Lab 10 Vibrio, Campylobacter, Lawsonia, Helicobacter, Arcobacter, Spirochaetes Flashcards
Vibrio habitat, morphology, staining
Water, brackish and high salt content (halophilic)
Intestine of aquatic animals
Curved rod, polar flagella
Gr-
Vibrio culture, biochemistry
Not fastidious, optimal temp lower than 37
Fac. anaerobic
Selective culture: pH 8.6
Cat +
Oxi +
OF: F
V. cholerae
Hu cholera: ø clean water
= enteric infection, vomiting and extreme watery diarrhea
Fluid loss up to 20l/day
155 O-groups
NAG: non-cholera vibrios: fish, birds diarrhea
V. metschnikovii
Sewage, aquatic animals
OXI NEG
Poultry hemorrhagic enteritis
V. parahaemolyticus
ZOONOSIS
Sea animals: crab, shellfish
Thermostabile haemolysin
Food-borne inf in Hu: Gastroenteritis, fever, septicaemia
Ass. with consumption of undercooked seafood
Listonella (V.) anguillarum
Marine fish (salmon, eel) skin lesions, septicaemia
Lawsonia habitat, morphology, staining and culture
Swine enterocytes, obligate intracellular!
Curved thin rods
Gr-
Microaerophilic, enterocyte cell culture
L. intracellularis
Swine proliferative enteropathy (ileum, colon, caecum)
Thickening of int wall, necrosis of mm, enlargement of mesenterial ln
Helicobacter habitat, morphology, resistance, staining
Carnivores mucous membranes Hu stomach and duodenum Curved helical rod with flagella R: weak Gr-
Helicobacter culture, biochemistry
Microaerophilic, fastidious: blood, Aa
Cat + Oxi + Urease + (urease prod ammonia: local alkaline, can survive --> gastric ulcer) Not splitted CH, cytotoxin (H.pylori)
H. pylori
Hu chronic gastritis, gastric/duodenal ulcer
H. felis
Ca, Fe
H. canis
Ca
H. mustelae
H. acinonychis
H. bizzozeronii:
Weasel
Cheetah
Su gastric ulcer, Ca, Fe
Arcobacter habitat, morphology, staining, culture
Mm: genitals, intestine Curved rods, flagella Gr- Microaerophilic --> aerotolerant Leptospira and Campylobacter media
Arcobacter
Cryaerophilus
Skirrowii
Butzleri
Bo, Su: abortion, mastitis
Bo, Su, Ov: abortion, prepuce
Hu, Apes: enteritis, Bo: abortion
Campylobacter habitat, morphology, resistance, staining
Curved rods in gull-winged shapes and spiral forms
Polar flagella
Resistance: weak
Gr-
Campylobacter culture
Fastidious
Blood agar w/ pyruvate
Microaerophilic: 6% O2, 10% CO2, 84% N2
Selective culture: vancomycin, polymyxin, trimethoprim
Semisolid media
Campylobacter biochemistry
Cat + (except C. mucosalis, C. sputorum)
Oxi +
No CH splitting
Species, subspecies can be diff based on biochemical characteristics