Potassium

Question 1

Principle for potassium

Answer 1

Pyruvate kinase is activated by K+ ions in the sample and subsequently catalizes the dephosphorylation of phosphoenol- pyruvate to pyruvate. In a second step pyruvate is transformed to Lactate under consumption of a NADH analogue. The rate of the signal decrease measured at 340 nm is proportional to the amount of potassium in the sample.

Question 2

R1 for potassium

Answer 2

Buffer
NADH analogon
Phosphoenolpyruvate ADP
Lactate dehydrogenase

Question 3

R2 for potassium

Answer 3

Buffer ph7.0
Pyruvate kinase

Question 4

Limit of detection

Answer 4

0.4 mmol/L

Question 5

On board stability

Answer 5

4 weeks

Question 6

Calibration stability

Answer 6

7 days

Question 7

Reference range for potassium in plasma

Answer 7

3.6 – 4.8 mmol/L

Question 8

Reference range for potassium in serum

Answer 8

3.5 – 5.1 mmol/L

Question 9

Wavelength for potassium

Answer 9

340

Question 10

Limit of detectiom for potassium

Answer 10

0.4 mmol/L

Question 11

Measuring range for potassium

Answer 11

2-8 mmol/L

Question 12

Potassium conversion factor

Answer 12

3.91