Magnesium Flashcards
Reagents for magnesium
Ethanolamine pH 11.0 750 mmol/L
GEDTA (Glycoletherdiamine-tetraacetic acid) 60 umol/L
Xylidyl blue 110 umol/L
Method for magnesium
Photometric test using xylidyl blue
Specimen for magnesium
serum, plasma, cerebrospinal fluid or urine
Wavelength for magnesium
520 nm, Hg 546 nm, 500 - 550 nm (Increase of absorbance)
628 nm, Hg 623 nm, 570 - 650 nm (Decrease of absorbance)
Measurement magnesium
Against reagent blank
Conversion factor for magnesium
0.4114
Refence range for magnesium
Women 1.9-2.5 mg/dL (0.77-1.03 mmol/L)
Men 1.8-2.6 mg/dL ( 0.73-1.06 mmol/L)
Reference range in magnesium for urine and csf
Urine: 73-122 mg/24 hr (3-5 mmol/24hr)
CSF: 2.1-3.3 mg/dL (0.85-1.35 mmol/L)
Principle of magnesium
Magnesium ions form a purple colored complex with xylidyl blue in alkaline solution. In presence of GEDTA, which complexes calcium ions, the reaction is specific. The intensity of the purple color is proportional to the magnesium concentration.
Decreased magnesium concentrations
Neuromascular irritability (tremor, seizures)
Cardiac symtonps ( tachycardia, arrythmia)
Decreased magnesium concentrations are often related to
Decreased calcium and potassium levels taking into hypomagnesemia may be primary cause of hypocalcemis
Elevated magnesium values
Dehydration
Renal disorder
After intake of excessive amount od antacids
Stored at
2-8 degree celcius
Inferences
Hemoglobin
Hormones associated with Magnesium
Parathyroid Hormones
Aldosterone and Thyroxine