PRELIM: THE AMOEBAS

Question 1

INTESTINAL AMEBAE

Answer 1

Entamoeba histolytica

Question 2

COMMENSAL AMEBAE

Answer 2

Entamoeba harmanni
Entamoeba nana
Entamoeba butschlii
Entamoeba gingivalis
Entamoeba coli
Entamoeba polecki

Question 3

FREE-LIVING PATHOGENIC AMEBAE

Answer 3

Naegleria fowleri
Acanthamoeba species

Question 4

• pseudopod-forming non flagellated protozoan parasite
• MOST INVASIVE
   ONLY MEMBER to cause colitis and liver abscess
   Capable of ERYTHROPHAGOCYTOSIS

Answer 4

Entamoeba histolytica

Question 5

Shape: spherical
 Nucleus: 1-4
- PC: fine, uniform
- K: small, central – “bull’s eye” appearance
 Cytoplasm: with rod-shaped (or cigar-shaped) chromatoidal bars

Answer 5

Entamoeba histolytica CYST STAGE

Question 6

 Motility: progressive, unidirectional hyaline finger-like/blade pseudopod
 Nucleus: single, eccentric
- Peripheral chromatin (PC): fine uniform
- Karyosome (K): small, central
 Cytoplasm: fine, granular, ground-glass appearance, ingested RBCs

Answer 6

Entamoeba histolytica TROPHOZOITE STAGE

Question 7

Clinical Manifestation of Entamoeba histolytica

Answer 7

Amoebic Ulcer
Amoeboma
Amoebic Dysentery
Amoebic Liver Abscess

Question 8

Diagnostic test of Entamoeba histolytica

Answer 8

Microscopic detection (min. of 3 samples –different days)
Direct Fecal Smear
Formalin Ether Acetate Concentration Test (FECT)
Merthiolate Iodine Formalin Concentration Test (MIFCT)
Robinson’s and Inoki Stool Culture