Peripheral Blood Film Flashcards
It is an important component in the evaluation of an ANEMIA.
It gives particular attention to RBC DIAMETER, SHAPE, COLOR, and INCLUSION.
It serves as a QUALITY CONTROL to verify results produced by AUTOMATED ANALYZERS.
Peripheral Blood Film Examination
A CAPSTONE OF A PANEL of tests in the peripheral film evaluation.
It includes an enumeration of cellular elements, quantitation of hemoglobin, and statistical analyses that provide a snapshot of CELL APPEARANCE.
Hemogram
Fill in the blanks:
Skin Puncture:
May be delivered using a ____ that is INSERTED through the RUBBER STOPPER of the EDTA TUBE.
Diff-Safe Dispenser
Multiple Choice:
What mm drop of blood is used in SKIN PUNCTURE?
A. 1-2 mm in diameter
B. 2-3 mm in diameter
C. 3-4 mm in diameter
D. 4-5 mm in diameter
B. 2-3 mm in diameter
What is the SIZE of the DROP OF BLOOD is IMPORTANT?
Thick Film: too large drop
Thin Film: too small drop
In how many HOURS does EDTA BLOOD DRAWING THE SPECIMEN?
2-3 hours
What ANTICOAGULANT is used for EDTA BLOOD?
Disodium
Tripotassium EDTA
What do you call when EDTA TUBES remain at room temperature for MORE THAN 5 HOURS?
Blood Cell Artifacts
In how many HOURS does EDTA BLOOD often have UNACCEPTABLE BLOOD CELL ARTIFACTS?
More than 5 hours
What are the Blood Cell Artifacts Types?
Echinocytes RBC’s
Spherocytes
Necrobiotic Leukocytes
Vacuolated Neutrophils
It is the BEST FOR EVALUATION OF BLOOD CELL MORPHOLOGY.
Anticoagulant-free Blood
What are the ADVANTAGES of ANTICOAGULANT-FREE BLOOD?
- Made at the patient’s side
- Some artifacts may be prevented
What are the DISADVANTAGES of ANTICOAGULANT-FREE BLOOD?
- Platelet clumping
- Few films can be made
PLATELETS AGGLUTINATES are similar in size to WBC.
EDTA Induce Platelet Clumping
What are the EFFECTS of EDTA BLOOD SMEAR (EDTA Induced Platelet Clumping)?
- Pseudothrombocytopenia
- Pseudoleukocytosis
In how many PERCENTAGE of SODIUM CITRATE did the sample recollect blood?
3.2%
What is the RECIPROCAL of the DILUTION FACTOR of the EDTA BLOOD SMEAR?
10/9 or 1.1
It is called PUSH because the SPREADER SLIDE IS PULLED INTO THE DROP OF BLOOD, and the film is made by pushing blood along the side.
Push-Type Wedge
In this procedure, the SPREADER SLIDE IS PUSHED INTO THE DROP OF BLOOD along the length of the slide to make the film.
Pulled Film
It is the EASIEST TO MASTER; MOST CONVENIENT; COMMONLY USED.
Manual Wedge Technique or Push-Wedge Method
What is the ANGLE EDGES of the blood smear?
30-45°
What is the SIZE OF THE INCH of the film slide?
3-inch x 1-inch
What is the SIZE OF THE LENGTH of the film slide?
75-mm x 25-mm
Fill in the blank:
Too high ——>
Thicker Smear
Fill in the blank:
Too low ——>
Thinner Smear
What is the SIZE DROP OF BLOOD in does TWO-GLASS SLIDE METHOD?
Large amount of blood - thicker
Small amount of blood - thinner
Fill in the blank:
Too fast ——>
Thicker Smear
Fill in the blank:
Too slow ——->
Thinner Smear
Fill in the blank:
Hematocrit of Patient;
Too high ——>
Angle is lowered as low as 25°