practical: investigating enzymes Flashcards
what indicator would you use to test for the present of starch
iodine
what colour is positive test result for starch
blue/black
what colour is negative test result for starch
no colour change
what enzyme breaks down starch
amylase
if amylase were added to a starch solution what would you except to happen to the starch
it should be broken down
what piece of equipment would you ise to measure temperature
thermometer
what piece of equipment would you use to measure time
stopwatch
what piece of equipment would you use to keep the solutions at a certain temperature
water bath
around what temperature would you expect amylase to work best and why
37 degrees because its the temperature of the human body
aroun what ph does amylase work best
7
how frequently should you test your substance for the prescence of starch
30 seconds
at high temperatures why would you expect no starch to be broken down
the enzyme would have been denatured
at low and high phs why would you expect no starch to be broken down
the enzyme would have been denatured
why should you leave the solutions in the water for at least 5 minutes long
to allow the solutions time to get to the same temperature
why would you expect it to take longer for starch to appear at a low temperature
molecules have low kinetic energy and so fewer collisions between starch and amylase
