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MLS > Anaerobes Exam > Flashcards

Anaerobes Exam Flashcards

1
Q
  • “Boxcar” GPR
  • RARE terminal or subterminal spores
  • Catalase =
  • Double Zone of Beta Hemolysis
  • Lecithinase +
  • Reverse CAMP +
  • Non-motile
A

Clostridium perfringens

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2
Q
  • Oval GPR, subterminal spore
  • Yellow, ground-glass on CCFA
  • Cytotoxins A and B
  • Catalase =
  • Motile
A

Clostridioides difficile

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3
Q
  • Long GPR, terminal or subterminal spore
  • Causes botulism
  • Hard to culture
  • Catalase =
  • Motile
A

Clostridium botulinum

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4
Q
  • GPR with subterminal spores
  • Beta-hemolytic, medusa head rhizoid margin
  • Catalase =
  • Motile
  • Lecithinase =
  • Lipase =
  • Indole =
  • Bile Esculin +
A

Clostridium septicum

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5
Q
  • GPR with terminal spore
  • “Swarming” colony
  • Produces tetanospasmin neurotoxin
  • Catalase =
  • Motile
A

Clostridium tetani

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6
Q
  • GPR with subterminal or free spores
  • Mottled internal colony structure
  • 7 exotoxins
  • Catalase =
  • Motile
  • Lecithinase +
  • Lipase =
  • Indole +
  • Urea +
A

Clostridium sordellii

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7
Q
  • Filamentous GPR, no spores
  • Young colonies “spider like”
  • Old colonies “molar tooth”
  • Sulfur granules in abscess drainage
  • Catalase =
  • Partial acid fast =
  • Nitrate +
A

Actinomyces spp.

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8
Q
  • “Chinese letter” GPR
  • can resemble diphthroids or be club-shaped
  • Normal flora
  • Catalase +
  • Indole +
A

Cutibacterium Acnes/ Propionbacterium spp.

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9
Q
  • Uniform to pleomorphic GPR, pairs or clumps
  • Small, circular, translucent colonies
  • Catalase =
  • Indole =
  • Nitrate +
A

Eggnerthella lenta

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10
Q
  • Uniform to pleomorphic GPR, pairs or clumps
  • Small, circular, translucent colonies
  • Catalase =
  • Indole =
  • Nitrate =
A

Eubacterium spp.

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11
Q
  • GPCB with bent, pointed, or fork-shaped ends
  • Catalase =
  • Indole =
  • Nitrate =
  • Bile Esculin +
A

Bifidobacterium spp.

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12
Q
  • Pleomorphic GPR
  • Normal flora of mucus membranes
A

Lactobacillus spp.

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13
Q
  • GNR, faint staining
  • Kanamycin - R
  • Vancomycin - R
  • Colistin - R
  • Bile - R
  • Hydrolyzes esculin, turning BBE agar black/brown
A

Bacteroides fragilis

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14
Q
  • Microaerophile
  • Thin GNR with round ends
  • Kanamycin - S
  • Vancomycin - R
  • Colistin - S
  • Bile - S
  • Catalase =
  • Indole =
  • Nitrate =
A

Campylobacter ureolyticus

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15
Q
  • GNCB or pleomorphic GNR
  • Colony may have pigment (fluoresce red if so)
  • Fluoresce pink, orange, or chartreuse otherwise
  • Grows black/brown on KVLB
  • Kanamycin - R
  • Vancomycin - R
  • Bile - S
A

Provotella spp.

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16
Q
  • GNR
  • Fastidious - requires Hemin and VitK
  • Black/brown colonies
  • Fluoresce red
  • Indole +
  • Kanamycin - R
  • Vancomycin - S
  • Colistin - R
  • Bile - S
A

Porphyromonoas spp.

17
Q
  • Thin, fusiform GNR
  • Makes butyric acid (smelly)
  • “Bread crumb” colonies
  • Fluoresce chartreuse
  • Catalase =
  • Nitrate =
  • Indole +
  • Lipase =
  • Kanamycin - S
  • Vancomycin - R
  • Colistin - S
  • Bile - S
A

Fusobacterium nucleatum

18
Q
  • Globular, swollen, bizarre shaped GNR
  • Makes butyric acid (smelly)
  • Lemierre’s Disease (Jugular vein thrombosis)
  • Fluoresce chartreuse
  • Catalase =
  • Nitrate =
  • Indole +
  • Lipase +
  • Kanamycin - S
  • Vancomycin - R
  • Colistin - S
  • Bile - S
A

Fusobacterium necrophorum

19
Q
  • GPC
  • SPS - R
A

Peptoniphilus spp.

20
Q
  • GPC
  • SPS - S
A

Peptostreptococcus spp.

21
Q
  • GPC
  • Black colonies
A

Peptococcus niger

22
Q
  • GNC
  • May fluoresce red
  • Nitrate =
  • Kanamycin - S
  • Vancomycin - R
  • Colistin - S
  • Bile - S
A

Veillonella spp.

MLS (18 decks)

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