Phases of Cell Growth Flashcards
1
Q
Phases of growth
A
lag, logarithmic, stationary, decline
2
Q
Lag
A
cells are not dividing
low density
adaptation
3
Q
Logarithmic
A
active proliferation
cells are at their healthiest
exponential growth
90-100% in cell cycle
4
Q
Stationary
A
cell division and death are balanced cells are confluent cells are susceptible to injury <10% in cell cycle media turning orange (acidic) because lactic acid is produced
5
Q
Decline
A
due to cell death
cell death > cell division
media turning acidic
can’t save the cells, need to be thrown out
6
Q
Generation number
A
cells in culture will undergo sequential symmetric divisions such that the population number increases exponentially
n=3.32(logNh-logNi)
7
Q
Multiplication rate
A
number of generations that occur per unit time and is usually expressed as population doublings per 24 hours
r=3.32(logNh-logNi)/(t2-t1)
8
Q
Population doubling time
A
the time, expressed in hours, taken for cell number to double
PDT=1/r
