Parasitology Flashcards
Deals with organisms that take up their abodes, on or within other living organisms for procuring food
Parasitology
Concerned with the phenomenon of dependence of one living organism to another
Parasitology
Causes Infection
Endoparasite
Causes infestation
Ectoparasite
Found in an organ which is not its usual habitat
Erratic parasite
Abberant parasite, wandering parasite
Erratic parasite
Examples of ectoparasites
- Sarcoptes scabiei “itch mite”
- Pediculus humanus capitis and corporis “Dog and cat hookworm”
- Pthirus pubis “pubic lice”
One that remains on or in the body of the host for its entire life
Permanent parasite
One that lives on the host only for a short period of time (mosquito and bedbug)
Temporary parasite
Also known as Intermittent parasite
Temporary parasite
“Coprozoic”
Spurious parasite
Free-living organism that passes through the digestive tract without infecting the host
Spurious parasite
Parasite that causes injury to the host
Pathogenic parasite
Parasite that does not cause injury to the host
Non-pathogenic or commensal
Requires a host to live
Obligate
Has a free-living state; can also live inside the host
Facultative
Thrives in a host that is not its usual host
Accidental/Incidental
Requires only 1 host to complete life cycle
Monoxenous
Requires more than 1 host to complete life cycle
Heteroxenous
Harbors the adult and or the sexual stage of the parasite
Definitive host
Harbors the larva and or the asexual stage of the parasite
Intermediate
Harbors the parasite that does not undergo any development, remains alive, and is able to infect another susceptible host
Paratenic or Phoretic or Transport host
Living together of two unlike organisms.
May also involve protection, nourishment, and shelter to one or both organisms
Symbiosis
Eating at the same table
Commensalism
Both are benefitting
Mutualism
One benefitting, other is harmed
Parasitism
Required in the development of the parasite
Biological vector
For transport only
Mechanical or Phoretic or Transport or Harborage vector
thread-like, long whip-like structure
Flagella
false-feet
Pseudopodia
flexible sheet of material that joins the flagella to the surface of the cell
Undulating membrane
consist of polar rings, subpellicular tubules, conoid, rhoptries and micronemes;
for penetration and invasion of target cell
apical complex
Only pathogenic intestinal amoeba
Entamoeba histolytica
PC: fine, uniform, beaded appearance
K: central, small
“Bull’s eye appearance”
Entamoeba histolytica/dispar
Appearance of the cytoplasm of E. histolytica
Ground glass (clean-looking)
small race E. histolytica
E. hartmanni
Largest amoeba
E. coli
Cytoplasm of E. coli
Coarsely granular, vacuolated (dirty-looking)
“Smallest intestinal protozoan”
“Dwarf intestinal slug”
Endolimax nana
blot-like karyosome
Endolimax nana
Karyosome is large, central surrounded by refractile achromatic granules
Iodamoeba butschlii
chromatoidal bodies of E. histolytica/dispar
Blunt with rounded ends (cigar-shaped, sausage -shaped, rod-like)
Chromatoidal bodies of E. hartmanii
Elongated bar with blunt rounded ends (bar-like, rice grain)
Chromatoidal bars of Entamoeba coli
with pointed ends (splinter-like, broomstick, jagged ends)
N: Cross-eyed cyst, “button hole”
Endolimax nana
Nucleus is basket-shaped, “basket of flowers”
Iodamoeba butschlii
Swine amoeba, cyst is uninucleated, in pigs and monkeys
Entamoeba polecki
Pathogenic amoeba of humans, Capable of erythrophagocytosis
Entamoeba histolytica
infective stage of E. histolytica
Cyst
Diagnostic stage of E. histolytica
Formed stool: cyst
Water stool: trophozoite
Extraintestinal: trophozoite
Mode of transmission of E. histolytica
Fecal-oral, sexual
Ulcer made by E. histolytica
Flask-shaped
Hepatic amoebiasis (reddish brown or ___)
anchovy sauce abscess
Gold standard in the diagnosis of E. histolytica
Concentration technique
Culture media used in the diagnosis of E. histolytica
Boeck/ Drbohlav’s biphasic medium
Locke’s egg medium
Shaffer Ryden Frye medium
Balamuth’s medium
Robinson and Inokii
Stains used in the diagnosis of E. histolytica
Lugol’s iodine (cyst)
Quensel’s methylene blue (trophozoite)
Sucker-like structures for phagocytosis in culture forms of Naegleria fowleri
Amoebastomes
Infective and diagnostic stage of N. fowleri
Trophozoite
POE and MOT is the nasal mucous membrane, through swimming in brackish or fresh water (rarely in swimming pools and inhalation of cyst from the dust)
Naegleria fowleri
Disease caused by N. fowleri
Primary amoebic meningoencephalitis (PAM)
Stain used in the diagnosis of N. fowleri
Acridine orange
culture media used in the diagnosis of N. fowleri
Bacteria seeded agar (Gram-negative bacilli) –> trailing effect
CBC results of px with N. fowleri
Increased WBC - neutrophilia
CSF analysis (N. fowleri)
Increased PMNs, proteins
Decreased glucose
growth of this parasite is inhibited by 0.2% NaCl and Kcl
N. fowleri
Naegleria spp that is still viable after lyophilization for 6 months
Naegleria gruberi
Acanthamoeba spp has spinelike pseudopods called
acanthopodia
Infective and diagnostic stage of Acanthamoeba spp
Trophozoite
POE and MOT of Acanthamoeba spp
Eyes (infecting contact lenses)
Motility of Acanthamoeba spp
Sluggish, polydirectional motility
disease caused by Acanthamoeba spp
Granulomatous Amebic Encephalitis (GAE)
diseased caused by Acanthamoeba spp in immunocompetent hosts
Corneal ulcer/keratitis
stain used in corneal scrapings from Acanthamoeba infected areas
Calcafluor white stain
culture used in the diagnosis of Acanthamoeba spp
PYGC
Bacteria seeded agar (E. coli)
Technique of choice for the diagnosis of Acanthamoeba spp
Indirect Immunofluorescent Antibody
formerly classified as yeast; now a protozoan
Blastocystis hominis
form of B. hominis that causes diarrhea
Vacuolated form
Culture media used in the diagnosis of B. hoinis
Boeck/Drbohlav’s
Nelson and Jones
Optimal growth of B. hominis using Nelson and Jones culture medium
37C in the presence of bacteria
motility of G. lamblia
Falling leaf
“Old man with eyeglasses”
“Monkey face”
G. lamblia
Has VSP (Variant Specific Surface Proteins)
resistant to intestinal proteases
G. lamblia
Cytoplasm of G. lamblia is often retrated from cyst wall (especially seen in ____)
“Halo effect”
Formalinized specimen
Gay bowel syndrome
Leningrad’s curse (traveler’s diarrhea)
Backpacker’s diarrhea
Beaver fever
Giardia lamblia
Diagnosis of G. lamblia
Enterotest (String test)
Transmission of G. lamblia
Fecal-oral, sexual
Most sensitive and most specific diagnostic test for G. lamblia
Direct Fluorescent Antibody
virulence factor of G. lamblia
Sucking disc
leads to malabsorption diarrhea –> steatorrhea
G. lamblia
Prominent cytosome
Shepherd’s crook
Spiral groove
Rotary motility
Chilomastix mesnili
Cyst: Lemon-shaped with anterior, hyaline knob (nipple-like)
C. mesnili
amoeboid, “stellate” appearance
Dientamoeba fragilis
explosive disintegration in water
seen in D. fragilis
Hakansson phenomenon
Granules of D. fragilis demonstrates
Brownian movement
Concomitant with pinworm infection
D. fragilis
Diagnostic stage of D. fragilis
Binucleate trophozoite
undulating membrane extends entire length of the body
Pentatrichomonas hominis
Characteristic wobbly movement
Pentatrichomonas hominis
undulating membrane extends 1/2 length of the body
Rapid, jerky motility
Siderophil granules
Trichomonas vaginalis
Cause of strawberry cervix, pingpong infection
T. vaginalis
Undulating membrane extends 2/3 length of the body
Trichomonas tenax
Developmental stages of Blood flagellates
Amastigote
Promastigote
Epimastigote
Trypomastigote
Source or habitat of L. tropica, L. major, and L. mexicana
Cutaneous
Tissue juice
Vector of Leishmania spp
Phlebotomus
Disease caused by L. tropica complex
Cutaneous leishmaniasis
Source of L. braziliensis
Mucocutaneous
Source of L. donovani complex
Visceral blood
DS stage of Leishmania spp
Amastigote
Infective stage of Leishmania spp
Promastigote
Culture used in the diagnosis of Leishmania spp
Novy-Macneal-Nicolle medium
Leishmanin skin test
Montenegro skin test
Aldehyde test of Napier (formol gel test)
Leishmania spp
Result of Aldehyde test of Napier for Visceral Leishmaniasis
(+: jellification), negative for others
Antimony test of Chopra
Leishmania spp
Result of Antimony test of Chopra in CL
(+: flocculence)
neg for others
Vector for Trypanosoma spp
TseTse fly
Vector of Leishmania spp
Phlebotomus (sandflies)
Game-attacking
Glossina spp
Tsetse flies
T. brucei rhodesiense
Acute African or East African Trypanosomiasis
T. brucei rhodesiense
Other name of Sleeping sickness
Nagana’s Disease
RIverine Glossina spp
Tsetse flies
T. brucei gambiense
Chronic African or West African Trypnosomiasis
T. brucei gambiense
Causes “Winterbottom’s sign”
Trypanosoma spp
Causes “Kerandel’s Sign”
CNS involvement
Trypanosoma spp
DS of Trypanosoma spp
Trypomastigote
IS of Trypanosoma spp
Metacyclic Trypopmastigote
Anterior Station Development
Trypanosoma spp
C-shaped Trypomastigote
T. cruzi
Reduvid bug
Cone-nosed bugs
Kissing bugs
Assasin bug
Triatoma infestans
Pastrongylus megistus
Rhodnius prolixus
T. cruzi
vector of T. cruzi seen in the Philippines
Triatoma rubrofasciata
South American Trypanosomiasis
Chagas disease
T. cruzi
Causes chronic disease: affects heart; may also cause achalasia and megacolon
T. cruzi
Manifestation of infection cause by T. cruzi
Chagoma
Romana’s sign: swelling of the eyelids
T. cruzi
DS of T. cruzi
Trypomastigote (blood)
Amastigote (tissue)
IS of T. cruzi
Metacyclic Trypomastigote
Culture used in the diagnosis of T. cruzi
Chang’s medium
Novy-Macneal-Nicolle medium
Xenodiagnosis of T. cruzi
Triatomid Bugs
causes Posterior station development: stercorarian
T. cruzi
Largest protozoan parasite of humans
Balantidium coli
Reservoir of Balantidium coli
pigs
part of B. coli for locomotory, rotary motiion
Cilia
Part of B. coli for osmoregulation
Contractile vacuoles
Food vacuole of B. coli
Cystostome
Motility of B. coli
Rolling ball or Thrown Ball (rotary motility)
Disease caused by B. coli
Balantidiasis
Ulcer formed by B. coi
Wide neck and rounded base
does B. coli produces extraintestinal infection
NO
Diagnosis of B. coli
Microscopy or biopsy
actively dividing form by mutiple fission
Schizont
daughter cell from schizont
Merozoite
fusion of macrogamete and microgamete
Syngamy
product of syngamy
zygote
Intermediate host of Plasmodium spp
Man
vector of Plasmodium spp
Female anopheles mosquito
malignant tertian malaria
Blackwater fever
P. falciparum