Lab 4 Mycobacterium etc Flashcards
Mycobacterium habitat
Infected host: obligate pathogens
Environment: facultative pathogens
Soil, water, plants
Mycobacterium morphology and resistance
Morpholoy: rods, coccoid, filaments
Mycobacterium cell wall structure
Mycolic acids
Lipids, waxes (increases resistance)
Surface proteins: virulence factors
Mycobacterium stains:
Gram +
Ziehl-Neelson positive (red)
- Fuchsin binds to lipids
Acid and alkaline fast
Mycobacterium culture:
Obligate aerobic (needs oxygen) Broth: Henley, Sauton (cord formation) Solid: egg, serum agar, glycine potato Selective: petragnani (malachite green) Inoculation after acid or alkaline treatment
What is different with M. avium subsp. paratuberculosis?
Needs mycobactin in the culture
M. tuberculosis Incubation time Shape Solid medium Broth
3-6 weeks
Slender rods
Dry colonies
Thin film
M. bovis Incubation time Shape Solid medium Broth
4-8 weeks
Short rods
Wet colonies
Islands
M. avium subsp. avium Incubation time Shape Solid medium Broth
2-3 weeks
Polymorph
Cream-like colonies
Thick film, sediment
M. avium subsp. paratuberculosis
6-12 weeks
Short rods
Wet colonies
Thin film
Mycobacterium antigens
Glycolipids, lipoproteins
Cross-reactions within genera (BCG)
Immunoelectrophoresis
Tuberculin, uses
Old/New
Used to test for tuberculosis (Mantoux)
Old: broth culture, boiled, filtered, conc
New: purified protein derivate (trichloroacetic acid
Mycobacterium Obligate pathogens
Tuberculosis, paratuberculosis
Mycobacterium Facultative pathogens
Mycobacteriosis, atypical
3 groups/complexes of mycobacterium
M. tuberculosis
M. avium
M. leprae
M. tuberculosis-complex
M. tuberculosis M. africanum M. bovis M. caprae M. microti M. pinnipedi
M. tuberculosis
Hu Su Ca Fe Bo Parrot
M. africanum
Hu
Bo
Su
M. bovis
Hu Ru Su Ca Fe BCG vax came from a M.bovis strain
M. caprae
Hu Ru Su Ca Fe
M. microti
Rodents
M. pinnipedi
Seals
Pathogenic mycobacteria
M. avium subspecies: Avium Paratuberculosis Silvaticum Hominissuis
M. avium subsp. avium
Bird tuberculosis
M. avium subsp. paratuberculosis
Ruminant paratuberculosis (small int)
M. avium subsp. silvaticum
Bird tuberculosis, ruminant paratuberculosis
M. avium subsp. hominissuis
Su, Hu
M. leprae
Human leprosy (spedalskhet)