GPR

Question 1

catalase + GPR bacteria

Answer 1

Bacillus spp.

Listeria monocytogenes

Question 2

catalase = GPR bacteria

Answer 2

Lactobacillus
Erysipelothrix
Arcanobacterium spp.
Aerobic Actinomycetes (Rhodococcus equi)

Question 3

Bacillus characteristics

Answer 3

found in nature
lab contaminant
spores
large spreading colonies
catalase +
oxidase +
motile

Question 4

key characteristics Bacillus anthracis

Answer 4

non hem
medusa head colony
string of pearls when grown w/ penicillin
non motile
nitrate+
lecithinase +
42*C

Question 5

Bacillus cereus pathogenicity

Answer 5

Chinese restaurant food poisonings
rice
enterotoxin produced
immunocompromised: septicemia, endocarditis, pneumonia, meningitis, opthalmitis, peritonitis

Question 6

key characteristics of Bacillus cereus

Answer 6

beta hem
gray-green colony
motile
lecithinase+

Question 7

Listeria monocytogenes key characteristics

Answer 7

small gray-translucent colonies w/ narrow beta hemolysis
catalase+
vp+
motile @ 25*C tumbling motility
non motile @35*C
bile esculin +
Na hippurate +
\+ CAMP test (shovel)

Question 8

Diptheroids characteristics

Answer 8

normal flora
gpr
palisading, v form, chinese letters
catalase+
oxidase+

Question 9

Corynebacterium diphtheria pathogenicity characteristics

Answer 9

pathogenic strain of diphtheriods
causes pseudomembranous pharyngitis
exotoxin causes necrosis and inflamm.
cellulitis around pseudo-membrance

Question 10

Corynebacterium diphtheriae key characteristics

Answer 10

tinsdale agar: dark brown colonies
ctba: black colonies
catalase+
urease =
motilitly =
nitrate reduction v
acid from glucose+, mannitol+

Question 11

Corynebacterium jeikeium key characteristics

Answer 11

normal skin flora
endocarditis
infection in immunocompromised

Question 12

Corynebacterium urealyticum key characteristics

Answer 12

UTIs
urease= rapid w/in minutes
no glucose ferm.
very resisitant; treat w/ vancomycin

Question 13

Erysipelothrix rhusiopathiae key characteristics

Answer 13

found in pigs
erysipeloid
greenish colony
GPR long thin rods look tangled
non motile
catalase=
h2s +

Question 14

Lactobacillus key characteristics

Answer 14

normal flora in oral, gi, female vaginal tract
pleomorphic GPR
small alpha hem colonies
catalase=
will be confused w/ Streptococci in GS not performed

Question 15

Arcanobacterium haemolyticum key characteristics

Answer 15

small beta colonies
thin gpr in chinese letter pattern
catalase=
reverse CAMP+
pharyngitis in older kids
wound infections, endocarditis, osteomyelitis

Question 16

Aerobic Actinomycetes group bacteria:

Answer 16

Nocardia
Rhodococcus
Actinomadura
Streptomyces
gram positive branching filamentous rods

Question 17

Rhodococcus equi key characteristics

Answer 17

obligate aerobe
partial acid fast
gp bacilli w/ traces of branching
salmon pink colony after 4 days
recovered from sputum, lung biopsy, bronchoalveolar lavage fluid
urease +
\+ camp reverse test
partially acid fast
cause: granulomatous pneumonia w/ lesions in immunocompromised

Question 18

Gardnerella vaginalis key characteristics

Answer 18

clue cells
fishy vaginal odor (whiff test +)
grown on BAP CHOC ppt clear non hem colonies
no growth MAC
metronidazole “s”
V-agar: beta hem.
starch agar: hydrolze starch and turn yellow