Past Exam Questions (Midterm II) Flashcards
What is regulating the urea cycle?
Carbamoyl Phosphate Synthetase I
How much energy is produced by the complete oxidation of 1 mol of glucose?
36 mol ATP
What creates NADPH?
- PPP
- FA synthesis
What is the start molecule of GNG?
Pyruvate
What are the start molecules of the citrate cycle?
Ac-CoA + OAC
Where is ketosis not found?
Liver
Which amino acid is essential in birds?
Arginine
Which amino acid produces GABA?
Glutamate (Glutamic acid)
Where is fat produced?
- Liver
- Mammary gland
- Adipose tissue
What glucose concentration is found in ruminants?
2-3 mmol/l
What is the start molecule for ketogenesis?
Ac-CoA
Which enzymes uses TPP as a cofactor?
- Pyruvate dehydrogenase
- Pyruvate dehydrogenase complex
- Transaldolase/Transketolase
How much energy is created by anaerobic glycolysis of 1 mol glucose?
2 mol ATP
Which enzymes catalyse substrate level phosphorylation? (ATP production)
- Succinyl-CoA Synthetase
- Pyruvate Kinase
Where does ketolysis occur?
- Mitochondria
- Mammary gland
- Brain
- Muscle
Which enzyme regulates glycogenesis?
Hexokinase
Which enzyme regulates glycogenolysis?
Glycogen phosphorylase
Which enzyme regulates glyconeogenesis?
Fructose 1,6 biphosphatase
Which enzymes regulate glycolysis?
- Hexokinase
- Phosphofructokinase 1
- Pyruvate kinase
Which enzyme regulates TCA?
Citrate synthase
Which enzyme regulates PPP?
Glucose-6-phosphate dehydrogenase
What is a common intermediate between gly and glucose?
Pyruvate
What are the primary bile acids?
- Cholic acid
- Chenodeoxycholic acid
What splits urea in ruminants?
Bacterial urease
Which amino acid is essential in equine?
Lys
Where does B-oh occur?
Mitochondria
How much energy does palmitic acid produce?
129 ATP
Which reactions is NADH2 produced in?
- GL
- TCA
- FA b-oh
Which reactions is biotin found in?
- FA synthesis - Ac-CoA carboxylase
- β-ox. of odd carbons - Propionyl CoA carboxylase
Which of the following will not require NADPH + H+?
- Synthesis of oleic acid
- Synthesis of palmitic acid
- The beta-oxidation of fatty acid
- Each of these requires NADPH+ H+
The beta-oxidation of fatty acid
Which of the following is not a dehydrogenase in the TCA cycle?
- Isocitrate dehydrogenase
- Alpha- ketoglutarate dehydrogenase
- Pyruvate dehydrogenase
- Succinate dehydrogenase
- Malate dehydrogenase
Pyruvate dehydrogenase
Alanine transaminase(ALT) transfers an amino group from alanine to…
Alpha-ketoglutarate
Which of the following statements about the reactions of glycolysis is true?
- In glycolysis glucose – 6 – phosphate is split into glyceraldehyde – 3- phosphate and dihydroxyacetone-phosphate
- In glycolysis glucose – 1,6 – biphosphate is split into glyceraldehyde – 3- phosphate and dihydroxyacetone-phosphate
- In glycolysis fructose – 6- phosphate is split into glyceraldehyde – 3- phosphate and dihydroxyacetone-phosphate
- In glycolysis glucose – 6 – phosphate is isomerized to fructose 1,6 – biphosphate
In glycolysis glucose – 1,6 – biphosphate is split into glyceraldehyde – 3- phosphate and dihydroxyacetone-phosphate
This enzymatic reaction takes place in the mitochondria
- 6 – phosphogluconate dehydrogenase
- Pyruvate kinase
- Succinate dehydrogenase
- Glucokinase
- All of these answers are true
All of these answers are true
Which contains B-D-glucose
- Starch
- Maltose
- Glycogen
- Cellulose
- Hyaluronic acid
Cellulose
From which molecule can oxaloacetate be produced?
- Malate
- Pyruvate
- Aspartate
- All of the answers are false 1-3
- All answers 1-3 are true
All answers 1-3 are true
Which pathway reaction takes place in the mitochondrion?
- Synthesis of palmitic acid
- Beta-oxidation of palmitic acid
- Glycolysis
- Glycogenesis
- All of the answers A-D is false
Beta-oxidation of palmitic acid
Which of the following statements about the generation of ATP in the electron transport chain is true?
- The generation of ATP from ADP coupled to electron transfer occurs by substrate-level phosphorylation as in glycolysis
- Electron transport generates a proton gradient across the outer mitochondrial membrane
- ATP synthase in valves are structured outside the inner mitochondrial membrane
- ATP synthase in valves are structures outside the outer mitochondrial membrane
ATP synthase in valves are structured outside the inner mitochondrial membrane
Which enzyme uses NADP+ as a cofactor?
- 6–P–gluconate dehydrogenase
- Lactate dehydrogenase
- Glyceraldehyde – 3- P – dehydrogenase
- Succinate dehydrogenase
- All of the answers 1-4 is false
6–P–gluconate dehydrogenase
Which of the following statements about the ketone bodies/acetoacetate and B- hydroxybutyrate is true?
- Muscles produce ketone bodies from fatty acids
- Adipose tissue can produce ketone bodies from its fat stores
- The liver produces acetoacetate and B-hydroxybyturate from fatty acids
- Acetoacetate is metabolized to acetate in peripheral tissue
The liver produces acetoacetate and B-hydroxybutyrate from fatty acids
Increased cAMP concentration increases the activity of which of the following enzymes?
- Phosphorylase kinase
- Glycogen phosphorylase
- Hormone-sensitive lipase
- All of these answers are false
- All of these answers are true
All of these answers are true
Alpha–ketoglutarate is the end product of the reaction catalyzed by the following enzyme?
- Pyruvate carboxylase
- Lactate dehydrogenase
- Pyruvate dehydrogenase
- Glutamate decarboxylase
- None of them
None of them
The start molecule of ketogenesis
- Propionyl-CoA
- Glycerol
- Glutamic acid (glutamate)
- Acetyl-CoA
Acetyl-CoA
How much energy is produced in the anaerobic glycolysis of 1 mol glucose?
- 2 mol ATP
- 3 mol ATP
- 36 mol ATP
- 129 mol ATP
- All of the answers A–D is false
2 mol ATP
Which of the following enzymes need thiamine pyrophosphate (TPP) as cofactor?
- Lactate dehydrogenase
- L–glutamate dehydrogenase
- Serine decarboxylase
- Pyruvate dehydrogenase
- Pyruvate carboxylase
Pyruvate dehydrogenase
Which of the following molecules contains the most carbon atoms?
- Tyrosine
- Tyramine
- Palmitic acid
- Histidine
Palmitic acid
The start molecule of ketogenesis
- Leucine
- Glutamic acid
- Propanyl–Coa
- All of them is false
- All of them is true
Leucine
Which pathway reaction takes place in mitochondria?
- Synthesis of palmitic acid
- Beta-oxidation of palmitic acid
- Glycolysis
- Synthesis of cholesterol
- None of them
Beta-oxidation of palmitic acid
How much energy is produced in the complete oxidation of 2 mol glucose?
- 72
- 3
- 36
- 129
- None of them
72
Characteristics of arachidonic acid?
- Omega 6 unsaturated fatty acids, four double bonds, 20 C
- Omega 3, unsaturated fatty acids, three double bonds, 18 C
- Omega 6 unsaturated fatty acids, two double bonds, 18 C
- Omega 3 unsaturated fatty acids, four double bonds, 20 C
- None of them
Omega 6 unsaturated fatty acids, four double bonds, 20 C
Which hasn’t got a high energy bond?
Glucose-6-Phosphate
The start molecule of GNG
Pyruvate
From which molecule can oxaloacetate be produced?
- Pyruvate
- Acetoacetate
- Leucine
- None of them
- All of them
Pyruvate
This molecule can be synthesized from HMG-CoA in a given pathway
- Acetate
- Acetone
- 3-Acyl-glycerol
- All of them are
- None of them
None of them
Which of the following is a common intermediate in the conversion of Gly to glucose?
- Phosphoenolpyruvate
- Fructose-1,6-bisphosphate
- Malate
- Oxaloacetate
- Pyruvate
Pyruvate
Which enzyme catalyzes reaction where substrates level phosphorylation happens?
- Succinyl CoA synthetase/Pyruvate kinase
- Succinate dehydrogenase
- Acetyl-CoA carboxylase
- Glutamine synthetase
- None of them
Succinyl CoA synthetase/Pyruvate kinase
Which establishment is not characteristic for the complex of the respiratory chain?
- Collector is FMN
- Hydrogen uptake from any NADH + H+
- Contains HEM iron
- Contains proton channel
- None of them
Contains HEM iron
Characteristic of linoleic acid?
- 6 unsaturated fatty acids, two double bonds, 20 C
- 3 unsaturated fatty acids, three double bonds, 18 C
- 6 unsaturated fatty acids, two double bonds, 18 C
- 3 unsaturated fatty acids, three double bonds, 20 C
6 unsaturated fatty acids, two double bonds, 18 C
In the urine of a healthy cat can be found?
- Urea, uric acid and ammonium ion
- Urea, Allantoin, ammonium ion
- Only urea
- Only uric acid
- None of them
Urea, Allantoin, ammonium ion
In the urine of a healthy dalmatian dog can be found?
- Urea, uric acid and ammonium ion
- Urea, Allantoin, ammonium ion
- Only urea
- Only uric acid
- None of them
Urea, uric acid and ammonium ion
The start molecule of gluconeogenesis
- Acetoacetate
- Leucine
- Glutathione
- Lactate
- All of them
Lactate
How many energy is produced in the beta-oxidation of 1 mol palmitoyl-CoA?
- 129 mol ATP
- 131 mol ATP
- 146 mol ATP
- 148 mol ATP
- None of them
131 mol ATP
This reaction happens in the cytoplasm
- ATP citrate lyase
- Fatty acyl-CoA dehydrogenase
- Succinate dehydrogenase
- HMG-CoA lyase
- None of them
ATP citrate lyase
Start molecule of oxaloacetate synthesis
Pyruvate
Allosteric enzyme
Reversible enzyme inhibition
Which amino acids can make pyruvate?
- Ala
- Ser
- Gly
- Cys
Which amino acid can make glutamic acid?
Alanine
Location of transaminases
Cytoplasm/Mitochondria
Give examples of VFAs
- Acetate
- Propionate
- Butyrate
Which compounds can be produced from HMG-CoA?
- Cholesterol
- KBS
Which enzyme is responsible for the transformation of pyruvate to oxaloacetate?
Pyruvate carboxylase
What are the uncoupling factors?
- Dinitrophenol
- Thermogenine
Which fatty acid has the longest chain?
DHA (22C)
Glucose level in birds
8-9mmol/l
Glucose level in dogs
4-5mmol/l
What is the first messenger in fat metabolism?
- Adrenaline
- Noradrenaline
- Glucagon
- ACTH
The activity of which enzyme is needed to produce a cofactor for the reaction catalysed by the glutathione reductase?
Glucose-6-phosphate dehydrogenase
Alpha-ketoglutarate is the product of the enzymatic reaction catalysed by the enzyme…
L-Glutamate dehydrogenase
Which enzyme is in use in the cytoplasm?
- Pyruvate dehydrogenase
- Pyruvate kinase
- ATP synthase
- Enoyl hydratase
- L-glutamate dehydrogenase
Pyruvate kinase
Which contains alpha-D-glucose?
- Starch
- Lactose
- Heparin
- Cellulose
- Chondroitin sulphate
Starch
In the urine of a healthy cat, what can be found?
- Bilirubin I
- Urea
- Myoglobin
- All answers are true
- All answers are false
Urea
Oxaloacetate is made from an intermediary product of the anaerobe glycolysis by which reaction?
Carboxylation
Which molecule can be produced in the intestine of a healthy dog?
- Bilirubin I (indirect)
- Bilirubin II (direct)
- Biliverdin
- Urobilinogen
- All answers are false
Urobillinogen
Which pathway/reaction takes place in the matrix of the mitochondrion?
- B-oxidation of palmitic acid
- Glycolysis
- Respiratory chain
- All answers are true
- All answers are false
B-oxidation of palmitic acid
Ketone bodies can be produced by which molecule?
- Glycerol
- Glutamate
- Propionyl-CoA
- Acetyl-CoA
- All answers are false
Acetyl-CoA
Propionyl-CoA carboxylase converts propionyl-CoA to which of the following molecules?
- Malonyl-CoA
- Methylmalonyl-CoA
- Succinyl-CoA
- All answers are false
- All answers are true
Methylmalonyl-CoA
Which of the following molecules is a ketone body?
- Acetoacetate
- Alpha-ketoglutarate
- Succinate
- Oxaloacetate
- Pyruvate
Acetoacetate
Substrate (starting molecule) of gluconeogenesis
- Propionate
- Acetoacetate
- Leucine
- Acetate
Propionate
How much energy is produced in the B-oxidation of 1 mol palmitoyl-CoA?
131 mol ATP
(-2 for charging fatty acid = 129 ATP)
Give the transporter of Fe2+ ions on the basolateral side of the enterocytes
Ferroportin
Which of the following molecules can be found in the urine of chicken under physiological conditions?
- Acetic acid
- Allantoin
- Ammonia
- Uric acid
- Urea
Uric acid
Which of the following enzymes has a role in the synthesis (B-reduction) of palmitic acid?
- Fatty-acyl-carnitine transferase I
- B-ketothiolase
- 1st reductase (B-ketoacyl-S ACP reductase)
- All answers are true
- All answers are false
1st reductase (B-ketoacyl-S ACP reductase)
Which of the following is an uncoupling factor?
- CO
- Thermogenin
- Myoglobin
- cAMP
Thermogenin
Which of the following pathways results in cellular NADPH + H+ production?
- Glycolysis
- Urea cycle
- TCA cycle
- PPP
- All answers are false
PPP
Which is produced in the citrate (TCA) cycle?
- NADH + H+
- FADH2
- GTP
- All answers are true
All answers are true
- NADH + H+
- FADH2
- GTP
Which enzyme of the citrate (TCA) cycle needs TPP as a cofactor?
Alpha-ketoglutarate dehydrogenase
Which molecule can be produced by the transamination of aspartate?
Oxaloacetate
Which of the following enzymes can be found in the mitochondrial matrix of the liver?
- Glyceraldehyde-3-phosphate dehydrogenase
- HMG-CoA reductase
- HMG-CoA lyase
- All answers are true
- All answers are false
HMG-CoA lyase
Which of the following molecules is produced by the decarboxylation of aspartate?
- B-alanine
- GABA
- Alpha-ketoglutarate
- Glutamine
- All answers are true
B-alanine
Which group of molecules does lecithin belong to?
- Sphingolipids
- Carotenoids
- Glycerol phosphatides
- Steroids
Glycerol phosphatides
Which of the following pathways doesn’t happen in the liver of the healthy horse?
- Conjugation of bilirubin with glucuronic acid
- Synthesis of primary bile acids
- Degradation of ketone bodies (ketolysis)
- B-oxidation of fatty acids
Degradation of ketone bodies (ketolysis)
How many mol ATP will be produced from one mol FADH2 in oxidative phosphorylation?
2 mol ATP
Citrate cycle begins with the condensation of
- Propionyl-CoA and oxaloacetate
- Acetyl-CoA and oxaloacetate
- 2 x acetyl-CoA
- All answers are false
Acetyl-CoA and oxaloacetate