Introduction to Urinalysis Flashcards
What is the normal urine color?
Yellow
It is a product of endogenous metabolism excreted by the body at a constant rate; responsible for the yellow color of urine.
Urochrome
Color of dilute urine?
Pale yellow
Color of concentrated urine?
Dark yellow
A pink pigment that attaches to urates; common in refrigerated urine.
Uroerythrin
Oxidation of urobilinogen causes an orange-brown color to unpreserved urine.
Urobilin
Color of bilirubin urine?
Dark yellow/Orange/Amber
It administered to UTI patients
Phenazopyridine or azo-Gantrisin
What is the RBC acidic urine color when hemoglobin oxidizes to methemoglobin?
Brown
It is due to skeletal muscle breakdown
Myoglobin
What does porphobilinogen oxidize?
Porphyrins
Red urine when clear can cause
- Hemoglobinuria (red plasma)
- Myoglobinuria (clear plasma)
Red urine when cloudy can cause
Hematuria (presence of red blood cells)
What is the color of urine in the presence of melanin or homogentisic acid?
Black color
What are the medications for urine that color black?
- Levodopa
- Methyldopa
- Phenol derivatives
- Metronidazole
What is the color of urine in the presence of pseudomonas infection?
Blue-green
What are the medications for urine that color blue-green?
- Methocarbamol
- Methylene blue
- Amitriptyline
What is the color of urine in the presence of indican?
Purple staining
Bacterial infection is due to?
Klebsiella or Providencia species
What is the normal clarity of urine?
Clear
Density; SG = 1.010
Isothenuric
Density; SG < 1.010
Hyposthenuric
Density; SG > 1.010
Hypersthenuric
It determines the concentration of dissolved particles by measuring the refractive index.
Refractometer
How many drops does the refractometer to urine?
1-2 drops
This technique used for the chemical analysis of urine
Reagent strip technique
What is the normal pH?
pH 4.5 to 8.0
What is the pH when slightly acidic?
pH 5.0 to 6.0
Red to yellow at pH 4.0 to 6.0?
Methyl red
Yellow to blue at pH 6.0 to 9.0?
Bromthymol blue
This pH parameter of the body is regulated by the kidneys and lungs through hydrogen secretion and reabsorption of bicarbonate from the filtrate in the convoluted tubules.
Acid-base content
It is often associated with early renal disease; and requires additional tests to determine if normal or pathologic.
Proteinuria
What is the normal protein range?
<10mg/dL or 100mg/24hr excreted
What are the protein error indicators?
- Tetrabromphenol blue
- Tetrachlorophenol
- Tetrabromosulfonphthalein
It is used for confirmatory tests for protein in urine.
Sulfosalycyclic acid
Most frequently performed due to diabetes detection and monitoring.
Glucose
One of the earliest methods of detecting glucose in the urine.
Copper reduction test
It employs Benedict’s test principle to detect glucose.
Clinitest tablet
This enzyme reaction colors green to brown
Potassium iodide
This enzyme reaction colors yellow to green
Tetramethylbenzidine
It is detected in the urine once fats are metabolized for energy due to the inability to metabolize carbohydrates.
Ketones
It detects Hematuria and Hemoglobinuria.
Blood
It is the appearance in the urine that can indicate bilirubinuria; also an early indication of liver disease.
Bilirubin
What principle reaction does bilirubin have?
A. Ehrlich reaction
B. Diazo reaction
C. Sodium nitroprusside reaction
D. Pseudoperoxidase reaction
B. Diazo reaction
What principle reaction does blood have?
A. Ehrlich reaction
B. Diazo reaction
C. Sodium nitroprusside reaction
D. Pseudoperoxidase reaction
D. Pseudoperoxidase reaction
What principle reaction does ketones have?
A. Ehrlich reaction
B. Diazo reaction
C. Sodium nitroprusside reaction
D. Pseudoperoxidase reaction
C. Sodium nitroprusside reaction
This product of reduction of bilirubin by intestinal bacteria once it is excreted into the intestine.
Urobilinogen
What principle reaction does urobilinogen have?
A. Ehrlich reaction
B. Diazo reaction
C. Sodium nitroprusside reaction
D. Pseudoperoxidase reaction
A. Ehrlich reaction
It is a rapid screening test for UTI; detection of bacteriuria to prevent serious complications.
Nitrite
What principle reaction does nitrite have?
Greiss reaction
What is the positive result of Nitrite?
Any shade of pink in the reagent strip
It is more standardized for leukocyte detection and is an indicator of UTI.
Leukocyte Esterase
What is the longest reading time of leukocyte esterase?
2 minutes
It minimizes external contamination.
Midstream clean-catch
What would be the specimen volume in preparation?
It should be 10-15mL
In how many minutes is the urine specimen centrifugal?
5 minutes