Media/ Tests Flashcards
Trypticase soy agar
supportive media (non-fastidious)
BAP
enrichment (fastidious)/ selective/ differential
contains: 5% sheep blood agar trypticase soy base
ID aerobic GPC
Hemolysis: strep only report Enterococcus/group D gamma, GAS/GBS/group C,F,G beta, viridian/pneumonia alpha
BCYE
buffered charcoal yeast extract
enrichment media
contains: L-cysteine (enrichment)
Legionella pneumophila
CVA CAMPY
Campylobacter-blood agar
enrichment/ selective
contains: AB (selective), Brucella agar (enrichment) (sheep blood, yeast extract, casein)
Campylobacter; inhibits GI flora
increase temp 42*C; micro-aerophilic environment
Chocolate Agar
enrichment
contains: hemin (x factor) and NAD (V factor), vit B12, Fe & Mg, Cysteine
Neisseria and Haemophilus
PEA
phenyl-ethyl alcohol agar
selective
contains: sheep BA, phenylethyl alcohol
isolates GPC from mixtures; inhibits GNR
MAC
MacConkey agar selective/differential contains: lactose (differential), bile salts (selective), crystal violet, neutral red indicator isolates GNR ferments lactose- pink non fermenter- colorless
S-MAC
Sorbitol MacConkey
differential
contains: lactose, bile salts, crystal violet, neutral red indicator
ID E. coli O157:H7 sorbitol negative (clear)
SXT
sulfamethoxazole/trimethoprim
selective/differential
contains: SXT (antimicrobial against normal throat flora alpha Strep)
isolate GAS
HIM
Hemophilus isolation media
enrichment/differential
contains: X (Hemin) and V (NAD) factors, horse blood,
ID Haemophilus
CNA
columbia colistin-naldixic acid
selective
contains: colistin, naldixic acid
selects for GP
Hektoen agar
differential/selective
contains: bile salts (inhibit GP), lactose, sucrose, salicin (if use turn yellow), Na thiosulfate (H2S prod.= black color)
ID Salmonella spp & Shigella spp.= colorless
Salmonella black
XLD
xylose-lysine-desoxycholate agar
selective/differential
contains: bile salts (inhibit GP), latose, sucrose, xylose (yellow), Na thiosulfate (black)
ID Salmonella spp, & Shigella spp. = colorless
Salmonella black
Thayer Martin agar
enrichment/selective
contains: vancomycin (inhibit GPC), colistin (inhibit GNR), trimethoprim (stop Proteus swimming), nystatin (inhibits fungus)
Isolate Neisseria gonorrhoeae
Martin Lewis agar
enrichment/selective
contains: anisomycin (antifungal), vancomycin (more than Thayer Martin agar)
Neisseria isolates
V-agar
vaginalis agar
enrichment
contains: human blood, peptones, yeast extract
isolates Gardnerella vaginalis
MSA
mannitol salt agar
contains: peptones, beef extracts, 7.5% NaCl, mannitol, phenol red
ID Staphylococcus aureus (ferments mannitol= yellow)
Thioglycolate Broth
holding media (wounds & fluid cultures) supports aerobic (top)/anaerobic(bottom) growth contains: casein, soybean peptones, NaCl, Na thioglycollate
GN and Selenite Broth
support growth of Salmonella spp. & Shigella spp.
stool cultures subbed in this
good for small #s
Coagulase tube test
Staph rxts w/ coagulase-reacting factor (thrombin like thermostable molecule) to form complex that converts fibrinogen to fibrin
Rapid agglutination S. aureus
Use latex that detects clumping factor (w/ fibrinogen) and protein A (w/ IgG)
Coagulase slide test
Clumping factor causes agglutination in human/rabbit/pig plasma by converting fibrinogen to fibrin
sodium hippurate test
tests if bacteria can hydrolyze sodium hippurate to hippuric acid, glycine, and benzoic acid.
glycine deaminated by ninhydrin (oxidizer)
end prod. of oxidation forms purple dye
CAMP test
ID Streptococcus agalactiae & Listeria monocytogenes
prod. proteins that interact with beta hemolysin in S. aureus to produce enhanced hemolysis
streak S. aureus in middle, test streak perpendicular to Staph but not touching
S. agalactia- arrow shaped zone of hemolysis
L. monocytogenes- rectangular shaped zone of hemolysis