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Micro CCMLS > Mycobacterium > Flashcards

Mycobacterium Flashcards

1
Q

Photochromogen

A

Produces pigment in light

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2
Q

Scotochromogen

A

Produces pigment in light and dark

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3
Q

Non photochromogen

A

Produces no pigment

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4
Q

Photochromogenic mycobacterium

A

M. Kansasii
M. marinum

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5
Q

Scotochromogenic mycobacterium

A

M. Scrofulaceum
M. Gordonae

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6
Q

Non photochromogenic mycobacterium

A

M. Avium
M. Ulcerans
M. Fortuitum
M. Avium complex
M. Tuberculosis

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7
Q

Rapid growing mycobacterium

A

M. Foruitum
M. Chelonei

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8
Q

Mycobacterium tuberculosis

A

Niacin +
Nitrate +
Rough buff serpentine cording on culture

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9
Q

Mycobacterium avium complex

A

Environmental
Pulmonary/ disseminated disease in immuno.
Non pigmented on LJ
ID by nucleic acid probes
Tellurite+

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10
Q

Mycobacterium leprae

A

Hansen disease
Leprosy
Treatable Progressive disease of skin/ mucous mem/ nerves
Grows best in armadillo footpads

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11
Q

Lowenstein Jensen

A

LJ
60% egg, malachite green
Heat to 85*C until protein coagulates

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12
Q

Cauliflower colonies on LJ

A

M. Tuberculosis

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13
Q

Tween 80

A

Oleic acid
Aids in dispersing mycobacterium colonies in liquid media

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14
Q

Decontamination of mycobacterium; NALC

A

N-acetyl-L-cysteine (mucolytic)
2% NaOH (decontaminates)
Time dependant

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15
Q

Decontamination of mycobacterium; trisodium phosphate and benzalkonium chloride are used to clear out what bacteria?

A

Pseudomonas

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16
Q

Decontamination of mycobacterium steps

A

1.Decontaminan
2. Centrifuge decontaminated specimen for 20 mins prior to making smears/inoculating media

17
Q

Types of solutions used to decontaminate mycobacterium

A

1.NALC
2. Trisodium phosphate & benzalkonium chloride
3. Dithiothreitol & 2% NaOH
4. 4% NaOH (strong alkali must be limited to 15 mins May kill mycobacterium)

18
Q

M. Kansasii

A

Nitrate +
Tween 80 <5days
Photo chromogen

19
Q

M. Marinum

A

Tween 80 <5days
Photochromogenic
30*C optimal temp

20
Q

M. Avium

A

Tellurite +
Non photochromogenic

21
Q

M. Fortuitum

A

Rapid grower
Nitrate +
Grows on MAC
Tellurite+

22
Q

M. Chelonae

A

Rapid grower
Grows on MAC

Micro CCMLS (12 decks)

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