> commitment –> differentiation –> terminal differentiation

Question 2

Commitment

Answer 2

Implies an irreversible progression that endows the cell the potential to express specific genes
The cells are able to proliferate indefinitely at this stage

Question 3

Differentiation

Answer 3

Expressing protein
The cell has progressed to a defined lineage
All genes necessary for that line are being expressed
The cell is at an irreversible stage

Question 4

Terminal differentiation

Answer 4

The cell no longer synthesizing DNA or dividing

It is considered a mature cell

Question 5

Myotonic regulatory factors

Answer 5

MyoD
Myf5
Myogenin
MRF4
All transcription factors

Question 6

MyoD

Answer 6

Master switch

Continually expressed throughout differentiation

Question 7

Myf5

Answer 7

Protein highly expressed in myoblasts

Question 8

Myogenin

Answer 8

Drive cell to differentiation state

Question 9

MRF4

Answer 9

Protein found in terminal muscle

Question 10

The myogenic pathway

Answer 10

Self regulating and cross talk

The positive loop amplifies the expression

Question 11

Tubulin

Answer 11

Housekeeping gene control because the levels stay constant