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Bacteriology > Lab 10 Vibrio, Campylobacter, Lawsonia, Helicobacter, Arcobacter, Spirochaetes > Flashcards

Lab 10 Vibrio, Campylobacter, Lawsonia, Helicobacter, Arcobacter, Spirochaetes Flashcards

1
Q

Vibrio habitat, morphology, staining

A

Water, brackish and high salt content (halophilic)
Intestine of aquatic animals

Curved rod, polar flagella

Gr-

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2
Q

Vibrio culture, biochemistry

A

Not fastidious, optimal temp lower than 37
Fac. anaerobic
Selective culture: pH 8.6

Cat +
Oxi +
OF: F

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3
Q

V. cholerae

A

Hu cholera: ø clean water
= enteric infection, vomiting and extreme watery diarrhea
Fluid loss up to 20l/day
155 O-groups
NAG: non-cholera vibrios: fish, birds diarrhea

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4
Q

V. metschnikovii

A

Sewage, aquatic animals
OXI NEG
Poultry hemorrhagic enteritis

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5
Q

V. parahaemolyticus

A

ZOONOSIS
Sea animals: crab, shellfish
Thermostabile haemolysin
Food-borne inf in Hu: Gastroenteritis, fever, septicaemia
Ass. with consumption of undercooked seafood

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6
Q

Listonella (V.) anguillarum

A

Marine fish (salmon, eel) skin lesions, septicaemia

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7
Q

Lawsonia habitat, morphology, staining and culture

A

Swine enterocytes, obligate intracellular!
Curved thin rods
Gr-

Microaerophilic, enterocyte cell culture

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8
Q

L. intracellularis

A

Swine proliferative enteropathy (ileum, colon, caecum)

Thickening of int wall, necrosis of mm, enlargement of mesenterial ln

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9
Q

Helicobacter habitat, morphology, resistance, staining

A 
Carnivores mucous membranes
Hu stomach and duodenum
Curved helical rod with flagella
R: weak
Gr-
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10
Q

Helicobacter culture, biochemistry

A

Microaerophilic, fastidious: blood, Aa

Cat +
Oxi +
Urease +
(urease prod ammonia: local alkaline, can survive --> gastric ulcer)
Not splitted CH, cytotoxin (H.pylori)
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11
Q

H. pylori

A

Hu chronic gastritis, gastric/duodenal ulcer

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12
Q

H. felis

A

Ca, Fe

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13
Q

H. canis

A

Ca

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14
Q

H. mustelae
H. acinonychis
H. bizzozeronii:

A

Weasel
Cheetah
Su gastric ulcer, Ca, Fe

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15
Q

Arcobacter habitat, morphology, staining, culture

A 
Mm: genitals, intestine
Curved rods, flagella
Gr-
Microaerophilic --> aerotolerant
Leptospira and Campylobacter media
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16
Q

Arcobacter
Cryaerophilus
Skirrowii
Butzleri

A

Bo, Su: abortion, mastitis
Bo, Su, Ov: abortion, prepuce
Hu, Apes: enteritis, Bo: abortion

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17
Q

Campylobacter habitat, morphology, resistance, staining

A

Curved rods in gull-winged shapes and spiral forms
Polar flagella

Resistance: weak

Gr-

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18
Q

Campylobacter culture

A

Fastidious
Blood agar w/ pyruvate
Microaerophilic: 6% O2, 10% CO2, 84% N2

Selective culture: vancomycin, polymyxin, trimethoprim
Semisolid media

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19
Q

Campylobacter biochemistry

A

Cat + (except C. mucosalis, C. sputorum)
Oxi +
No CH splitting
Species, subspecies can be diff based on biochemical characteristics

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20
Q

C. fetus ssp. fetus

A

Ov genital campylobacteriosis: abortion

Mammals and birds: enteritis

21
Q

C. fetus ssp. venerealis

A

Bo genital campylobacteriosis
Bulls asymptomatic carriers: infect cows
Abortion, infertility

22
Q

C. jejuni

A

Gut of mammals and birds
Ru abortion, mastitis
Ca/Hu: intestinal campylobacteriosis
Av campylobacter hepatitis

23
Q

C. lari

A

Gut of wild birds and dog, enteritis

24
Q

C. coli

A

Su/Hu: enterocolitis

25
Q

C. mucosalis, C. hyointestinalis

A

Su gut, saprophytes

CAT NEG

26
Q

Spirochaetas:

A

Borrelia, Treponema, Brachyspira, Leptospira

27
Q

Spirochaetas habitat, morphology, resistance

A

Mucous membranes
Arthropods
Environment

Thin, helical filaments, NO FLAGELLA

Special structure:
Periplastic flagella: axial movement
Elastic cell membrane

28
Q

Spirochaetas staining

A

Gr-, staining can be difficult
Unstained: dark field/phase contrast microscope

Giemsa: methanol, methylene blue

India ink: background
Levaditi’s silver impregnation

Most are OBLIGATE ANAEROBe

29
Q

What Spirochaetas are difficult to culture?

A

Borrelia, Treponema: animal trial

30
Q

Which require special demands but can be cultured?

A

Brachyspira, Leptospira

31
Q

Borrelia habitat, morphology

A

Infected animals, rodents, arthropods (TICKS)
Tropical countries
15-20 periplastic flagella
Irregular, linear chromosome

32
Q

B. anserina

A

Avian borreliosis: avian spirochaetosis
Staining: fuchsin, Giemsa, Levaditi, unstained
Culture: fastidious (Pr, Hgb), egg, animal trial
R: weak
Vectors: ticks, acari

33
Q

B. burgdorferi, B. afzelii, B. garinii

A 
LYME-BORRELIOSIS
Staining: Giemsa or unstained
Difficult to culture
Natural hosts: small rodents, ticks (vectors)
Hu, Ca, Eq: Lyme disease (JB)
34
Q

B. thelieri

A

Eq, Bo borreliosis in tropics

35
Q

B. recurrentis, B. hispanica, B. caucasica

A

Hu tick-borne relapsing fever

36
Q

Treponema habitat, staining

A

Infected animals, humans, mucous membranes (oral cav, genitals)
Pathogenic species cannot be stained or cultured

37
Q

T. pallidum
T. paraluiscuniculi
T. denticola, T. parvum

A

Hu syphilis
Rabbit syphilis
Hu oral cav

38
Q

Brachyspira habitat, morphology, staining, culture

A

Swine large int (old name serpulina)

Helical rods

Fuchsin, Giemsa, unstained
Histo –> silver!

Obligate anaerobic!
Fastidious: blood agar (beta/weak hemolysis)
Smear from feces: spiral

39
Q

B. hyodysenteriae

A 
fast, obl ana, blood agar, 3-7 days, beta hemolysis
Selective: spectinomycin
Ag: 9 serogroups
R: weak, can live 1 week in feces
Swine dysentery!
40
Q

B. pilosicoli

A

Weak hemolysis

Su int spirochaetosis

41
Q

B. intermedia

A

Weak hemolysis

Su spirochaeta colitis

42
Q

B. innocens

A

Saprophyte, weak hemolysis

43
Q

Leptospira habitat, morphology, resistance

A

Inf animals: pathogens
Environment, water: saprophytes
Helical rods, hooked

R: weak
Dry environment: hours
Water: weeks

44
Q

Leptospira staining, culture

A 
Unstained or levaditis silver impregnation
Aerobic, 28-30C
Primary culture: 6-16 weeks
Pure culture: 1 week
Nutrients: FFA, inorganic N, Vit B1, B12
Korthof medium, EMJH medium
Animal trial
45
Q

Leptospira biochem, antigens

A

EC lipase production is characteristic
21 species
7 are pathogenic

46
Q

L. ichterohaemorrhagiae

A

Ca, Eq generalized disease, fever

47
Q 
Leptospirosis
L. ichterophaemorrhagiae
L. grippotyphosa
L. hardjo
L. sejroe
L. pomona
L. tarassovi
L. canicola
A

Kidney, liver

Abortion, fever, hemoglobinuria

48
Q

L. hardjo

A

Bo, Ov abortion

49
Q

L. pomona

A

Su, Bo abortion, hemoglobinuria

Bacteriology (13 decks)

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