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Peripheral Blood Film Flashcards

1
Q

It is an important component in the evaluation of an ANEMIA.

It gives particular attention to RBC DIAMETER, SHAPE, COLOR, and INCLUSION.

It serves as a QUALITY CONTROL to verify results produced by AUTOMATED ANALYZERS.

A

Peripheral Blood Film Examination

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2
Q

A CAPSTONE OF A PANEL of tests in the peripheral film evaluation.

It includes an enumeration of cellular elements, quantitation of hemoglobin, and statistical analyses that provide a snapshot of CELL APPEARANCE.

A

Hemogram

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3
Q

Fill in the blanks:

Skin Puncture:

May be delivered using a ____ that is INSERTED through the RUBBER STOPPER of the EDTA TUBE.

A

Diff-Safe Dispenser

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4
Q

Multiple Choice:

What mm drop of blood is used in SKIN PUNCTURE?

A. 1-2 mm in diameter
B. 2-3 mm in diameter
C. 3-4 mm in diameter
D. 4-5 mm in diameter

A

B. 2-3 mm in diameter

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5
Q

What is the SIZE of the DROP OF BLOOD is IMPORTANT?

A

Thick Film: too large drop
Thin Film: too small drop

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6
Q

In how many HOURS does EDTA BLOOD DRAWING THE SPECIMEN?

A

2-3 hours

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7
Q

What ANTICOAGULANT is used for EDTA BLOOD?

A

Disodium
Tripotassium EDTA

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8
Q

What do you call when EDTA TUBES remain at room temperature for MORE THAN 5 HOURS?

A

Blood Cell Artifacts

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9
Q

In how many HOURS does EDTA BLOOD often have UNACCEPTABLE BLOOD CELL ARTIFACTS?

A

More than 5 hours

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10
Q

What are the Blood Cell Artifacts Types?

A

Echinocytes RBC’s
Spherocytes
Necrobiotic Leukocytes
Vacuolated Neutrophils

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11
Q

It is the BEST FOR EVALUATION OF BLOOD CELL MORPHOLOGY.

A

Anticoagulant-free Blood

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12
Q

What are the ADVANTAGES of ANTICOAGULANT-FREE BLOOD?

A
  • Made at the patient’s side
  • Some artifacts may be prevented
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13
Q

What are the DISADVANTAGES of ANTICOAGULANT-FREE BLOOD?

A
  • Platelet clumping
  • Few films can be made
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14
Q

PLATELETS AGGLUTINATES are similar in size to WBC.

A

EDTA Induce Platelet Clumping

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15
Q

What are the EFFECTS of EDTA BLOOD SMEAR (EDTA Induced Platelet Clumping)?

A
  • Pseudothrombocytopenia
  • Pseudoleukocytosis
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16
Q

In how many PERCENTAGE of SODIUM CITRATE did the sample recollect blood?

A

3.2%

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17
Q

What is the RECIPROCAL of the DILUTION FACTOR of the EDTA BLOOD SMEAR?

A

10/9 or 1.1

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18
Q

It is called PUSH because the SPREADER SLIDE IS PULLED INTO THE DROP OF BLOOD, and the film is made by pushing blood along the side.

A

Push-Type Wedge

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19
Q

In this procedure, the SPREADER SLIDE IS PUSHED INTO THE DROP OF BLOOD along the length of the slide to make the film.

A

Pulled Film

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20
Q

It is the EASIEST TO MASTER; MOST CONVENIENT; COMMONLY USED.

A

Manual Wedge Technique or Push-Wedge Method

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21
Q

What is the ANGLE EDGES of the blood smear?

A

30-45°

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22
Q

What is the SIZE OF THE INCH of the film slide?

A

3-inch x 1-inch

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23
Q

What is the SIZE OF THE LENGTH of the film slide?

A

75-mm x 25-mm

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24
Q

Fill in the blank:

Too high ——>

A

Thicker Smear

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25
Fill in the blank: Too low ------>
Thinner Smear
26
What is the SIZE DROP OF BLOOD in does TWO-GLASS SLIDE METHOD?
Large amount of blood - thicker Small amount of blood - thinner
27
Fill in the blank: Too fast ------>
Thicker Smear
28
Fill in the blank: Too slow ------->
Thinner Smear
29
Fill in the blank: Hematocrit of Patient; Too high ------>
Angle is lowered as low as 25°
30
Fill in the blank: Hematocrit of Patient; Too low ------>
Angle is raised
31
What is the LENGTH of the SLIDE FILM?
2/3 to 3/4
32
What CHARACTERISTIC SHAPE of an IDEAL BLOOD SMEAR?
Finger-shaped Tongue-shaped Feathered-edged
33
It may appear as a MOTH-EATEN LOOK to the RBCs and have heavily demarcated central pallor.
Water or Drying Artifacts
34
In WATER or DRYING ARTIFACTS, how many PERCENTAGE of ANHYDROUS METHANOL before staining use?
10% volume-to-volume methanol
35
It is RARELY USED; sometimes used for BONE MARROW aspirate smear preparation and EXCELLENT WBC distribution.
Cover Slip Technique
36
What are the TWO METHODS of COVER SLIP TECHNIQUE?
- Beacom's Method - Ehrlich's Method
37
What are the TWO METHODS of AUTOMATED METHOD?
Miniprep Centrifugal Type
38
SEMI-AUTOMATIC portable instrument; stimulated MANUAL-WEDGE TECHNIQUE of blood smear preparation.
Miniprep
39
It uses approximately 0.2mL of WELL-MIXED anticoagulant blood.
Centrifugal Type
40
An AUTOMATED SLIDE-MAKING and STAINING SYSTEM.
Sysmex SP-10
41
In Sysmex SP-10, how many SECONDS do films can produce?
Every 30 seconds
41
Well Stained PBS: MACROSCOPIC; What is the color of the blood film?
Pink to purple
42
Well Stained PBS MICROSCOPIC; What is the color of the RBC?
Orange to salmon pink
43
Well Stained PBS MICROSCOPIC; What is the color of the WBC NUCLEI?
Purple to Blue
44
Well Stained PBS MICROSCOPIC; What is the color of the CYTOPLASM OF NEUTROPHIL?
Pink to Tan with Violet or Lilac Granules
45
Well Stained PBS MICROSCOPIC; What is the color of the EOSINOPHIL?
Bright Orange Refractile Granules
46
It is for the evaluation of CELLULAR MORPHOLOGY.
Blood Film Staining
47
What are the THREE SOLUTIONS of Blood Film Staining?
1. Fixative: Methanol 2. Stain: Romanowsky Stain 3. Buffer: Age dist. H2O
48
It is defined as ANY STAIN that contains METHYLENE BLUE and HALOGENATED FLUORESCEIN DYE
Romanowsky Stains
49
A BASIC STAIN; it colors the nucleus and some cytoplasmic structures a BLUE or PURPLE COLOR.
Methylene Blue
50
An ACIDIC STAIN; it colors some cytoplasmic structures an ORANGE-RED COLOR.
Eosin
51
What are the THREE EXAMPLES of ROMANOWSKY STAINS?
1. Wright Stain 2. Giemsa Stain 3. May-Grunwald Stain
52
This technique is DESIRABLE for STAINING PERIPHERAL BLOOD FILMS containing very high WBC counts, such as the films from LEUKEMIC PATIENTS. This technique is best suited for LOW-VOLUME LABORATORIES.
Manual Technique
53
In the Manual Technique, how many MINUTES does the stain SHOULD REMAIN ON THE SLIDE?
1-3 minutes
54
In the Manual Technique, how many minutes does MIXTURE ALLOWED to remain on the slide?
3 minutes
55
In this technique, slides may be automatically DIPPED IN STAIN and then in BUFFER and a series of rinses propelled along a platen surface by TWO CONVEYOR SPIRALS. This technique is best suited for HIGH-VOLUME LABORATORIES.
Automated Slide Staining
56
In Automated Slide Staining, how many MINUTES does STAIN A BATCH OF SLIDES?
5-10 minutes
57
In Automated Slide Staining, how many HOURS in AQUEOUS SOLUTIONS of stain are stable?
3-6 hours
58
STAIN, BUFFER, and RINSE are pumped through holes in the platen surface, flooding the slide at the appropriate time.
Hema-Tek Device
59
In the Hema-Tek Device, how many months are STAIN PACKAGES stable?
6 months
60
This stain is a MODIFIED WRIGHT or WRIGHT-GIEMSA STAIN. This technique is CONVENIENT and COST-EFFECTIVE for LOW-VOLUME LABORATORIES.
Quick Stains
61
In the Quick Stains, how many minutes does take when STAIN ARE FAST AND EASY?
1 minute
62
Fill in the blanks: Best staining results are obtained on ____ because the blood itself acts as a buffer in the staining process
Fresh Slides
63
How many weeks does SLIDES STAINED after TURN-OUT TOO BLUE?
1 week or longer
64
What are the THREE SCANNING METHODS?
1. Cross-sectional or Crenellation 2. Longitudinal 3. Battlement
65
It is used to ASSES DISTRIBUTION and the APPROPRIATE EVALUATION AREA FOR RBC.
Low Power Examination
66
Red cells appear MACROCYTIC, FLATTENED, LACK CENTRAL PALLOR and WHITE BLOOD CELLS are OFTEN DISTORTED.
Feathered Edge
67
Red cells appear MICROCYTIC and cells may seem to form rouleaux.
Thick Part
68
It is used to ESTIMATE TOTAL WBC COUNT.
High Power Examination
69
It is used to EXAMINE THE NUCLEAR DETAILS OF WHITE BLOOD CELLS. It is also used for the TABULATION OF THE ACTUAL WBC DIFFERENTIAL and ESTIMATION OF PLATELET COUNT.
Oil Immersion Examination
70
What are the PARASITES found in MALARIA?
1. Plasmodium falciparum 2. Plasmodium malariae 3. Plasmodium ovale 4. Plasmodium vivax
71
What are the PARASITES found in FILARIA?
1. Wucheria bancrofti 2. Brugia malayi 3. Loa loa
72
What are the PARASITES found in TRYPANOSOMES?
1. Trypanosama rhodesiense 2. Trypanosama gambiense 3. Trypanosama cruzi

PMTP 2 (14 decks)

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