Physiology of Gustation and Olfaction (Pierce) Flashcards
loss of sense of smell
anosmia
loss of sense of taste
aguesia
chemical compounds that bind taste receptors and impart the primary flavor categories (sweet, salty, bitter, sour, unami)
tastants
chemical compounds that bind odorant receptors and impart and odor
odorants
What is the structure of taste cells?
- taste bud cells are specialized epithelial cells
- apical domain (location of chemosensory transduction): contains microvilli, tastant receptors, voltage-gated ion channels, and TRP receptors
- basolateral domain (location of NT release): release of serotonin and ATP
How do tastants elicit an AP?
- ligand (tastant) binds receptor on apical domain
- receptor potentials of taste cells are usually depolarizing
- depolarization opens voltage-gated calcium channels and triggers transmitter release
- NT and mechanism of release differs by cell type
- NT binding to receptor on nearby primary sensory afferent terminal generates a receptor potential > action potential
What are the associated stimuli and NT’s for each tastant?
- sour: stim is H+ ions (acids), NT is Serotonin
- salty: stim is Na+ binding ENaC, NT is Serotonin
- sweet: stim is sugars binding GPCRs, NT is ATP
- unami: stim is glutamate binding mGluR4 (GPCR), NT is ATP
- bitter: stim is various compounds binding GPCRs, NT is ATP
How are odorants detected as smell?
- through odorant receptor neurons (ORNs) that are extremely sensitive to odorants
- olfactory cells are bipolar neurons that release glutamate as their NT
- ~12 million odors can be detected through ~350 odorant receptors: this diversity of smell occurs b/c one odorant can stimulate more than one type of OR which creates a unique combination or “signature” of that odorant
- also, conc of odorant matters (e.g. indole smells floral at low conc and putrid at high conc)
How do odorants elicit an AP?
- odorants diffuse into nasal mucus, bind receptor proteins on olfactory cilia, and activate olfactory cell
- olfactory receptor proteins are GPCR’s called Golf receptors
- uses second messenger transduction systems (cAMP)
- this opens cyclic-nucleotide gated channels (CNGC) allowing Na+ and Ca2+ to influx into cell
- depolarization occurs
- Ca2+-gated Cl- channels open that provides remainder of depolarization needed to generate appropriate receptor potential to achieve action potential
How do ORN’s adapt to a smell?
(when you “get used to” a smell and no longer notice its strength)
- receptor potential is reduced in magnitude when cAMP conc drops due to enzymatic break-down
- in addition, recovery depends on binding of calcium to calmodulin (Ca2+-CAM) reduces affinity of channel to cAMP, reducing cation influx
- odorant receptor itself can become phosphorylated, which modifies its sensitivity to odorants
What is the significance of the high-binding affinity of bitter tastants to their associated receptor?
- bitter taste = innately aversive: thought to guard against consuming poisons, many of which taste bitter to humans
- as a result, bitter-tuned GPCRs bind their ligand w/ very high binding affinity compared to other taste receptors
- evolved as a means to detect poisons at very low conc so to avoid additional ingestion of potentially toxic substance
- people can still learn to tolerate and seek out certain bitter and sour tastants, such as caffeine (bitter), green leafy vegetables (bitter), and citric acid (sour)
What is the physiological significance of tastants?
- sweet: signals presence of carbs that serve as energy source
- salty: governs intake of Na+ and other salts, essential for maintaining body’s water balance and blood circ
- umami: believed to reflect a food’s protein content due to presence of glutamate and few other AA’s
- sour: signals presence of dietary acids, b/c sour is generally aversive, we avoid ingesting excess acids and overloading mechanisms that maintain acid acid-base balance for body; also spoiled foods are often acidic thus are avoided
What are the causes of age related decrease in gustation/olfaction and what consequences do these changes have?
- gustatory: especially after 60, number of taste buds decrease, remaining taste buds shrink in size, mouth prod less saliva
- olfactory: esp after age 70, decreased nasal mucus prod, fibers and receptors of ORNs decrease
- exogenous causes: meds, diseases, smoking, pollutants, toxins in air
- consequences: adults add more salt/spices, can be problematic for older individuals w/ HTN or electrolyte/fluid problems; safe/effective salt sub has yet to be developed; loss of taste/smell results in appetite suppression and may lead to weight loss, malnutrition, impaired immunity, depression, and deterioration of medical conditions
What is the relationship between gustatory/olfactory development in utero?
- sense of smell/taste begin in utero
- primes growing fetus to taste amniotic fluid which reflects composition of maternal diet
- maternal diet represents external environment to which fetus would eventually be exposed
- primes rooting for breastmilk after birth through smell/taste sensitivity (looking for familiar tastes/smells, aka what mom was eating in utero)
How do newborns react to sweet tastants?
- respond in a pattern similar to pleasurable stimulation
- sweet tastes act as analgesics in infants and children during minor, painful procedures
- small amnts of sweet solution is placed on tongue of crying newborn to exert a rapid, calming effect along w/ decreased heart rate
- must be placed in oral cavity b/c direct stomach loading does not result in same outcome
- sucrose used to reduce procedural pain in infants w/ single painful events (heel lance, venipuncture, circumcision)