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Bacteriology > Lab 11 Mycoplasma, Rickettsia, Chlamydia > Flashcards

Lab 11 Mycoplasma, Rickettsia, Chlamydia Flashcards

1
Q

Rickettsiaceae genera and their propagation

A

Rickettsia, Coxiella: propagate in all cell types
Ehrlichia, Neorickettsia: lymphoid cells, endothel cells
Anaplasma: in or on erythrocytes, in lymphoid cells

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2
Q

Rickettsiaceae habitat, morphology and staining

A

Obligate CELL PARASITES
Arthropod vectors
Small rodents

Tiny coccoid rods, non-motile
Staining difficult
Giemsa
Köster
Stamp
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3
Q

Rickettsiaceae culture and classification

A

Living cells
Embryonated eggs
Cell culture
Laboratory animals

Cell tropism, intra/extracellular propagation, vector

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4
Q

Rickettsia habitat

A

Reservoir: rodents and arthropods

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5
Q

R. prowazekii + vector

A

Epidemic typhus fever

Louse

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6
Q

R. rickettsii + vector

A

Rocky mountain spotted fever

Ticks

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7
Q

R. sibirica + vector

A

North-Asian tick-borne rickettsiosis

Ticks

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8
Q

R. conorii + vector

A

Boutouneuse fever

Ticks

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9
Q

Coxiella

A

Very good resistance (50 days in dust)

Inoculate into embryonated eggs

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10
Q

C. burnettii

A

COXIELLA
worldwide, no vectors are needed (ticks)
Hu, Bo, Ov, Cap: Q-fever

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11
Q

Ehrlichia, Neorickettsia

A

Coccoid

Tissue cultures, cannot be cultured in embryonated eggs

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12
Q

E. canis

A

Ca monocytic ehrlichiosis (generalized, fever)

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13
Q

E. ruminantium

A

Ru heartwater

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14
Q

Neorickettsia risticii

A

Potomac horse fever

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15
Q

Anaplasma

A

Ticks, ruminants

No culturing in vitro (no cell wall)

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16
Q

A. marginale

A 
Bo anaplasmosis (anemia)
Tropical and subtropical countries
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17
Q

A. ovis

A

Cap, Ov anaplasmosis (anemia)

18
Q

A. phagocytophilum

A

Tick-borne fever of Ru

Eq granulocytic ehrlichiosis: edema, lymphadenopathy

19
Q

Chlamydia habitat and replication

A

Mucous membranes of gut, genitals and resp tract
Persistent - subclinical infection

Replication
Exists in two morphologically distinct forms:
1. Small infectious elementary body
Like a spore, resistant to harsh environmental factors

  1. Larger, non-infectious reticulate body
20
Q

Chlamydia morphology and resistance

Genetic material, where does it propagate, what does it need a cell for?

A

General features: resembles virus
Has both RNA and DNA
Replication with binary fission, cell wall, metabolic enzymes
Susceptible to antibiotics, obligate intracellular
Propagate in the vacuoles of the cytoplasma of the host cell
Unique propagation cycle
Elementary body –> reticulate body
Unable to produce ATP: energy parasites

No peptidoglycan layer from cell wall but genus specific LPS

R: weak, survives in feces, fetal membranes and dust for 1-3 weeks

21
Q

Chlamydia staining

A

Gr-

Stamp staining:
Fixation
Fuchsin
Acetic acid
Malachite green

Giemsa

22
Q

Chlamydia culture and antigens

A 
Living cells
5-7 day old embryonated egg
Tissue culture
Animals
Replication in vacuoles of cells

Genus specific LPS: heat resistant
Species specific protein

23
Q

Chlamydophila psittaci

A

Av psittacosis
Parrot disease, fever
Lots of mammals and Hu

24
Q

Chl. trachomatis

A

Hu: trachoma, chronic purulent conjunctivitis

Lymphogranuloma venereum: infl of urethra, vaginitis, enlargement of ln

25
Q

Chl. abortus

A

Ov, Cap, Bo, Su: abortion

26
Q

Chl. felis

A

Fe conjunctivitis

27
Q

Chl. pecorum

A

Ru arthritis, pneumonia, encephalomyelitis

28
Q

Chl. pneumoniae

A

Hu pneumonia

29
Q

Mycoplasma habitat

Eperythrozoon, Haemobartonella

A

Mucous membranes (resp, alimentary, genital tract) of Hu and animals like rodents, Ru, Su, arthropods
On ERYTHROCYTES
plants

30
Q

Mycoplasma morphology, resistance, staining

A 
Smallest bacteria
Pleomorphic (NO CELL WALL)
Pear shaped
Long branching filaments
R: weak

Gr-
Giemsa
No cell wall

31
Q

Mycoplasma culture

A 
Fastidious, horse serum (sterols, FAs)
Yeast extract, DNA, lactalbumin hydrolysate, urea, cysteine
No turbidity
Typical colony takes 7-14 days to form
CO2 need
Selective isolation
Penicillin
Tallium acetate
Some cannot be cultured
(Suis, ovis, haemocanis, haemofelis)
32
Q

Mycoplasma biochemistry, antigens, classification

A

Glu fermentation, Arg hydrolysis, cholesterol demand

Ag
Growth inhibition test
Metabolism inhibition test
Surface proteins

Classification
Family: genome size, cholesterol demand, morphology
Genus: optimal temp, pH, glu, arg, urease

33
Q

Human mycoplasma

A

M. pneumoniae atypical pneumonia

34
Q

Bo mycoplasma (3, pneumonia)

A

M. mycoides ssp mycoides: cont. bovine pleuropneumonia
(Notifiable)
M. bovigenitalium: pneumonia, genitals
M. bovis: arthritis, mastitis, pneumonia

M. dispar: calf pneumonia

M. bovoculi: kerato-conjunctivitis

M. californicum/M. canadense: mastitis

35
Q

Ov/Cap mycoplasma

A

M. capricolum ssp. capripneumoniae: cont pleuropneumonia

M. capricolum ssp capricolum: pneumonia, arthritis, septicaemia

M. mycoides ssp capri

M. agalactiae: pneumonia, contageous agalactica, arthritis

M. conjunctivae: kerato-conjunctivitis

M. ovipneumoniae

M. ovis: anemia

36
Q

Su mycoplasma

A

M. suis: Su eperythrozoonosis (anemia)

M. hyopneumoniae: enzootic pneumonia

M. hyorhinis: polyserositis, rhinitis, arthritis

M. hyosynoviae: arthritis

37
Q

Car mycoplasma

A

M. haemofelis
M. haemocanis
Anaemia, haemobartonellosis

M. felis: conjunctivitis

M. cynos: resp inf of Ca

38
Q

Av mycoplasma

A

M. gallisepticum: CRD

M. synoviae: synovitis turkey

M. meleagridis, M. iowae, M. anatis, M. aseris: air sacculutus

39
Q

Ureaplasma

A

CO2, cholesterol
Urease +

U. urealyticum: hu genital inf

U. diversum: Bo genital inf

U. canigenitalium

40
Q

Acholeplasma

A

No cholesterol need

A. axanthum
A. oculi sheep conjunctivitis

Bacteriology (13 decks)

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