Polymorphisms (Exam 1)

Question 1

{ }% of DNA is similar amongst individuals, but the extra { }% makes the difference.

Answer 1

1) 99.9
2) 0.1

Question 2

What polymorphism is DNA sequencing used for?

Answer 2

Single nucleotide polymorphism (SNP)

Question 3

What polymorphism is PCR used for?

Answer 3

Short tandem repeat (STR)

Question 4

What polymorphism is Restriction Fragment Length Polymorphism (RFLP) used for?

Answer 4

Variable number of tandem repeats

Question 5

Which polymorphism is a sequence polymorphism?

Answer 5

SNP

Question 6

Which polymorphisms are length polymorphisms?

Answer 6

STRs & VNTRs

Question 7

Approximately 3 million of this polymorphism have been identified in the human genome (1 per kb) and is considered the one specific base difference between 2 individuals.

Answer 7

What are SNPs?

Question 8

What is the range number of bases per repeat for STRs?

Answer 8

2-9

Question 9

What is the range number of repeats in a locus for STRs?

Answer 9

5-20, or 10-30 depending on who you ask

Question 10

What is the range number of bases per repeat for VNTRs?

Answer 10

10-100 or even more

Question 11

What is the range number of repeats in a locus for VNTRs?

Answer 11

50-100 or even more

Question 12

What is the difference in the quality of DNA used for PCR and VNTR?

Answer 12

PCR - DNA can be degraded and poopy
VNTR - DNA needs to be good quality

Question 13

What is the difference in the price for PCR and VNTR equipment?

Answer 13

PCR - very expensive
VNTR - ~$1.50 per test (very cheap)