Past q from lab Flashcards
First step of Gr stain:
Crystal violet
Fixation of smear during Gr staining:
96% ethanol
Gr pos bacteria stain:
Purple
Gr neg bacteria stain
Pink
We can differentiate staphylococci from streptococci on the basis of oxidase production
False
Both negative
We can differentiate staphylococci from streptococci on the basis of catalase production
True
Staph: pos
Strep: neg
Equine strangles is caused by
Streptococcus equi ssp. equi
Morel disease of sheep is caused by
Staphylococcus aureus ssp. anaerobius
Staphylococcus can tolerate what NaCl concentration
up to 10%
Greasy pig disease is caused by
Staph hyicus
Staph aureus ssp anaerbius is catalase
Negative
Scarlet fever + Hu erysipelas is caused by
Strep pyogenes
Lactobacilli prefer pH
5.5-6.2
Staph aureus needs CO2 for capsule production?
False
No capsule
Lactobacilli is used for
Diary products
Strep is catalase/oxidase
Both neg
Which is more fastidious: strep or staph
Strep
E. rhusiopathiae found in
Su, Ov, Av, Hu
Listeria monocytogenes is catalase
Cat pos
E. rhusiopathiae morphology?
No thin rod/filament
L. monocytogenes is hemolytic bacterium
Beta-hemolytic
Nitrate ? Corynebacterium pseudotuberculosis strains can infect sheep/goats?
Nitrate negative
E. rhusiopathiae flagellated?
False
L. monocytogenes gives what in Bo?
Abortion
Rhodococcus equi found in
Soil
R colonies of E. rhusiopathiae forms ? in broth
Sediment
L. monocytogenes has flagella at what temp
22 C
R. equi produces any pigment?
Pink pigment
Corynebact pseudotuberculosis Nitrate negative biotype infects
Cap/Ov
Equine ulcerative lymphadenitis caused by
C. pseudotuberculosis
Su erysipelas is caused by
E. rhusiopathiae
Corynebacterium diphteria causes disease in
Humans: diphteria
E. rhusiopathiae causes what in birds?
Septicaemia
E. rhusiopathiae S colonies form what in broth
Turbidity
Selective medium for E. rhusiopathiae
NaN3, CV
R. equi capsule, pigment
Both (pink pigment)
Corynebacterium diphteria can produce
Exotoxins
Main diseases of Listeria
Abortion, encephalitis, septicaemia
Mycobacterium avium ssp. avium has what type of colonies on solid medium?
Cream-like
Mycobacterium are what kind of fast bactera?
Acid and alcohol fast
What staining can be used on mycobacterium?
Ziehl-Neelsen
What causes human leprosy
M. leprae
Give flagella status of mycobacteria
Most have no flagella
Dermatophilus are found where on inf animals
Skin
Nocardia asteroides produces what pigment
Carotenoid: orange ish
What causes Bo lumpy jaw
Actinomyces bovis
Actinobaculum suis can cause what in pigs
Pyelonephritis
Trueperella pyogenes can cause what in Bo
Arthritis, mastitis
What colour are Ziehl-Neelsen positive bacteria
Pink
Give air requirements of Mycobacteria:
Obligate aerobic
Mycobacterium bovis incubation time
4-8 weeks
Nocardia asteroides is found
In soil
Give air requirements of nocardia asteroides
Obligate aerobic
Actinomyces hordeovulneris can cause what in dogs
Pericarditis
Mycobacterium tuberculosis forms what on broth surface
Thin film
Mycobacterium bovis forms what on broth surface
Islands
Infectious form of what bacteria is a zoospore
Dermatophilus
Mycobacterium is catalase
Neg
Enterobacteriaceae can be found
GI tract
Genital tract
Resp tract
Environment
Enterobacteriacea are gram
Neg
Give air requirements of Enterobacteriaceae
Aerobic or facultative anaerobic
Enterobacteria are oxi
Neg
What enterobacteriaceae are non-motile
Shigella, Klebsiella
MacConkey agar contains what carbon source
Lactose
E.coli strains fimbrial antigens swine
F4, 6, 18
E.coli strains fimbrial antigens cattle
F5, 17, 41
E.coli can cause what in calves?
Septicaemia
Bismuth sulfide agar is used to isolate
Salmonella
E.coli strains isolated from calf can
hemolysis
E.coli strains isolated from swine can
form capsule
E.coli can cause edema disease in
Weaned piglets
E.coli is indole
Pos
Salmonella are fastidious or nah
Nah
Salmonella enteritidis is subspecies or species
Subspecies
S. cholerasuis gives what disease in pigs
Paratyphoid
Y. pseudotuberculosis produces flagella at what temp
22 C
Give air requirements for Fusobacteria
Obligate anaerobic
Foot rot of sheep is caused by
D. nodus
F. Necrophorum ssp necroforum
Salmonella is found where
Gut, sewage, water, food
Salmonella OF test
Fermentative
Human typhus is caused by
Rickettsia
Y. enterocolitica causes cross-reaction with
Brucella sp: O9 serogroups
–> cell wall
Serratia Marescens produces what at low temp?
Pigment: RED
Fusobacteria need what during culturing?
CO2
What causes necrobacillosis
Fusobacterium necrophorum ssp necrophorum
What salmonellas don’t have flagella
S. gallinarum, S. pullorum
Optimal temp for Y. pseudotuberculosis
30 C
Y. pseudotuberculosis does not affect
Ca, Fe
How many salmonella species are there
2
Salmonella Enterica and Salmonella Bongori
P multocida hemolysis?
Nope
P. pneumotropica can cause what in lab rodents
Pneumonia
B. trehalosi causes what in feedlot lambs
Acute systemic pasteurellosis
Shipping fever in cattle is caused by
Mannheimia haemolytica
What is the causative agent for contagious equine metritis (CEM)?
Taylorella equigenitalis
Su hemorrhagic necrotic pleuropneumonia is caused bu
Actinobacillus pleuropneumoniae
What is the causative agent of Glässer disease in piglets
Haemophilus parasuis
Histophilus somni needs what for culturing
CO2
Pasteurella members are found on what mucous membranes
Respiratory