Nucleotide Stuff- Watt Flashcards
What is the difference between adenine and guanine?
Adenine has an amino group
What is a nucleoside and a nucleotide?
Nucleoside is base and sugar
Nucleotide is phosphorlyated nucleoside
If you have a five member ring in a synthesis, what are you making?
Purine
If you are building on top of a ribose-P, what are you making?
Purine
If you are utilizing IMP, what are you making?
Purine
If you are using IMP, and you want to make AMP, what is the nitrogen donor?
Aspartate
If you are using IMP and you want to make GMP, what is the nitrogen donor?
Glutamine
If you have no energy, can you continue making adenine and guanine?
No
For the most part, how is the biosynthesis of adenine and guanine regulated?
Negative Feedback
What are the enzymes that are being inhibited by negative feedback?
adenosuccinate synthase - AMP inhibits
IMP dehydrogenase - GMP inhibits
glutamine PRPP aminotransferase- IMP, GMP, AMP inhibit
PRPP synthase- ADP inhibits
If there is a six member ring, and the products are UMP, CTP, and UTP, what is it making?
Pyrimidine
What does ribonuclotide reductase do?
It is a dimer with an active pocket to make CDP and UDP to dTMP
uses radicals
has Fe sites
What does Aspartate Trans Carbamoylase (ATCase) do?
Combines aspartate and carbamoyl-P in pyrimidines synthesis. It is accelerated by ATP and inactivated by its product.
What does tetrahydrofolate (THF) do and why is it significant?
It is a target for cancer treament.
It is used in thymine synthesis
What is an antimetabolite?
Inhibitor of folic acid production
Fluoro uracil and methotrexate are related because….
They inhibit production of folic acid
What is the difference between competitive and suicide inhibitor?
suicide will bind and kills enzyme
competitive will slow down an enzyme
Why are there multiple pathways to ribonucleotide reductase pathways?
so that if one gets shut down then it wont kill you
Where do you excrete uric acid?
kidneys
What is the salvage pathway?
Recycles damaged nucleotides
What is the difference between catabolism of purines and pyrimidines?
purines- breaks down into nitrogen groups for urea cycle, sugar, and base
pyrimidines- breaks down into carbon skeleton (succinyl-CoA) for citric acid cycle; release nitrogens to urea cycle
Lesch-Nyhand Syndrom results from a problem with which degredation pathway?
purine
Orotic Aciduria results from a problem with which degredation pathway?
pyrimidines
