Parasitology Exam 2 Flashcards
- eggs can be mamalated (bumpy) or smooth when fertilized
- eggs are barrel-shaped when unfertilized
- requires both male and female worm present to fertilize
Ascaris lumbricoides
Ascaris lumbricoides: Diagnostic and Infective
Diagnostic: eggs
Infective: eggs
- eggs have “flat football” appearance (one flat side)
- eggs collected from the peri-anal area with a sticky-sided spatula
Enterobius vermicularis (Pinworm)
Enterobius vermicularis: Diagnostic and Infective
Diagnostic: eggs
Infective: eggs
- eggs have distinctive “polar plugs” and are barrel-shaped
- light amber color on bile stain
Trichuris trichuria (Whip worm)
Trichuris trichuria: Diagnostic and Infective
Diagnostic: eggs
Infective: eggs
- eggs have granular cluster of larvae with visible space between them and the cell wall
- two organisms have the same egg morphology
Ancylostoma duodenale (Old World Hookworm)
Necator americanus (New World Hookworm)
Hookworm: Diagnostic and Infective
Diagnostic: eggs
Infective: filariform larva penetrates skin
“Worm burden”
a measure of how many hookworms are in the gut, can relate to the severity of iron deficiency anemia that may develop from chronic bleeding
Differences in adult hookworm morphology
- Ancylostoma duodenale has 2 pairs of teeth for attachment
- Necator americanus has two cutting plates for attachment
Differences between strongyloides and hookworm rhabditiform larvae
- Strongyloides has short buccal cavity
- Hookworm has long buccal cavity
Which nematode can cause autoinfection?
Strongyloides stercoralis
Strongyloides stercoralis: Diagnostic and Infective
Diagnostic: rhabditiform larvae
Infective: filariform larva penetrates skin
Usually obtained from infected animal tissue, especially raw pork. “Wood knots” appearance in tissue
Trichinella spiralis
Trichinella spiralis: Diagnostic and Infective
Diagnostic: Larvae in tissue
Infective: encysted larvae in undercooked meat
Adult worm releases larvae from a blister on the leg or foot. Eliminated by slowly winding on a stick.
Dracunulus medinensis
Dracunulus medinensis: Diagnostic and Infective
Diagnostic: Blister bursting
Infective: ingesting copepods in water
- Causes elephantiasis of the lower extremities
- Microfilariae in the blood have a sheath, but no nuclei in the tip of the tail
Wucheria bancrofti
Wucheria bancrofti: Diagnostic and Infective
Diagnostic: microfilaria in blood
Infective: skin penetration of microfilariae by insect vector (Anopheles)
- Causes elephantiasis of the lower extremities
- Microfilariae in the blood have a sheath and 2 terminal nuclei in the tail
Brugia malayi
Brugia malayi: Diagnostic and Infective
Diagnostic: microfilaria in blood
Infective: skin penetration of microfilariae by insect vector (Anopheles)
- Microfilariae in the blood have a sheath and nuclei are continuous to tip of the tail
Loa Loa (eye worm)
Loa Loa: Diagnostic and Infective
Diagnostic: Calibar swelling (nodules), seeing worm migrate
Infective: Larvae are spread by insect vector (sandfly, deerfly, chrysops)
- Causes “River blindness”
- Subcutaneous nodules known as “ochocercoma” where the adult lives
- Microfilariae in the blood have NO sheath, nuclei do NOT extend to tip of tail
Onchocerca volvulus
Onchocerca volvulus: Diagnostic and Infective
Diagnostic: biopsy skin snips of nodule
Infective: larvae spread by bite of gnat
- Cause largely asymptomatic filarial roundworm infections
- Inhabit body cavities
- Spread by insect vector
- Mansonella ozzardi (unsheathed, no nuclei to tip of tail)
- Mansonella perstands (unsheathed, nuclei extend to tip of tail)
- Eggs have a “ruffled edge” appearance
- Eggs are almost circular
- Eggs are thick-walled, have 3 pairs of hooklets for attachment
Taenia (saginata and solium, egg can’t be distinguished)
Taenia: Diagnostic and Infective
Diagnostic: Egg or proglottid in stool
Infective: Encysted larva in undercooked meat
Taenia saginata morphology
- Proglottids are longer than wide
- Proglottids have15-20 uterine branches/side
- Adult scolex has 4 suckers, no hooks (unarmed)
Taenia solium morphology
- Proglottids are longer than wide
- Proglottids have <13 uterine branches/side
- Adult scolex has 4 suckers, with hooks (armed)
Which cestode can encyst in other parts of the body (cysticercosis)?
Taenia solium
- Egg is 30-50 um
- Egg has hooked oncosphere
- Egg has polar filaments
Hymenolepis nana (dwarf tapeworm)
- Egg is 60-80 um
- Egg has hooked oncosphere
- Egg has NO polar filaments
Hymenolepis diminuta (rat tapeworm)
- Tapeworm commonly infects dogs and cats
- Forms characteristic egg packets (8-15 eggs)
- Egg packets measure 25-40 um
Dipylidium caninum
- adult has spoon-shaped scolex
- Proglottids are wider than long, central uterine pore
- Egg has lidded operculum, terminal knob on avoperculum
Diphyllobothrium latum (fish tapeworm)
Diphyllobothrium latum: Diagnostic and Infective
Diagnostic: egg
Infective: cysts in undercooked fish
What cestode can lead to megaloblastic anemia due to B12 deficiency?
Diphyllobothrium latum
- normal life cycle involves dogs and sheep
- forms hydatid cysts filled with hydatid sand in tissues of humans
Echinococcus granulosus
What happens if a hydatid cyst bursts during surgical removal?
Dissemination of the daughter cells within, possible anaphylaxis if cyst leakage has previously stimulated an IgE response
Echinococcus granulosus: Diagnostic and Infective
Diagnostic: Cyst
Infective: Egg
- Self-resolving itchy skin after contact with water
Schistasome Dermatitis, aka “swimmer’s itch”. Caused by avian schistosomes that can’t penetrate deeper than the skin
- Egg shows a terminal spine
- Leading cause of bladder cancer in Egypt
- Lives in blood vessels supplying the bladder
Schistosoma haemotobium (bladder fluke)
Schistosoma haemotobium: Diagnostic and Infective
Diagnostic: Eggs found in URINE
Infective: Cercariae penetrates skin
- Intestinal schistosome
- Egg has large lateral spine
- Egg is large (160x80 um)
Schistosoma mansoni
Schistosoma mansoni: Diagnostic and Infective
Diagnostic: egg in stool
Infective: Cercariae penetrates skin
- Intestinal schistosome
- Egg has small lateral spine (can be hard to see)
- Egg is small (80x90 um)
Schistosoma japonicum (Oriental Blood Fluke)
Schistosoma japonicum: Diagnostic and Infective
Diagnostic: egg in stool
Infective: Cercariae penetrates skin
- Large operculated egg (55x85 um)
- Shouldered operculum
Paragonimus westermanni (Oriental Lung Fluke)
Paragonimus westermanni: Diagnostic and Infective
Diagnostic: egg seen in SPUTUM
Infective: eating cysts in undercooked crayfish
- Very large operculated egg (150x90 um)
- Densely granulated egg, amber color
Fasciola hepatica (Sheep Liver Fluke)
or
Fasciolopsis buski (Intestinal Fluke)
Fascioloa hepatica: Diagnostic and Infective
Diagnostic: egg in stool
Infective: Eating cysts in contaminated watercress
Fasciolopsis buski: Diagnostic and Infective
Diagnostic: egg in stool
Infective: Eating cysts in contaminated water chestnuts
- tiny egg (30x16 um) with shouldered operculum
- egg has terminal knob (not always visible)
- smallest fluke
Clonorchis sinensis (Chinese Liver Fluke)