PQ, orlaith doc - final Flashcards
What is the optimal ewe ram ration at natural mating in natural cycle?
:50
- 1 ram per 50 ewes (50:1 ratio)
What is the concentration of the spermatozoa in case of laparoscopic AI?
20 million per uterine horn
- Intrauterine (Laparoscopic) 20 X 106 ( = 20 Million ) (into the lumen of the uterus)
What is the volume of the ram ejaculate?
1-2ml
Which component of the egg yolk protects the plasma membrane of the spermatozoa?
Lipoprotein
When does the cow ovulate?
10-12hrs after the end of oestrus
- Notes say 10-15 hours after the end of oestrus
Which insemination technique is not used in the pig?
Vaginal
- Cervical/ Uterus is used in pigs
What type of semen is used in the practice of AI in the pig?
Semen stored at 16-18°C
- Lower than < 10 degrees - death
The semen is collected in the pig with?
Gloved hand technique - every 5-7 days
How frequently can the semen be collected in the pig
Max twice per week (every 5 -7 days)
Concentration of spermatozoa in the insemination dose of a pig?
3-5 billion
(says 3 billion of spermatozoa in insemination dose in notes)
Volume of insemination dose used in a pig?
50-100ml
(70 ml is the most used)
Ejaculation can be induced with ______________? Indicate the correct answer.
Ketamine??
Don’t know the options and what animal but
For Boar it can be
Manual induction:
Firm pressure on the glans penis –> Stimulation of sensory-R on the surface –> Ejaculation
And for Stallion it’s
Pharma induction:
Xylazine slow iv (0.66 mg/kg) with or without prior administration of imipramine iv. (2.2 mg/kg)
Imipramine per os (2.2 mg/kg), followed 2 hours later by xylazine slow iv. injection (0.2 – 0.3 mg/kg)
What is the concentration of the spermatozoa in the AI dose used in the horse?
500 million?
Routine work: 200-500 X 10^6 total sperm (initially from chilled, extended semen 1000- 2000 X 10^6 in 1050 mL, nowadays min.100 X 10^6 motile)
What is the correct time of the AI in the horse?
Close to the end of oestrus (behavioural oestrus should be present)
- 20 mins after collection if it’s fresh semen
What is the AI dose used in the horse?
10-50ml
Freezing container: straw (0.25, 37oC, 30 sec vs. 0.5ml, 2.5-4-5ml straw, 50oC, 45 sec
Collected semen must be diluted to 25 – 50 X10^6/ml before motility evaluation
Where should the semen be deposited in the mare?
Body of the uterus
Where does the dog ejaculate the the semen?
Vagina
Ru; Bull, ram and buck –> Into the deepest point of the vagina
Stallion and Boar –> Into body of the uterus
What is the acceptable motility result in the dog?
> 85% (75-90%)
How can the semen be collected from the stallion?
Artificial Vagina (AV)
Which properties of the AV are important at the semen collection in the stallion?
Temperature and pressure
AI of the mare should be done….?
Every other day or daily until the end of oestrus
Does the stallion ejaculate in different fractions?
Yes
Dog, Boar and Stallion
Which technique is not used for semen collection from the stallion?
Electroejaculation (its used mainly in sheep but rare in other small Ru)
- Used in Stallions: either AV pr Pharma induced ejaculation
How many fractions does the pig ejaculate?
3 fractions (sperm released in 2nd fraction )
Optimum time of AI in the pig?
In the middle of the 50-60h oestrus period
- AI should be done in the middle of the oestrous period (ranges from 12-96 hours)
What influences the changes in vaginal epithelium during the heat?
Oestrogen
Anterior vaginal epithelial cells proliferate and secrete mucus – Copious clear, A spike in Estrodiol includes the presence of a clear vaginal discharge.
Mark the incorrect statement regarding clinical and biochemical alterations in pregnant bitch
Constantly decreasing energy requirements
Clinical and biochem alterations in pregnant bitch:
- Increased plasma volume sufficient to cause a normo- chromic, normo-cytic anemia
- The high Ht - begin decreasing between day 25-30, reaching nadir near or below 40% at term, and anaemia is not resolved until 1–2 months postpartum
- Hypercholesterolemia and hyperproteinemia
- Increased insulin resistance, normal feature of the pregnancy, causes –> progesterone
o (DM may develop in diestrus in non-pregnant bitches) - Mild leukocytosis ( increased white blood cell count) without a left shift - no immature neutrophils
o Increased fibrinogen and c-reactive protein, other alterations in inflammatory and coagulation factors
What is not a sign for impending parturition in the bitch? Mark the incorrect statement
Sudden increase in body temperature by 1-2°C
Impending parturition signs in bitch:
- Behaviour: restlessness, nest building
- Vuvla become swollen and edematous
- Vaginal discharge
- Relaxation of abdominal and pelvic muscles
- Swollen mammary gland, milk secretion
- 18 – 30 hours before parturition, the plasma P4 concentration declines below 2 ng/ml
- 10 – 14 hours after this hormone change, the rectal temperature of the bitch falls below 37.2 *C (approx. 24 hours before whelping)
What is your suspected diagnosis in case of mucoid vaginal discharge in a pregnant dog
Sign of impending parturition
How long is the dog’s pregnancy?
62-63 days - Around closer to 65 days
What is the meaning of preovulatory luteinization?
The plasma progesterone (p4) level starts to increase before the ovulation
Mark the incorrect statement. Which factor does NOT predispose to Primary Uterine Inertia?
High humidity
Predisposition to Primary Uterine Inertia:
§ Breed predisposition (border terrier, Aberdeen terrier)
§ Overstretching of the uterus containing a large litter
§ Inadequate uterine stimulation in one or two pup litters
§ Systemic disease such as hypocalcemia, hypoglycemia, ketosis or
infection
§ Inadequate nutrition
§ Obesity
§ Stress
§ Uterine torsion
Which method can we NOT use for artificial insemination if we use frozen semen?
Intravaginal
What progesterone level is expected at day 0 of oestrus cycle (LH peak) in the dog?
Around 2 ng/ml at LH
Then rises to 6 ng/ml at ovulation
When do you NOT necessarily have to think about dystocia?
The bitch has been panting, trembling, restless for 6 hours but nothing else
Which statement is FALSE concerning the hormonal background of the luteal phase in bitches?
Low LH levels can cause abortion
- causes infertility?
How does the plasma progesterone change after ovulation?
Increases
Rapidly rising peripheral P4 levels that reach their highest levels (30-35 ng/ml (although sometimes displaying values of 80-90 ng/ml or hig her)) usually within 15-30 days after ovulation.
Which factor does NOT influence the success of AI?
The weather on the day
Mark the incorrect statement regarding clinical pseudopregnancy
The use of dopamine antagonist as primary medication is recommended
- Dopamine AGONISTS are recommended
See here:
Prolactin Inhibitors - dopamin agonists - Bromocriptine, administered orally at 0 .01 to 0 .1 mg/kg/day individed doses, should be given until lactation ceases
–> Common side effects- vomiting, anorexia, depression, and behavioural changes - Cabergoline, administered at a dose of 5 μg/kg once daily for 5 to 10 days
–> better-tolerated ergoline compound Side effects were considered mild and included lethargy, inappetence, and vomiting - Progestin administration is contraindicated!!! OE/OHE recommended in any pseudopregnant dog
Which statement is correct? Clinical signs of proestrus in dogs are induced by..
Increase of Estrogen
Clinical signs:
- Appearance of bloody discharge
- E2 peaks, and then drops going into oestrus
Which is a misjudgement of foetal heart rate?
100-150 BPM means moderate foetal distress
- More than 220 beats per minute
- 180 220 bpm = moderate foetal distress
- Less than 180 = severe foetal distress
What can be the consequence of spaying a dog in diestrus?
overt pseudopregnancy - “Physiologically, all non-pregnant bitches in diestrus are pseudopregnant”
Reasoning:
There is a mid-late luteal phase increase in PRL-dependent stimulation of mammary tissues
- Some non-pregnant bitches are more sensitive than others to these normal endocrine events, resulting in the exhibition of obvious pseudopregnancy
- Mean PRL is higher and mean P4 lower in effected cycles
- Ovariectomy (during luteal phase) , PGF induced luteolysis or antiprogestin administration can each increase PRL
Mark the incorrect statement. What happens by the end of canine pregnancy?
The relaxin concentration is high
Relaxin becomes detectable around day 20-25 of pregancy, peak at 2-4 weeks before birth and stays elevated until term
When does the bovine embryo begin to hatch?
Day 9 to 11 - hatching from the zone pellucida in the fetal development of bovine
What does capacitation mean?
The spermatozoa acquire the hyperactive motility
What does acrosome reaction mean?
Fusion of outer acrosomal membrane with plasma membrane of spermatozoa
What is the result of acrosome reaction?
Formation of zona pellucida block
What does polyspermiosis mean?
More than one spermatozoa penetrating the oocyte
What is the location of the fusion proteins on the spermatozoa?
The equatorial segment which can be found on the head of spermatozoa
What is the task of the fusion proteins?
To secure the physical connection between the head of the spermatozoa and the plasma membrane of the oocyte –> responsible for formation and preparation between head of spermatozoa and oolemma of the oocyte