plant responses Flashcards

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1
Q

What are the differences and
similarities between chemical
coordination in plants and
humans?

A
• The key limitations on plants is 
that they are not mobile, and they 
don’t have a rapidly responding 
nervous system 
• Most plant responses are slower 
than animal responses, but they 
still respond as a result of complex 
chemical interactions 
• Plants have evolved a system of 
hormones
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2
Q

List some plant hormones

A
  • Auxins
  • Gibberellin
  • Ethene
  • ABA (abscisic acid)
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3
Q

What are the roles of auxins?

A
Control cell elongation 
• Prevent lead fall (abscission)
• Maintain apical dominance 
• Involved in tropisms 
• Stimulate the release of ethene 
• Involved in fruit ripening
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4
Q

What are the roles of

gibberellin?

A
Causes stem elongation
• Triggers the mobilisation of food 
stores in a seed at germination 
• Stimulates pollen tube growth in 
fertilisation
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5
Q

What are the roles of ethene?

A

• Causes fruit ripening
• Promotes abscission in deciduous
trees

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6
Q

What are the roles of ABA

(abscisic acid)?

A
• Maintains dormancy of seeds and 
buds 
• Stimulates cold protective 
responses e.g. antifreeze 
production 
• Stimulates stomatal closing
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7
Q

Why are scientists still unsure
about the details of many plant
responses?

A
• Plant hormones work at very low 
concentrations, so isolating them 
and measuring changes in 
concentrations is not easy 
• The multiple interactions between 
the different chemical control 
systems also make it difficult for 
researchers to isolate the role of a 
single chemical in a specific 
response
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8
Q

What must happen for a plant

to start growing?

A

The seed must germinate

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9
Q

Describe the process of seed

germination

A
1. When the seed absorbs water, 
the embryo is activated and 
begins to produce gibberellins
2. The gibberellins stimulate the 
production of enzymes that 
break down the food stores 
found in the seed
3. The embryo plant uses these 
food stores to product ATP for 
building materials so it can grow 
and break out through the seed 
coat 
4. Evidence suggests that 
gibberellins switch on genes 
which code for amylases and 
proteases (digestive enzymes 
required for germination)
5. Evidence also suggests that 
ABA acts as an antagonist to 
gibberellins, and that it is the 
relative levels of both hormones 
which determine when a seed 
will germinate
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10
Q

Where is the food store found

in seed?

A
  • Cotyledons in dicot seeds

* Endosperm in monocot seeds

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11
Q

Give experimental evidence
that supports the role of
gibberellins in seed
germination

A
• Mutant varieties of seeds have 
been bred without the gene that 
enables them to make gibberellins, 
and these seeds don’t germinate. 
If gibberellins are applied 
externally, then they germinate 
normally 
• If gibberellin biosynthesis 
inhibitors are applied to the seed, 
they don’t germinate as they can’t 
make gibberellins
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12
Q

Describe the auxins that play a
role in the growth of a plant
shoot

A
• e.g. indoleacetic acid (IAA)
• Growth stimulants produced in 
plants 
• Small quantities can have powerful 
effects 
• Made in cells at the tips of roots 
and shoots, and in the meristems
• Can move down the stem and up 
the root both in the transport 
tissue, and from cell to cell
• Effect of the auxin depends on its 
concentration and interaction with 
other hormones
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13
Q

What are the effects of auxin

on plant growth?

A
• They stimulate the growth of the 
main, apical shoot 
• They affect the plasticity of the cell 
wall - the presence of auxins 
means the cell wall stretches more 
easily 
• Auxin molecules bind to specific 
receptor sites in the plant cell 
membrane, causing the pH to fall 
to 5 
• Optimum pH needed for enzymes 
to keep the walls very flexible and 
plastic 
• As cells mature, auxin is destroyed 
and the pH rises so the enzymes 
maintaining plasticity become 
inactive 
• As a result, the wall becomes rigid 
and fixed in shape and size, and 
cells can no longer expand and 
grow
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14
Q

What is the effect of high

concentrations of auxins?

A
• Suppresses the growth of lateral 
shoots, resulting in apical 
dominance 
• Growth in the main shoot is 
stimulated by auxin produced at 
the tip, so it grows quickly 
• The lateral shoots are inhibited by 
the hormone that moves back 
down the stem, so they don’t grow 
very wells 
• Further down the stem, the auxin 
concentration is lower, and so the 
lateral shoots grow more strongly
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15
Q

Give some experimental
evidence for the role of auxins
in apical dominance

A
• If the apical shoot is removed, the 
auxin-producing cells are removed 
so there is no auxin 
• The lateral shoots grow faster 
• If auxin is applied artificially, apical 
dominance is reasserted, and 
lateral shoot growth is suppressed
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16
Q

What is the effect of low

concentrations of auxins?

A
• Promote root growth 
• Up to a given concentration, the 
more auxin that reaches the root, 
the more they grow 
• Auxin is produced by the roots tips 
and also reaches the roots in low 
concentrations from the growing 
shoots
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17
Q

Give some experimental
evidence for the role of auxins
in root growth

A
If the apical shoot is removed, the 
amount of auxin reaching the roots 
is greatly reduced, and root growth 
slows and stops 
• Replacing the auxin artificially at 
the cut apical shoots restores the 
growth of the roots 
• High auxin concentrations inhibit 
root growth
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18
Q

Effect of different
concentrations auxin on
different tissues

A
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19
Q

What role do gibberellins play

in plant growth?

A
They are important in the elongation 
of plant stems during growth 
• They affect the length of the 
internodes - the regions between 
the leaves on a stem
• Plants that have short stems 
produce few or no gibberellins 
• This reduces waste, and also 
makes the plants less vulnerable 
to damage by weather and 
harvesting
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20
Q

What is synergism?

A

When different hormones work
together, complementing each other
and giving a greater response than
they would on their own

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21
Q

What is antagonism?

A
When different hormones have 
opposite effects, e.g. one promoting 
growth and one inhibiting it, the 
balance between them will 
determine the response of the plant
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22
Q

Give examples of abiotic

stresses faced by plants

A
  • Changes in day length
  • Cold and heat
  • Lack of water
  • Excess water
  • High winds
  • Changes in salinity
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23
Q

Why do deciduous plants lose

their leaves?

A
• In temperate climates a point 
comes when the amount of 
glucose required for respiration to 
maintain the leaves, and to 
produce chemicals from 
chlorophyll that might protect 
them against freezing, is greater 
than the amount of glucose 
produced by photosynthesis 
• Also, a tree with leaves is more 
likely be blown over by winter 
winds
24
Q

Describe daylength sensitivity

in plants?

A
Photoperiodism - plants are 
sensitive to a lack of light in their 
environment 
• Many different plant responses are 
affected by the photoperiod, 
including the breaking of the 
dormancy of the leaf buds so they 
open up, the timing of flowering in 
a plant, and when tubers are 
formed in preparation for 
overwintering 
• Sensitivity of plants to day length 
(or dark length) results from a lightsensitive pigment called 
phytochrome
• Phytochrome exists in two forms 
(Pr and Pfr) - each absorbs a 
different type of light and the ratio 
changes depending on the levels 
of light
25
Q

What causes abscission/leaf

fall in plants?

A
• The falling light levels result in 
falling concentrations of auxin 
• Leaves respond to falling auxin 
concentration by producing the 
gaseous hormone ethene
• Ethene initiates gene switching in 
the cells in the abscission zone, 
resulting in the production of new 
enzymes 
• These enzymes digest and 
weaken the cell walls in the outer 
layer of the abscission zone, 
known as the separation layer
• The vascular bundles which carry 
materials into and out of the leaf 
are sealed off
• Fatty material is deposited in the 
cells on the stem side of the 
separation layer, forming a 
protective scar when the leaf falls, 
to prevent the entry of pathogens 
• Cells deep in the separation zone 
respond to hormonal cues by 
retaining water and swelling, 
putting more strain on the already 
weakened outer layer 
• Abiotic factors e.g. low 
temperatures and strong winds 
finish the process
• The leaf separates from the plant 
• A neat, waterproof scar is left 
behind
26
Q

Why do plants try to prevent

freezing?

A

If cells freeze, their membranes are

disrupted and they will dies

27
Q

How do plants prevent

freezing?

A
• The cytoplasm of the plant cells 
and the sap in the vacuoles 
contain solutes which lower the 
freezing point 
• Some plants produce sugars, 
polysaccharides, amino acids and 
proteins which act as antifreeze to 
stop the cytoplasm from freezing, 
or protect the cell from damage 
even if they do freeze
28
Q

How are the stomata

controlled?

A

• The hormone ABA
• Leaf and root cells release ABA
under abiotic stress, causing
stomatal closure

29
Q

What happens when the plant

is under abiotic stress?

A
• Plant roots produce ABA which is 
transported to the leaves 
• At the leaves it binds to receptors 
on the plasma membrane of the 
stomatal guard cells 
• ABA activates changes in the ionic 
concentration of the guard cells, 
reducing the water potential and 
turgor of the cells
• Because of reduced turgor, the 
guard cells close the stomata, and 
water loss by transpiration is 
reduced
30
Q

What is herbivory?

A

The process by which herbivores eat

plants

31
Q

What physical defences do

plants have to herbivory?

A

horns, barbs, spikes, spiny leaves,
fibrous and inedible tissue, hairy
leaves and stings

32
Q

What are the chemical
defences plants have to
herbivory?

A
  • Tannins
  • Alkaloids
  • Terpenoids
33
Q

What are tannins?

A
• Part of a group of compounds 
called phenols 
• Can make up 50% of the weight of 
the leaves 
• Very bitter taste which puts 
animals off eating the leaves 
• Toxic to insects - bind to the 
digestive enzymes in their saliva 
and inactivate them
34
Q

What are alkaloids?

A
Large group of very bitter tasting 
nitrogenous compounds 
• Act as drugs, affecting the 
metabolism of animals that take 
them in and sometimes poisoning 
them 
• e.g. caffeine, nicotine, morphine, 
and cocaine 
• Caffeine is toxic to fungi and 
insects, and it prevents the 
germination of other seeds in the 
soil, so no competition from plant 
rivals 
• Nicotine is produced in the roots 
of tobacco plants, and transported 
to the vacuoles of the leaves, 
where it is stored. It is released 
when the leaf is eaten
35
Q

What are terpenoids?

A
Large group compounds which 
often form essential oils, but also 
act as toxins to insects and fungi 
• Pyrethrin, produced by 
chrysanthemums, acts as an 
insect neurotoxin 
• Citronella, produced by lemon 
grass, repels insects
36
Q

What are pheromones?

A
Chemicals made by an organism 
that affect the social behaviour of 
other members of the same species
• Plants don’t behave socially, so 
don’t rely a lot on pheromones
37
Q

Give examples of where plants
use pheromones to defend
themselves

A
If a maple tree is attacked by 
insects, it releases a pheromone 
which is absorbed by leaves on 
other branches 
• these leaves them make chemicals 
e.g. callose to protect themselves if 
they are attacked
38
Q

What are volatile organic

compounds (VOCs)?

A
Chemicals produced by plants 
which act like pheromones between 
themselves and other organisms, 
particularly insects. Some VOCs 
may act as a ‘pheromone’ so that 
neighbouring plants begin to 
produce the VOC before being 
attacked 
• Usually only made the the plant 
detects attack by an insect pest 
through chemicals in the saliva of 
the insects 
• This may elicit gene switching
39
Q

Give examples of plants

producing VOCs

A
• When apple trees are attacked by 
spider mites, they produce VOCs 
which attract predatory mites that 
come and destroy the apple tree 
pests 
• Some types of wheat seedling 
produce VOCs when they have 
been attacked by aphids, and 
these repel other aphids from the 
plant
40
Q

Describe plants that fold in

response to touch

A
Mimosa pudica 
• If the leaves are touched, they fold 
down and collapse 
• This frightens off larger herbivores, 
and dislodges small insects which 
have landed on the leaves 
• The leaf falls in a few seconds, 
and recovers over 10-12 minutes 
as a result of potassium ion 
movement into specific cells, 
followed by osmotic water 
movement
41
Q

What are tropisms?

A
Plant growth responses to stimuli 
from one direction .
• Phototropism - the growth of 
plants in response to light which 
comes from one direction only 
• Geotropism - the response to 
gravity 
• Chemotropism - the response to 
chemicals 
• Thigmotropism - the response to 
touch
42
Q

How are tropisms a response

to environmental cues?

A
• Once a seed begins to germinate 
in the soil, the shoot must grow up 
towards the light sources for 
photosynthesis, and the roots 
must grow downwards into the soil 
which will provide support, 
minerals and water
43
Q

Why does most research on
tropisms use germinating
seeds and young seedlings?

A
• They are easy to work with and 
manipulate 
• They are growing and responding 
rapidly, so any changes show up 
quickly 
• Changes tend to affect the whole 
organism rather than a small part, 
making any tropisms easier to 
observe and measure
44
Q

Why are seedlings of
monocotyledonous plants
usually used?

A
• e.g. cereals like oats and wheat 
• The shoot that emerges is a single 
spike with no apparent leaves 
known as coleoptile 
• Easier to manipulate and observe 
than a dicotyledonous shoot 
• Coleoptiles are relatively simple 
plant systems, so its important to 
remember that the control of the 
responses to light in an intact adult 
plant may be more complex
45
Q

What are phototropisms

caused by?

A
The movement of auxins across the 
shoot or root if it is exposed to light 
that is stronger on one side than the 
other 
• The shoots of plants grow towards 
the light, and the roots (if exposed) 
grow away 
• Shoots are positively phototropic 
• Roots are negatively phototropic
46
Q

What is the survival value of

phototropism?

A
• Helps ensure that the shoots 
receive as much all-round light as 
possible, allowing the maximum 
amount of photosynthesis to take 
place
• If the roots emerge from the soil 
(e.g. after particularly heavy rain) 
they will rapidly turn back into the 
soil
47
Q
What is the effect of the 
following? 
1. Removing shoot tip
2. Lightproof cover placed over 
intact tip of shoot 
3. Thin impermeable barrier of 
mica 
4. Mica inserted on shaded side
5. Tip removed, gelatine block 
inserted and tip replaced
A
1. No response: The tip must either 
detect the stimulus or produce 
the messenger (or both) as its 
removal prevents any response 
2. No response: The light stimulus 
must be detected by the tip
3. Bends towards light: Mica on the 
illuminated side of the shoot 
allows the hormone to pass only 
down the shaded side where it 
increases growth and causes 
bending 
4. No response: Movement of 
chemical down shaded side is 
prevented by mica 
5. Bends towards the light: As 
gelatine allows chemicals to 
pass through it, but not electrical 
messages, the bending which 
occurs must be due to a 
chemical passing from the tip
48
Q

What is the effect of unilateral

light?

A
Where plants are partially shaded 
the shoots grow towards the light 
and then grow on straight towards it
1. The side of a shoot exposed to 
light contains less auxin than the 
side which is not illuminated 
2. The light causes auxin to move 
laterally across the shoot, so 
there is a greater concentration 
on the un-illuminated side 
3. The stimulates cell elongation 
and growth on the dark side, 
resulting in observed growth 
towards the light 
4. Once the shoot is growing 
directly towards the light, the 
unilateral stimulus is removed 
5. The transport of auxin stops, and 
the shoot then grows straight 
towards the light
49
Q

Why do plants grow more
rapidly in the dark than when
they are illuminated?

A
If a plants in the dark, the aim is to 
grow upwards rapids to reach the 
light to be able to photosynthesise
• The seedlings that break through 
the soil first won’t have to 
compete with other seedlings for 
light 
• Evidence suggests that gibberellin 
are responsible for the extreme 
elongation of the internodes when 
a plant is grown in the dark 
• Levels of gibberellin fall when the 
stem is exposed to light
50
Q

Why is the slowing of upwards
growth when exposed to light
valuable?

A

• Resources can be used for
synthesising leaves, strengthening
stems, and overall growth

51
Q

Why is geotropism important?

A
• In normal conditions, plants 
always receive a unilateral 
gravitational stimulus - gravity 
always acts downwards 
• Shoots are negatively geotropic 
(grow away from gravitational 
pulls)
• Roots are positively geotropic 
(grow towards gravitational pull)
• Geotropism is also known as 
gravitropism
52
Q

List some general commercial

uses of plant hormones

A

• Control of ripening
• Hormone rooting powders and
micropropagation
• Hormonal weedkillers

53
Q

List some specific uses of

plant hormones

A
• Auxins can be used in the 
productions of seedless fruit 
• Ethene is used to promote fruit 
dropping in plants e.g. cotton, 
walnuts and cherries 
• Cytokinins are used to prevent 
ageing of ripened fruit and 
products e.g. lettuces, and in 
micropropagation to control tissue 
development 
• Gibberellins can be used to delay 
ripening and ageing in fruit, to 
improve the size and shape of 
fruits, and in beer brewing to 
speed up the malting process
54
Q

How is ripening controlled by

plant hormones?

A
• The gaseous plant hormone 
ethene is involved in the ripening 
of climacteric fruits (i.e. fruits that 
continue to ripen after they have 
been harvested)
• Fruit is harvested when they are 
fully formed but long before they 
are ripe, because the unripe fruit is 
hard and less easily damaged in 
transport 
• When the fruit are needed for sale, 
they are exposed to ethene gas 
under controlled conditions to 
ensure each batch of fruit ripens at 
the same rate 
• Prevents a lot of wastage of fruit 
during transport, and increases the 
time available for them to be sold
55
Q

How are plant hormones used
in rooting powders and
micropropagation?

A
• Auxin affects the growth of both 
shoots and roots
• Applying auxin to cut shoots 
stimulates the production of roots, 
making it much easier to 
propagate new plants from 
cuttings 
• In both horticulture and 
agriculture, many plants are now 
propagated on a large scale by 
micropropagation, when 
thousands of new plants are 
grown from a few cells of the 
original plant
56
Q

How are plant hormones used

in hormonal weedkillers?

A
• The interactions between the 
different plant hormones is finely 
balanced to enable the plant to 
grow 
• Weeds interfere with crop plants, 
competing for light, space, water 
and minerals 
• Most weeds are broad-leaved 
dicots 
• If synthetic dicot auxins are 
applied as weedkiller, they are 
absorbed by the broad-leaved 
plants and affect their metabolism. 
The growth rate increase and 
becomes unsustainable, so they 
die 
• Synthetic auxins used are simple 
and cheap to produce, have very 
low toxicity to mammals, and are 
selective