DNA Extraction (Exam 1) Flashcards
1
Q
The amount of DNA extracted from a cell depends on { }.
A
The number of nucleated cells present
2
Q
Organic extraction uses { } solvents to separate DNA.
A
Organic
3
Q
What is the difference between straight and differential extraction?
A
Straight extraction lyses everything (blood, tissues, hair, etc.), but differential extraction separates male and female cells
4
Q
What is the purpose of dithiothreitol (DTT)?
A
Breaks sulfide bonds of sperm’s acrosome