Path p. 222-223

Question 1

What does DNA laddering indicate?

Answer 1

indicator of apoptosis

Question 2

Name of nuclear fragmentation?

Answer 2

karyorrhexis

Question 3

Name of nuclear shrinkage?

Answer 3

pyknosis

Question 4

During karyorrhexis, —– cleave at internucleosomal regions, yielding fragments in multiples of — bp.

Answer 4

endonucleases

180

Question 5

T or F? Apoptosis requires ATP.

Answer 5

T

Question 6

T or F? Apoptosis is characterized by deeply eosinophilic cytoplasm, cell shrinkage, nuclear shrinkage and
basophilia, membrane tear and nuclear fragmentation.

Answer 6

F. membrane blebbing

Question 7

Radiation therapy causes —- of tumors and surrounding tissue via free radical formation and —– breakage.

Answer 7

apoptosis

dsDNA

Question 8

Which pathway is involved in tissue remodeling in embryogenesis?

Answer 8

Intrinsic pathway

Question 9

Extrinsic or intrinsic pathway? (FasL binding to Fas [CD95])

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Answer 9

Extrinsic

Question 10

Fas-FasL interaction is necessary in thymic

medullary —- selection.

Answer 10

negative

Question 11

Extrinsic or intrinsic pathway? Ratio change of anti : proapoptotic factors –>Ž  incr mitochondrial permeability and cytochrome c release.

Answer 11

intrinsic

Question 12

Extrinsic or intrinsic pathway? Immune cell (cytotoxic T-cell release of perforin and granzyme B)

Answer 12

Extrinsic

Question 13

T or F? BAX, BAK and Bcl-2 are proapoptotic proteins.

Answer 13

F. Bcl-2 is antiapoptotic.

Question 14

—- normally induces the activation of caspases in intrinsic ptw.

Answer 14

Apaf-1

Question 15

Pathology with overexpressed Bcl-2?

Answer 15

follicular lymphoma

Question 16

Defective —- interactions contribute to autoimmune disorders in extrinsic ptw.

Answer 16

Fas-FasL

Question 17

—- binds inactive caspases, activating them in Extrinsic ptw.

Answer 17

FADD

Question 18

T or F? In Liquifactive necrosis, proteins denature first, then enzymatic degradation takes place.

Answer 18

F. this happens in Coagulation necrosis

Question 19

Fragmented cells and debris surrounded by

lymphocytes and macrophages is seen in which type of necrosis?

Answer 19

Caseous

Question 20

Which necrosis shows incr cytoplasmic binding of acidophilic dyes?

Answer 20

Coagulative

Question 21

Saponification of fat in fat necrosis (combined with Ca2+) is stained with which dye and appear at what color?

Answer 21

appears dark blue on H&E stain

Question 22

Fibrinoid necrosis is due to?

Answer 22

Immune complexes combine with fibrin in vessels.

Question 23

Name 2 bacterial organisms that cause Caseous necrosis?

Answer 23

TB, Nocardia

Question 24

Which type fo necrosis causes enzymatic degradation first, then proteins denature?

Answer 24

Liquefactive

Question 25

Which necrosis cause vessel wall damage- -> appearing thick and pink?

Answer 25

Fibrinoid

Question 26

Name a necrosis that can be eithe Coagulative or Liquefactive?

Answer 26

Gangrenous

Question 27

Why liquifactive necrosis cause bacterial abscesses and brain infarcts?

Answer 27

due to incr fat content

Question 28

Name an ex. of Nonenzymatic fat necrosis?

Answer 28

breast trauma

Question 29

What is a liquifactive, gangrenous necrosis due to?

Answer 29

Wet: superinfection

Question 30

Name an ex. of enzymatic fat necrosis?

Answer 30

acute pancreatitis (saponification)