Lab 2 B. anthracis and Clostridium Flashcards
Bacillus Habitat morphology
Veg: diseased animals
Spore: environment
Rod, central spore, capsule
No flagella
Bacillus resistance
Veg: several days in carcass, 2 weeks on skin, 4 weeks in BM
Spore: soil - 50 years
Bacillus staining
Gr + Toluidine blue Metachromatic staining: Capsule: pink Bacteria: blue
Bacillus culture
Simple
Bacillus S/R colonies depend on…?
CO2
Bacillus capsule
Capsule (S): poly-D-glutamic acid
= Virulence factor
Inhibits phagocytosis
Requirements for spore formation:
> 15 degrees
Water, oxygen, cations
37 degrees: 16 hrs
18 degrees: 2-3 days
Bacillus biochemistry
Cat +, Oxi -
Bacillus antigens
Capsule
Polysaccharide hapten: heat stabile
Ascoli test
(B.cereus cross reaction)
Edema factor, Lethal factor
B. anthracis pathogenicity
Bo/Ov: fatal peracute/acute septicaemic anthrax
Su: subacute anthrax, pharyngeal edematous swelling
Eq: subacute anthrax with edema, septicaemia with colic/enteritis
Hu: skin, pulmonary and int form
Car/Av resistant
B. cereus
Bo mastitis
Hu food poisoning, eye infections
B. licheniformis
Bo/Ov sporadic abortion
Paenibacillus larvae
Honey bees: americal foulbrood (notifiable disease)
Saprophyte bacilli
B. subtilis
B. megaterium
B. cereus
B. licheniformis (bacitrin)
B. thuringiensis: insect pathogen, pest-control (moth)
Paenibacillus polymyxa
Geobacillus stearothermophilus (heat resistant)
Bacillus without flagella
B. anthracis
Bacillus with capsule prod in air
B. subtilis
B- megaterium