Oxidase positive/ Misc GNR Flashcards
Vibrio cholerae infections
serious GI infection
associated with pandemics
rice-water stools
contaiminated water or sea food
Vibrio cholerae toxic strains
O1: Classic and El Tor (produce exotoxin that acts on GI cell lining)
O139
Vibrio parahaemolyticus infections by
undercooked/raw seafood
Japan “summer diarrhea”
Vibrio key tests
Catalase + oxidase + GF motile indole + nitrate + facultative anaerobes gelatin + KIA: AK/A (A/A vulnificus) LAO: +/=/+ (vulnificus +/=/v) TSI: A/A (cholera); AK/A (parahaemolyticus); AK/A OR A/A (vulnificus) S to vibriostatic agent 0/129 @10 ug & 150ug; (parahaemolyticus is R to 10ug)
Vibrio vulnificus infection
N. America/ Hawaii
seafood contam.
wounds in water
(predisposition for infections: chronic liver disease)
Thiosulfate Citrate Bile Salts Sucrose Selective Media (TCBS)
Selective & differential forVibrio
yellow= sucrose fermentation (cholera +)
blue/green= NSF (vulnificus & parahaemolyticus)
Alkaline peptone water (APW)
enrichment media for Vibrio
high pH inhibits contaiminants (9.0)
Vibrio is halophilic which means
growth in elevated salt concentration
Ferment inositol test
Vibrio =
Plesiomonas +
Which Vibrio can grow in a broth with no NaCl?
V. cholera
Which Vibrio will not grow in 6.5% salt?
V. cholera
Vibrio vulnificus key tests
ferments salicin, lactose TSI: AK/A grows in both 1% & 6.5% salt ONPG + vp= insolitol = sucrose = LAO: +/=/+ motile indole + S vibriostatic agent 0/129
Vibrio cholera key tests
ONPG + NLF VP+ salicin = sucrose + growth 0-3% salt motile indole + S to vibriostatic agent 0/129 \ LAO +/=/+
Vibrio parahaemolyticus key tests
ONPG = NLF VP= SALICIN/SUCROSE= grows in 3-8% salt LAO +/=/+ MOTILE INDOLE + S to vibriostatic agent 0/129
Vibrio alginolyticus key tests
ONPG = NLF VP+ Salicin = sucrose + insolitol = grows in 3-10% salt motile, indole +, S vibriostatic agent 0/129 LAO; +/=/+