Multiplex PCR (Exam 2 & 3) Flashcards
What are 5 ways to prevent DNA contamination?
1) Gloves
2) Designated pipette tips
3) Laminar flow hood
4) 10% bleach solution
5) UV light
The major benefit of multiplex PCR is that it saves { } and { }.
1) Time
2) Samples
Why does non-human DNA not interfere with PCR?
Primer specificity
What is the difference between single-plex and multiplex PCR in terms of MARKERS and TARGET SEQUENCES?
Single-plex PCR has one pair of markers amplifying a target while multiplex has multiple markers target multiple sequences
What is the purpose of a reagent blank in DNA extraction and PCR?
To ensure that reagents aren’t contaminated with DNA
What does one large peak on an electropherogram mean? Two smaller peaks?
1 peak: homozygous
2 peaks: heterozygous
What is the purpose of a negative control (HINT: remember that reagents have no DNA)?
To show if reagent has been contaminated with DNA.
What is the purpose of a positive control?
To test integrity of DNA sample against a known DNA profile
How many capillaries and electrodes are in the ABI 310 Genetic Analyzer?
1 Capillary, 2 electrodes
What are 2 advantages of the ABI 310 Genetic Analyzer?
1) Automated gel pouring
2) Automated sample injection
This man is responsible for validating STR research.
Who is Bruce Budowle?
Bruce Budowle is the executive director of this institute.
What is the Institute of Investigative Genetics?
