Adhesives

Question 1

• an adhesive is a substance which can be smeared on to the slides so that the sections stick well to the slides. Slides must always be grease- and dust-free and stored and handled correctly.

Answer 1

ADHESIVES

Question 2

are not necessary for routine staining, provided that the slides are clean and free from grease. However, they
are essential for methods that require exposure of sections to acids and alkalis during staining.

Answer 2

ADHESIVES

Question 3

The following are certain instances when sections may float from the slide:

Answer 3

 For urgent cryostat sections
 For CNS tissues
 For tissues containing blood clot
 For tissues that have been decalcified
 When sections are to be subjected to high temperatures

Question 4

• it is the most commonly used because it is very easy to make, is convenient, and is relatively inexpensive.

Answer 4

. Mayer’s Egg Albumin

Question 5

Formula for Mayer’s egg albumin

Answer 5

Egg white 50 cc
Glycerine 50 cc
Thymol 100 mg

Question 6

increases viscosity

Answer 6

Glycerin

Question 7

Preservative; Prevents growth of molds

Answer 7

Thymol

Question 8

– stored in 70% alcohol until it is ready for staining

Answer 8

. Dried Albumin

Question 9

Formula for Dried Albumin

Answer 9

Dried albumin, Sodium chloride, 100 cc Distilled water and Thymol crystals

Question 10

Formula for Gelatin (1%)

Answer 10

Gelatin, Distilled water, Glycerol, Phenol crystal

Question 11

Formula for Gelatin-formaldehyde mixture

Answer 11

1% gelatin, 2% formaldehyde

Question 12

Widely used as a section adhesive in Immunohistochemistry

Answer 12

Poly-L-Lysine

Question 13

They are very useful in cytology, particularly for cytospin preparations of proteinaceous or bloody material.

Answer 13

APES( 3-aminopropylthriethoxysilane)

Question 14

coated slides are better than Poly-Liysine slides because they can be stored for a long time without losing their adhesiveness.

Answer 14

APES