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Micro CCMLS > Oxidase positive/ Misc GNR > Flashcards

Oxidase positive/ Misc GNR Flashcards

1
Q

Vibrio cholerae infections

A

serious GI infection
associated with pandemics
rice-water stools
contaiminated water or sea food

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2
Q

Vibrio cholerae toxic strains

A

O1: Classic and El Tor (produce exotoxin that acts on GI cell lining)
O139

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3
Q

Vibrio parahaemolyticus infections by

A

undercooked/raw seafood

Japan “summer diarrhea”

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4
Q

Vibrio key tests

A 
Catalase +
oxidase +
GF
motile
indole +
nitrate +
facultative anaerobes
gelatin +
KIA: AK/A (A/A vulnificus)
LAO: +/=/+ (vulnificus +/=/v)
TSI: A/A (cholera); AK/A (parahaemolyticus); AK/A OR A/A (vulnificus)
S to vibriostatic agent 0/129 @10 ug & 150ug; (parahaemolyticus is R to 10ug)
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5
Q

Vibrio vulnificus infection

A

N. America/ Hawaii
seafood contam.
wounds in water
(predisposition for infections: chronic liver disease)

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6
Q

Thiosulfate Citrate Bile Salts Sucrose Selective Media (TCBS)

A

Selective & differential forVibrio
yellow= sucrose fermentation (cholera +)
blue/green= NSF (vulnificus & parahaemolyticus)

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7
Q

Alkaline peptone water (APW)

A

enrichment media for Vibrio

high pH inhibits contaiminants (9.0)

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8
Q

Vibrio is halophilic which means

A

growth in elevated salt concentration

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9
Q

Ferment inositol test

A

Vibrio =

Plesiomonas +

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10
Q

Which Vibrio can grow in a broth with no NaCl?

A

V. cholera

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11
Q

Which Vibrio will not grow in 6.5% salt?

A

V. cholera

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12
Q

Vibrio vulnificus key tests

A 
ferments salicin, lactose
TSI: AK/A
grows in both 1% & 6.5% salt
ONPG +
vp=
insolitol =
sucrose =
LAO: +/=/+
motile
indole +
S vibriostatic agent 0/129
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13
Q

Vibrio cholera key tests

A 
ONPG +
NLF
VP+
salicin =
sucrose +
growth 0-3% salt
motile
indole +
S to vibriostatic agent 0/129 \
LAO +/=/+
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14
Q

Vibrio parahaemolyticus key tests

A 
ONPG =
NLF
VP=
SALICIN/SUCROSE=
grows in 3-8% salt
LAO +/=/+
MOTILE
INDOLE +
S to vibriostatic agent 0/129
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15
Q

Vibrio alginolyticus key tests

A 
ONPG =
NLF
VP+
Salicin =
sucrose +
insolitol =
grows in 3-10% salt
motile, indole +, S vibriostatic agent 0/129
LAO; +/=/+
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16
Q

Aeromonas hydrophia infection

A

aquatic
assoc. w/ meats, produce, seafood
intestinal diarrhea and gastroenteritis
septicemia and wound infec (trauma involving water)

17
Q

Aeromonas hydrophlia looks like what other bacteria?

A

Escherichia coli

Micro CCMLS (12 decks)

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