Chloride

Question 1

Method for chloride

Answer 1

Photometric test using ferric (III) perchlorate

Question 2

Principle for chloride

Answer 2

Chloride forms with ferric ions a yellow colored complex whose absorption is measured at 340 nm. A discoloring agent in reagent 2 displaces Chloride out of the complex, thereby discoloring the solution. The difference in absorbance between the colored and discolored state of the solution is proportional to the concentration of chloride in the sample.

Question 3

Reagents for chloride

Answer 3

R1: Methanesulfonic acid Ferric (III) perchlorate
R2: Inorganic salt

Question 4

Specimen for chloride

Answer 4

Serum or plasma (lithium heparin)
Separate from cellular contents immediately after blood collection.

Question 5

Wavelength for chloride

Answer 5

340/660 nm (bichromatic)

Question 6

Conversion factor for chloride

Answer 6

3.545

Question 7

Reference range for chloride

Answer 7

95-105 mmol/L