PRACTICAL Flashcards
Primary and secondary lymphoid structures
Primary
BM (HSC differentiate)
Thymus - upper chest behind breastbone. It’s the site of T cell maturation through selection and differentiation
Secondary
Lymph nodes - often located at at junctions of lymphatic vessels
Spleen - filters blood removing old or damaged RBC and is reservoir for lymphocytes and immune cells
Mucosa associated lymphoid tissue-MALT eg GI respiratory and urogenital. Helps protect these surfaces from pathogen and plays a role in mucosal IR
Do all lymph nodes have a blood supply
Yes-it’s the principle route of lymphocyte entry into lymph node
Enumeration of leukocytes in peripheral blood compared to spleen
Label this
Micro anatomy of lymph node 2L and changes w immunization
Outer cortex- contains b cells and GC
Paracortex- contains T cell Rich areas and HEV
Medulla- contains PC and macro
Changes-
GC in cortex expand and B cell proliferation increases
Paracortex expands- increased T cell activation and proliferation
Medullary- PC increase producing AG specific Ab
Micro anatomy of lymph node 2L and changes w immunization
Outer cortex- contains b cells and GC
Paracortex- contains T cell Rich areas and HEV
Medulla- contains PC and macro
Changes-
GC in cortex expand and B cell proliferation increases
Paracortex expands- increased T cell activation and proliferation
Medullary- PC increase producing AG specific Ab
Micro anatomy of spleen and changes in immunisation
White pulp- T cell rich periarteriolar lymphoid sheaths (PALS) and cont B follicles
Red pulp- cont blood filtering sinuses and macro
Marginal zone- between white pulp and red pulp cont specialised ,acro and lymphocytes
Changes -
expansion of. Follicles and GC in white pulp
Increase T cell act and prolif in. PALS
Expansion of marginal zone- inrease Ag trapping and processing
Increase production of PC in red pulp
MALT changes in response to immunisation
Label spleen
List organs and tissues which take up colloidal carbon after intravenous injection
List organs and tissues which take up colloidal carbon after intravenous injection
Underlying reason for carbon update
Conditions that affect size or function if secondary lymphoid organs
Do lymphoid tissue cont NK
Other leukocyte populations in lymphoid tissue
Two main areas in spleen and functions
Give 3 specific examples of tissue specific immune cells and their
Peripheral blood subsets- normal in adult
Romasnowsky staining protocol
Flow cytometry
= measurement of physical or chemical characteristics of biological particles whilst travelling through fluid stream and pass through interrogation point where they interact w laser light and emit a particular range of wavelengths to be collected in photomultipler tube. They are collected and amp, and digitalised. Can use Ab tagged with flourochromes.
Can identify DNA or membrane potential
Can recognise leukaemias, immunodeficiency’s, and viral infection due to changes in blood population.
Granularity vs size plot
Flow practice 2. What are you measuring
Read pink
Prac 2 answer Qs
Cheapest way to accurately determine number of cells in a sample
Calculation for haemocytometer
Prac 3 outline technique
Prac 3 outline technique
Elisa steps (Prac 3)
-binding of second Ab is proportional to what?
Prac 3
What protein property allows elisa plates to be Coated with Ag
Why is it important to block Elisa plate
What class of Ab does Elisa detect
Prac 3 answer questions
Prac 3 answer questions
Prac 4 design
How to score haemaggulation assay wells
What do inguinal, axillary and branchial lymph nodes respond to
Fill in
What does Elisa stand for and measure
What class of Ab does Elisa detect
Prac 3 - why produce curve
Prac 3 - why produce curve
Prac 3 answer
Prac 3
Describe ab neutralisation
Describe agglutination
Answer
Answer
Answer
Results of Elisa Prac 3
Agglutination diagram Prac 4
Name types of haem- agglutination assays
Ab for each blood group
Identify blood types
What’s viral haemagglutination inhibition test
Passive agglutination
Direct Coombs test
Indirect Coombs test
How do u score haemaggultination
Haemolysis assay
Quantative haemagglutination
Prac 4 premise
How to score haemagglutination
Prac 4
Answer 124
Row A= non immune rabbit, explain results
Give 4 examples of other cells in lymph nodes and 2 tissue specific immune cells
Give 4 examples of other cells in lymph nodes and 2 tissue specific immune cells
Romasnowsky stain
Romasnowsky stain
Hematoxylin and eosin stains
Basic idea of immunohistochemical staining
Identify leukocyte type from stains
What’s wrong w each patient
Staining blood smears vs tissue
What do hematoxylin and eosin stain
In peripheral blood ull find
Neutrophil smear
What do basophil and eosinophil smear look like
How to identify lymphocytes and monocytes on smear
Features of infectious disease on smear
