DNA Detection Systems (Exam 3)

Question 1

This detection system is associated with the silver staining of gels.

Answer 1

What is acrylamide?

Question 2

This detection system is associated with flat bed scanners (HINT: Vibrator).

Answer 2

What is Hitachi FM Bio?

Question 3

This detection system is associated with laser-scanned fragments that come out of the gel.

Answer 3

What is ABI 373/377 sequencers

Question 4

This detection system is associated with capillary electrophoresis.

Answer 4

What is ABI 310, 3100, 3130

Question 5

How many buffer vials are in the ABI 310?

Answer 5

2

Question 6

How many samples are loaded into the ABI 310?

Answer 6

Variable

Question 7

How many coupled-charge device (CCD) cameras are in the ABI 310?

Answer 7

1

Question 8

What is the diameter of an ABI 310 capillary?

Answer 8

50 microns

Question 9

How long is the ABI 310 capillary?

Answer 9

47 cm

Question 10

What substance is injected into the ABI 310 capillary before adding the sample?

Answer 10

Performance-optimized polymer, 4 (POP-4)

Question 11

In the 310 capillary electrophoresis system, during electrophoresis, the fluorescent PCR fragments are excited by an… (HINT: 3 words).

Answer 11

Argon ion laser

Question 12

What are the 8 sample data analysis parameters (HINT: Regina Sucked Thad’s Cock Back [on] Monday [and] Kissed [his] Nuts)?

Answer 12

1) Range of analysis 0-3200/6000 scans
2) Size standards 75 to 450bp
3) Threshold 100 rfu cut off
4) Colors ROYGB
5) Baseline
6) Matrix file
7) Kit name
8) Number of colors

Question 13

In an electropherogram, the big dye blob in the beginning of the raw data is due to…

Answer 13

Unused fluorescent primers