Lab 5 Enterobacteriaceae: Escherichia, Klebsiella, Enterobacter Flashcards

1
Q

Enterobacteriaceae habitat

A

Gut: normal gut flora

Can get out into environment through feces

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2
Q

Enterobacteriaceae benefits

A

Most are saprophytes which can be beneficial for host:
Inhibits growth of unwanted pathogens
Produces vitamins
Help in digestion
Some contain enzymes which the host doesn’t have

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3
Q

Enterobacteriaceae morphology, resistance

A

Rod
Flagellated (except Shigella, Klebsiella)
Some have capsule and fimbria

Resistance: fair, moderate, average

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4
Q

Enterobacteriaceae culture

A

Simple
10-45
1 day to produce colony

Samples often contain a lot of bacteria and they can be hard to identify and separate from each other
Differentiation is mainly lactose fermentation

Selective enrichment: salmonella

Rambach agar: propylene glycol fermentation: salmonella
Chromocult coliform agar to diff btw coliform and e.coli
All contain lactose, indicator and inhibitory materials

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5
Q

Enterobacteriaceae low selectivity

A

Drigalski, MacConkey (bile salts inhibit swarming)

Methylene blue

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6
Q

Enterobacteriaceae medium selectivity

A

Phenol red: Escherichia and Salmonella

DC: Shigella

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7
Q

Enterobacteriaceae high selectivity

A

Bismuth sulfide agar: salmonella

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8
Q

Enterobacteriaceae biochemistry

A

Cat +
Oxi -
Ferm: acid + gas prod

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9
Q

Enterobacteriaceae antigens

A

O: cell wall
K: capsule
H: flagella (ø Shigella, Klebsiella)
F: fimbria (generally involved in pathogenicity)

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10
Q

Enterobacteriaceae grouping

A
Genus: biochem (Cat/Oxi, OF)
Species: biochem and serological
Serogroups: O antigens
Serotypes: O, K, H, F antigens
Biotypes: fermentation pattern
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11
Q

Enterobacteriaceae

Lactose positive

A

Escherichia
Klebsiella
Enterobacter
= EKE

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12
Q

Enterobacteriaceae

Lactose negative

A
Salmonella
Shigella
Yersinia
Proteus
Providencia
Edwardsiella
Serratia
Citrobacter

Yersinia tok Serr Salen på hestene sine Shigella og Edward, Pro, Pro, Citro

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13
Q

Enterobacteriaceae indol positive

A

E. coli

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14
Q

Enterobacteriaceae urease positive

A

Klebsiella

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15
Q

Enterobacteriaceae methyl red positive

A

E. coli

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16
Q

Enterobacteriaceae Voges-Proskauer positive

A

Klebsiella, Enterobacter

17
Q

Klebsiella habitat and morphology

A

Gut, resp tract, genital tract, mm, environment

Rod, capsule, no flagella

18
Q

Klebsiella culture

A

Large, merging, mucoid colonies because of capsule
Fast metabolism: rapid change of indicator colour
Serogroups differentiated based on capsule

19
Q

K. pneumoniae

A

Saprophytic

20
Q

Enterobacter habitat, morphology, colonies

A

Gut, environment

Rod, capsule, flagella

Mucoid colonies

21
Q

Enterobacter pathogenicity

A

Saprophytic
Rarely resp infection in Hu
E. cloacae, E. aerogenes