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Peripheral Blood Film Flashcards

1
Q

It is an important component in the evaluation of an ANEMIA.

It gives particular attention to RBC DIAMETER, SHAPE, COLOR, and INCLUSION.

It serves as a QUALITY CONTROL to verify results produced by AUTOMATED ANALYZERS.

A

Peripheral Blood Film Examination

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2
Q

A CAPSTONE OF A PANEL of tests in the peripheral film evaluation.

It includes an enumeration of cellular elements, quantitation of hemoglobin, and statistical analyses that provide a snapshot of CELL APPEARANCE.

A

Hemogram

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3
Q

Fill in the blanks:

Skin Puncture:

May be delivered using a ____ that is INSERTED through the RUBBER STOPPER of the EDTA TUBE.

A

Diff-Safe Dispenser

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4
Q

Multiple Choice:

What mm drop of blood is used in SKIN PUNCTURE?

A. 1-2 mm in diameter
B. 2-3 mm in diameter
C. 3-4 mm in diameter
D. 4-5 mm in diameter

A

B. 2-3 mm in diameter

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5
Q

What is the SIZE of the DROP OF BLOOD is IMPORTANT?

A

Thick Film: too large drop
Thin Film: too small drop

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6
Q

In how many HOURS does EDTA BLOOD DRAWING THE SPECIMEN?

A

2-3 hours

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7
Q

What ANTICOAGULANT is used for EDTA BLOOD?

A

Disodium
Tripotassium EDTA

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8
Q

What do you call when EDTA TUBES remain at room temperature for MORE THAN 5 HOURS?

A

Blood Cell Artifacts

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9
Q

In how many HOURS does EDTA BLOOD often have UNACCEPTABLE BLOOD CELL ARTIFACTS?

A

More than 5 hours

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10
Q

What are the Blood Cell Artifacts Types?

A

Echinocytes RBC’s
Spherocytes
Necrobiotic Leukocytes
Vacuolated Neutrophils

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11
Q

It is the BEST FOR EVALUATION OF BLOOD CELL MORPHOLOGY.

A

Anticoagulant-free Blood

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12
Q

What are the ADVANTAGES of ANTICOAGULANT-FREE BLOOD?

A
  • Made at the patient’s side
  • Some artifacts may be prevented
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13
Q

What are the DISADVANTAGES of ANTICOAGULANT-FREE BLOOD?

A
  • Platelet clumping
  • Few films can be made
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14
Q

PLATELETS AGGLUTINATES are similar in size to WBC.

A

EDTA Induce Platelet Clumping

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15
Q

What are the EFFECTS of EDTA BLOOD SMEAR (EDTA Induced Platelet Clumping)?

A
  • Pseudothrombocytopenia
  • Pseudoleukocytosis
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16
Q

In how many PERCENTAGE of SODIUM CITRATE did the sample recollect blood?

A

3.2%

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17
Q

What is the RECIPROCAL of the DILUTION FACTOR of the EDTA BLOOD SMEAR?

A

10/9 or 1.1

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18
Q

It is called PUSH because the SPREADER SLIDE IS PULLED INTO THE DROP OF BLOOD, and the film is made by pushing blood along the side.

A

Push-Type Wedge

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19
Q

In this procedure, the SPREADER SLIDE IS PUSHED INTO THE DROP OF BLOOD along the length of the slide to make the film.

A

Pulled Film

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20
Q

It is the EASIEST TO MASTER; MOST CONVENIENT; COMMONLY USED.

A

Manual Wedge Technique or Push-Wedge Method

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21
Q

What is the ANGLE EDGES of the blood smear?

A

30-45°

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22
Q

What is the SIZE OF THE INCH of the film slide?

A

3-inch x 1-inch

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23
Q

What is the SIZE OF THE LENGTH of the film slide?

A

75-mm x 25-mm

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24
Q

Fill in the blank:

Too high ——>

A

Thicker Smear

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25
Q

Fill in the blank:

Too low ——>

A

Thinner Smear

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26
Q

What is the SIZE DROP OF BLOOD in does TWO-GLASS SLIDE METHOD?

A

Large amount of blood - thicker
Small amount of blood - thinner

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27
Q

Fill in the blank:

Too fast ——>

A

Thicker Smear

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28
Q

Fill in the blank:

Too slow ——->

A

Thinner Smear

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29
Q

Fill in the blank:

Hematocrit of Patient;

Too high ——>

A

Angle is lowered as low as 25°

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30
Q

Fill in the blank:

Hematocrit of Patient;

Too low ——>

A

Angle is raised

31
Q

What is the LENGTH of the SLIDE FILM?

A

2/3 to 3/4

32
Q

What CHARACTERISTIC SHAPE of an IDEAL BLOOD SMEAR?

A

Finger-shaped
Tongue-shaped
Feathered-edged

33
Q

It may appear as a MOTH-EATEN LOOK to the RBCs and have heavily demarcated central pallor.

A

Water or Drying Artifacts

34
Q

In WATER or DRYING ARTIFACTS, how many PERCENTAGE of ANHYDROUS METHANOL before staining use?

A

10% volume-to-volume methanol

35
Q

It is RARELY USED; sometimes used for BONE MARROW aspirate smear preparation and EXCELLENT WBC distribution.

A

Cover Slip Technique

36
Q

What are the TWO METHODS of COVER SLIP TECHNIQUE?

A
  • Beacom’s Method
  • Ehrlich’s Method
37
Q

What are the TWO METHODS of AUTOMATED METHOD?

A

Miniprep
Centrifugal Type

38
Q

SEMI-AUTOMATIC portable instrument; stimulated MANUAL-WEDGE TECHNIQUE of blood smear preparation.

39
Q

It uses approximately 0.2mL of WELL-MIXED anticoagulant blood.

A

Centrifugal Type

40
Q

An AUTOMATED SLIDE-MAKING and STAINING SYSTEM.

A

Sysmex SP-10

41
Q

In Sysmex SP-10, how many SECONDS do films can produce?

A

Every 30 seconds

41
Q

Well Stained PBS:

MACROSCOPIC;

What is the color of the blood film?

A

Pink to purple

42
Q

Well Stained PBS

MICROSCOPIC;

What is the color of the RBC?

A

Orange to salmon pink

43
Q

Well Stained PBS

MICROSCOPIC;

What is the color of the WBC NUCLEI?

A

Purple to Blue

44
Q

Well Stained PBS

MICROSCOPIC;

What is the color of the CYTOPLASM OF NEUTROPHIL?

A

Pink to Tan with Violet or Lilac Granules

45
Q

Well Stained PBS

MICROSCOPIC;

What is the color of the EOSINOPHIL?

A

Bright Orange Refractile Granules

46
Q

It is for the evaluation of CELLULAR MORPHOLOGY.

A

Blood Film Staining

47
Q

What are the THREE SOLUTIONS of Blood Film Staining?

A
  1. Fixative: Methanol
  2. Stain: Romanowsky Stain
  3. Buffer: Age dist. H2O
48
Q

It is defined as ANY STAIN that contains METHYLENE BLUE and HALOGENATED FLUORESCEIN DYE

A

Romanowsky Stains

49
Q

A BASIC STAIN; it colors the nucleus and some cytoplasmic structures a BLUE or PURPLE COLOR.

A

Methylene Blue

50
Q

An ACIDIC STAIN; it colors some cytoplasmic structures an ORANGE-RED COLOR.

51
Q

What are the THREE EXAMPLES of ROMANOWSKY STAINS?

A
  1. Wright Stain
  2. Giemsa Stain
  3. May-Grunwald Stain
52
Q

This technique is DESIRABLE for STAINING PERIPHERAL BLOOD FILMS containing very high WBC counts, such as the films from LEUKEMIC PATIENTS.

This technique is best suited for LOW-VOLUME LABORATORIES.

A

Manual Technique

53
Q

In the Manual Technique, how many MINUTES does the stain SHOULD REMAIN ON THE SLIDE?

A

1-3 minutes

54
Q

In the Manual Technique, how many minutes does MIXTURE ALLOWED to remain on the slide?

55
Q

In this technique, slides may be automatically DIPPED IN STAIN and then in BUFFER and a series of rinses propelled along a platen surface by TWO CONVEYOR SPIRALS.

This technique is best suited for HIGH-VOLUME LABORATORIES.

A

Automated Slide Staining

56
Q

In Automated Slide Staining, how many MINUTES does STAIN A BATCH OF SLIDES?

A

5-10 minutes

57
Q

In Automated Slide Staining, how many HOURS in AQUEOUS SOLUTIONS of stain are stable?

58
Q

STAIN, BUFFER, and RINSE are pumped through holes in the platen surface, flooding the slide at the appropriate time.

A

Hema-Tek Device

59
Q

In the Hema-Tek Device, how many months are STAIN PACKAGES stable?

60
Q

This stain is a MODIFIED WRIGHT or WRIGHT-GIEMSA STAIN.

This technique is CONVENIENT and COST-EFFECTIVE for LOW-VOLUME LABORATORIES.

A

Quick Stains

61
Q

In the Quick Stains, how many minutes does take when STAIN ARE FAST AND EASY?

62
Q

Fill in the blanks:

Best staining results are obtained on ____ because the blood itself acts as a buffer in the staining process

A

Fresh Slides

63
Q

How many weeks does SLIDES STAINED after TURN-OUT TOO BLUE?

A

1 week or longer

64
Q

What are the THREE SCANNING METHODS?

A
  1. Cross-sectional or Crenellation
  2. Longitudinal
  3. Battlement
65
Q

It is used to ASSES DISTRIBUTION and the APPROPRIATE EVALUATION AREA FOR RBC.

A

Low Power Examination

66
Q

Red cells appear MACROCYTIC, FLATTENED, LACK CENTRAL PALLOR and WHITE BLOOD CELLS are OFTEN DISTORTED.

A

Feathered Edge

67
Q

Red cells appear MICROCYTIC and cells may seem to form rouleaux.

A

Thick Part

68
Q

It is used to ESTIMATE TOTAL WBC COUNT.

A

High Power Examination

69
Q

It is used to EXAMINE THE NUCLEAR DETAILS OF WHITE BLOOD CELLS.

It is also used for the TABULATION OF THE ACTUAL WBC DIFFERENTIAL and ESTIMATION OF PLATELET COUNT.

A

Oil Immersion Examination

70
Q

What are the PARASITES found in MALARIA?

A
  1. Plasmodium falciparum
  2. Plasmodium malariae
  3. Plasmodium ovale
  4. Plasmodium vivax
71
Q

What are the PARASITES found in FILARIA?

A
  1. Wucheria bancrofti
  2. Brugia malayi
  3. Loa loa
72
Q

What are the PARASITES found in TRYPANOSOMES?

A
  1. Trypanosama rhodesiense
  2. Trypanosama gambiense
  3. Trypanosama cruzi

PMTP 2 (14 decks)

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