Osmosis & dilution principle Flashcards

1
Q

How does water move across membranes in the bodY?

A

Via aquaporins, protein water channels.

Down a concentration gradient.

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2
Q

Why is it important to remember osmolarity is affected by particles not molecules?

A

Because some solutes dissociate in solution to form multiple particles so the number of molecules isnt always representative of the osmolarity.

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3
Q

What is osmolarity measured in?

A

Osmoles.

This describes the numver of particles/L, e.g. 1M solution of glucose has an osmolarity of 1 osmol/L

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4
Q

What is the osmolarity of normal human plasma?

A

285mOsmol/L (the same as in cells); Usually taken as 300mOsmol/L for ease.

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5
Q

What does a change in concentration of a non-penetrating solute cause?

A

An osmotic flux or immediate net movement of water in one direction r another (changing cell volume)

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6
Q

What are the main non-penetrating solutes in the ECF?

A

Sodium and Potassium

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7
Q

Osmolarity?

A

The number of particles in solution

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8
Q

Tonicity?

A

The number of non-penetrating particles in solution.

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9
Q

What determines cell volume?

A

Tonicity

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10
Q

Whats an isosmotic solution?

A

One with the same total no. solute particles as the ECF

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11
Q

Whats an isotonic solution?

A

One with the same no. non-penetrating solute particles as ECF

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12
Q

Why is the tonicity of normal human ECF close to 285 mOsmol/L?

A

Because most of the solute particles in the ECF are non-penetratin (Na+/Cl- etc)

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13
Q

Why will a RBC in pure water undergo haemolysis?

A
  • High non-penetrating solute numbers in the RBC ICF
  • Water enters cell to try and balance tonicity
  • Water causes RBC to swell & burst.
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14
Q

How is plasma volume (PV) measured using the dilution principle?

A

Plasma proteins cant cross the capillary wall

Therefore dyes or radioactive labels can be attched to plasma proteins.

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15
Q

How is the ECF measured usin the dilution principle?

A

Needs to be able to cross capillary walls but not cell membranes.
e.g. insulin, sucrose

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16
Q

How is TBW measured with the dilution principle?

A

radioactive water (deuterium oxide etc)

17
Q

How is volume of distribution calculateD?

A

Volume of compartment = amount injected(- any extreted or metabolised) divided by sample fluid conc,