Commercial Kits Used for Human Identification (Exam 2 & 3)

Question 1

For a 15 marker autosomal STR analysis, and one amelogenin marker, how many alleles are amplified?

Answer 1

32

Question 2

In the Identifiler STR analysis kit that amplifies 15 STR markers and 1 amelogenin marker, how many primers are included? Break this number down.

Answer 2

-32 primers

-2 primers for each STR marker (2 * 15)

-2 primers for the amelogenin (2 * 1), which covers X and Y chromosomes

Question 3

The Applied Biosystems Identifiler STR and Promega Powerplex Fusion 6C kits are { }-color detection systems.

Answer 3

6

Question 4

The Promega Powerplex 35GY 8C system is a { }-color detection system.

Answer 4

8

Question 5

The Identifiler Human ID kit contains this many STR markers and this many amelogenin markers.

Answer 5

-15 STR

-1 Amelogenin

Question 6

The term “plex” is associated with this company.

Answer 6

What is Promega?

Question 7

The term “filer” is associated with this company.

Answer 7

What is Applied Biosystems?

Question 8

What are the 3 types of STR repeats (HINT: think sentences)?

Answer 8

Simple, compound, and complex

Question 9

Name this type of STR repeat: (AATG)5-11

Answer 9

Simple

Question 10

Name this type of STR repeat: TCTA (TCTG)3-4 (TCTA)n

Answer 10

Compound

Question 11

Name this type of STR repeat: (TCTA)4-6 (TCTG)5-6 (TCTA)3 TA(TCTA)3 TCA(TCTA)2 TCCATA(TCTA)8-16 TC

Answer 11

Complex