OTHER COMMON STAINS

Question 1

– is a mixture of picric acid and fuchsin for the demonstration of connective tissues.

Answer 1

Acid Fuchsin-Picric Acid (Van Gieson’s Stain)

Question 2

– is a basic acridine fluorochrome which permits discrimination between dead and living cells

Answer 2

Acridine Orange

Question 3

– giving fluorescence for DNA and a red fluorescence for RNA.

Answer 3

Acridine Orange

Question 4

– Is used to demonstrate deposits of calcium salts and possible sites of phosphatase activity.

Answer 4

Acridine Red 3B

Question 5

– Is a complex, water-soluble phthalocyanin dye, similar to chlorophyll, which stains acid
mucopolysaccharides by forming salts linkages with them.

Answer 5

Alcian Blue

Question 6

Alcian Blue is a complex, water-soluble phthalocyanin dye, similar to chlorophyll, which stains ________

Answer 6

acid mucopolysaccharides by forming salts linkages with them.

Question 7

– Is a cytoplasmic stain used for counterstaining of epithelial sections.

Answer 7

Aniline Blue

Question 8

– Is a plasma stain utilized also for deep staining of acid-fast organisms, for mitochondria, for
differentiation of smooth muscles with the used of picric acid

Answer 8

Basic Fuchsin

Question 9

– It is main constituents of Feulgen’s (DNA) and Schiff’s reagent for the detection of aldehydes, Van Gieson’s solution for connective tissues, mucin, and for elastic tissue staining.

Answer 9

Basic Fuchsin

Question 10

– Is used as a contrast stain for Gram’s technique, in acid fast and Papanicolau method, and for
staining diphtheria

Answer 10

Bismarck Brown

Question 11

– Is usually combined with aluminum chloride to stain glycogen (Best Carmine Solution)

Answer 11

Carmine

Question 12

– Mayer’s Carmalum Solution

Answer 12

Carmine

Question 13

– Is a mordanted dye acting as a basic dye and staining acidic substances.

Answer 13

Carmine

Question 14

– resistant to strong acid dyes

Answer 14

Celestine Blue

Question 15

– recommended for routine staining of fixed sections, giving a good nuclear definition when used in conjunction with alum hematoxylin.

Answer 15

Celestine blue

Question 16

– Is best known as an indicator, but may be utilized as a stain for axis cylinders embryos.

Answer 16

Congo Red

Question 17

– it is used as a 4% aqueaous solution in Kajian’s method of staining elastic tissues, amyloid and
myelin

Answer 17

Congo red

Question 18

– Is a nuclear or chromatin stain used for staining amyloid in frozen sections and platelets in blood .

Answer 18

Crystal Violet

Question 19

– Crystal Violet + Methyl Violet + Dexterin = Gentian Violet

Answer 19

Crystal violet

Question 20

– is used for staining blood to differentiates leukocytes

Answer 20

Giemsa Stain

Question 21

– Gold sublimate

Answer 21

Giemsa Stain

Question 22

– Is the stained used for metallic impregnation, made up of gold chloride and mercuric chloride

Answer 22

Giemsa stain

Question 23

– It is used for microscopic study of starch granules

Answer 23

Giemsa stain

Question 24

– It is used for removal of mercuric fixative artifact pigments

Answer 24

Giemsa stain

Question 25

– Reagents to alter crystal and methyl violet so that they may retained by certain bacteria and fungi.

Answer 25

Giemsa stain

Question 26

– Is used for demonstrating mitochondria during intravital staining.

Answer 26

Janus Green B

Question 27

– It is used as a contrast stain for staining ascaris eggs and erythrocytes, and as a bacterial spore stain.

Answer 27

Malachite Green

Question 28

– It is also used both as a decolorizer and as a counter stain

Answer 28

Malachite green

Question 29

– Stains chromatin green in the presence of an acid.

Answer 29

Methyl Green

Question 30

– It gives false positive reaction with certain secretions as mucin

Answer 30

Methyl green

Question 31

– is a metachromatic dye formed whenever methylene blue is heated in fixed alkali carbonate

Answer 31

Methylene Violet

Question 32

coloring nuclei of leukocytes reddish-purple in the presence of methylene blue.

Answer 32

Methylene violet

Question 33

– Is a basic dye recommended for observing cell granules and vacuoles of phagocytic cell.

Answer 33

Neutral Red

Question 34

– Used as a substitute for carbol fuchsin in acid-fast staining

Answer 34

Night Blue

Question 35

– Is an excellent stain for elastic fibers (Tanzer Unna Orcein Method)

Answer 35

Orcein

Question 36

– especially recommended in dermatological studies

Answer 36

Orcein

Question 37

– a fixative and it is also used to stain fats

Answer 37

Osmium Tetroxide

Question 38

Fats reduces Osmium tetroxide into_______

Answer 38

Osmium dioxide (Black)

Question 39

– Contrast stain to acid fuchsin

Answer 39

Picric Acid

Question 40

``` – Demonstration of connective tissue – Cytoplasmic stain – Counterstain to violet – Fixative – Decalcifying agent ```

Answer 40

Picric acid

Question 41

- Utilized to manufacture paints

Answer 41

Prussian Blue

Question 42

- Microanatomical color contrast

Answer 42

prussian blue

Question 43

- Demonstration of circulatory system by injection

Answer 43

Prussian blue

Question 44

– Used with osmic acid to fix and stain blood and glandular | – Silver Nitrate

Answer 44

Rhodamine B

Question 45

– Is used in 10% aqueous solution to prepare various dilutions to be used in identification of spirochetes, reticulum and other fiber stains

Answer 45

Rhodamine B

Question 46

– Is a nuclear stain for fixed tissues, used as a substitute for thionine in fresh frozen tissue sections

Answer 46

Toluidine Blue

Question 47

– It is recommended for staining of Nissl granules or chromophile bodies

Answer 47

Toluidine Blue

Question 48

– Is used for demonstration of neuroglia in frozen sections.

Answer 48

Victoria Blue

Question 49

OIL SOLUBLE DYES

Answer 49

LYSOCHROMES

Question 50

LYSOCHROMES

Answer 50

- Sudan Black - Sudan Black B - Sudan IV - Sudan III

Question 51

- most sensitive of the oil soluble dyes

Answer 51

Sudan Black

Question 52

- greater affinity to phospholipids

Answer 52

Sudan Black

Question 53

- stains Phospholipids and neutral fats

Answer 53

Sudan Black B

Question 54

- do not stain crystalline cholesterol and free fatty acids

Answer 54

Sudan Black B

Question 55

- does not color phospholipids, lipid droplets

Answer 55

Sudan IV

Question 56

- recommended for triglycerides

Answer 56

Sudan IV

Question 57

good as a fat stain for CNS tissues

Answer 57

Sudan III