Lab 9 Brucella, Pseudomonas, Burkholderia, Aeromonas, Francisella, Bordetella, Moraxella

Question 1

Francisella habitat and morphology, resistance

Answer 1

Rodents, arthropods, environment

Coccoid rods, thin capsule

R: weak but good survival in cold

Round thin rats are weak but survive the cold in sanFRAN

Question 2

Francisella stain, culture

Answer 2

Gr-, bipolar
Aerobic, fastidious
Additives: protein, Cys, Yeast extract

Francis-Blood agar
Cys-Glu-Rabbit serum/egg

Question 3

Francisella biochemistry

Answer 3

Cat +, Oxi -
Glycerol
Pos: F. tularensis ssp tularensis and mediasiatica
Neg: F. tularensis ssp holarctica

Question 4

Francisella antigens, cross reactions with?

Answer 4

Uniform

Cross reactions with brucella and Y. pestis

Question 5

F. tularensis ssp. tularensis and ssp holarctica

Answer 5

Rodents, Ca, Fe, Bo, Ov, Su, Hu: tularaemia

Question 6

Bordetella habitat, morphology and stain, culture

Answer 6

Resp mm of mammals and birds (alle puster bordet)

Rods

Gr-

Obligate aerobic !!
Needs air, is found in resp mm!

Question 7

Bordetella biochemistry

Answer 7

Cat +
Oxi +!
Cannot utilize CH

Question 8

Bord. pertussis

Answer 8

Human whopping cough (kikhoste)
DIPERTE: vax
Diphteria (Coryne)
Pertussis (Bordetella)
Tetanus (Clostridium)

Question 9

Bord. parapertussis

Answer 9

Hu parapertussis

Question 10

Bord. bronchiseptica

Answer 10

Fimbria, growth on media containing only CITRATE
Selective isolation: CV, bile salts, penic, nitrofurantoin
Weak resistance
Su atrophic rhinitis (w/ P. multocida)
Ra pneumonia
Ca distemper complication
Fe bronchitis, bronchopneumonia

Question 11

Bord. avium

Answer 11

Poultry resp tract
Capsule, flagella, fimbria
Simple culture
Resp disease of turkey, a lot like Coryza

Question 12

Moraxella habitat, morphology, resistance

Answer 12

Mucous membrane of upper resp tract, conjunctiva and genitals

Coccoid rods, capsule, in pairs

R: weak

Question 13

Moraxella staining, biochemistry

Answer 13

Gr -

Cat +
Oxi +

Question 14

Mor. lacunata

Answer 14

Hu kerato-conjunctivitis

Question 15

Mor. bovis

Answer 15

Rods in pairs, fimbria
Fastidious culture, blood agar w/ beta hemolysis
Proteases, hyaluronidase, fibrinolysin
Infectious kerato-conjunctivitis of Bo: PINK EYE

Question 16

What causes pink eye of Bo:

Answer 16

Moraxella bovis

Question 17

Mor. ovis

Answer 17

Ov, calf kerato-conjunctivitis, nasal cavity

Cocci in pairs

Question 18

Brucella habitat, morphology, resistance

Answer 18

Host body, mucous membrane of genitals
Can’t survive in environment
Monospecies system/Multispecies system
B. melitensis abortus 1, 2, 3 / B. abortus 1, 2, 3

Coccoid rods, facultative IC
R: fair

Question 19

Brucella staining and culture

Answer 19

Gr-
Köster staining (safranin, H2SO4, methylene blue) A type of modified Ziehl-Neelsen

Obligate aerobic: NEEDS OXYGEN
Fastidious
Requires CO2 (10-15%) - brus har kullsyre
S-R colonies

Selective culture: polymyxin, bacitracin, cyclohexamide
Lab animals

Question 20

Brucella biochemistry, antigens

Answer 20

Cat +
Oxi +
UREASE POS

Ag:
S type: B. melitensis, B. abortus, B. suis cross reaction
R type: B. ovis, B. canis cross reaction
Cell wall A and M polysacch: mel, abo, suis

Differentiation of species:
Phage susceptibility, oxidative metabolism, natural host
Biotype differentiation:
CO2 demand, H2S, Fuchsin/thionin tolerance, agglutination

Question 21

Bruc. melitensis

Answer 21

Ov/Cap, testicle dilation (orchitis) dangerous!!

Notifiable

Question 22

Bruc. abortus

Answer 22

Bo

Question 23

Bruc. suis

Answer 23

Su, Hare, reindeer, rodents

Question 24

Bruc. pinnipediales
B. ceti
B. ovis
B. canis
B. neotomae
B. microti

Answer 24

Seal, walrus
Whale, dolphin
Ov
Ca
Rat
Vole

Question 25

Pseudomonas/Burkholderia

Habitat, morphology, resistance

Answer 25

Soil, water, plants, hospital environments (Except B.mallei)

Flagellated rods (except B. mallei)
Many factors, enzymes and toxins that enhance virulence and make them resistant to commonly used Abs

Question 26

Pseudomonas/Burkholderia

Staining, culture

Answer 26

Not fastidious
Obligate aerobic: NEEDS OXYGEN
Pigment production
Glycerol

Question 27

Burk. mallei

Answer 27

GLANDERS
No flagella, irregular staining, no pigment production
Weak resistance, Ew, Fe, Hu malleus

Question 28

Burk. pseudomallei

Answer 28

Soil, water, tropics
Flagella! Glycerol agar, large colonies, forms layer in broth
All mammals incl Hu excl Eq: melioidosis

Question 29

Pseudomonas aeruginosa

Characteristics

Answer 29

Higher resistance, can live everywhere (water, sewage, gut)
1 polar flagellum, polar fimbria
Simple culture, 5-42C, beta hemolysis, odor and pigments
–> GREEN COLOUR

Susceptible: dehydration, heat or disinfection
Resistant: quaternary NH4 salts, antibiotics

Question 30

Pseudomonas aeruginosa

Pathogenicity

Answer 30

Facultative pathogenic, local suppuration
Bo, Eq: mastitis, metritis
Ov: dermatitis
Ca: otitis externa
Fur animals: septicaemia
Hu: nail and oral infection

Question 31

Pseudomonas anguilliseptica

Answer 31

Inactive biochemistry

Salmon, eel: septicaemia, keratitits, hemorrhages

Question 32

Aeromonas habitat, morphology, culture, groups

Answer 32

Water, sewage, gut
Rods, flagellated
Simple culture, aerobic/facultative anaerobic

Groups:
Mesophilic: A. hydrophila
= moderate temp
Psychrophilic: A. salmonicida
= cold temps

Question 33

Aero. hydrophila

Answer 33

Hemolysis, optimal temp 28
Resembles E.coli: sometimes gas is prod
Colonise farm animals
Snake, frog, fish: septicaemia

Question 34

Aero. salmonicida

Answer 34

Small coccobacillus, NO FLAGELLA
20C, dark brown pigment
FURUNCULOSIS in Salmonidae