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Bacteriology > Non-fermentative Gram Negative Bacilli > Flashcards

Non-fermentative Gram Negative Bacilli Flashcards

1
Q

Most common non-fermentative organism

A

P. aeroginosa

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2
Q

Characteristic of P. aeroginosa

  • Motility:
  • O2 requirement:
  • Odor:
  • Colony:
  • Pigment:
A
  • Motility: Motile, Single (monotrichous)
  • O2 requirement: Obligate aerobe
  • Odor: Grape-like/Taco/Cornlike/Tortilla
  • Colony: Serrated or Feathery-edge colonies
  • Pigment: Pyocyanin (blue), Pyoverdin (green)
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3
Q

Odor produced by ** P. aeroginosa**

A

Grape-like
Corn Taco like
Tortilla

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4
Q

Non-fluorescent Blue pigment

A

Pyocyanin

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5
Q

Fluoroscent green pigment

A

Pyoverdin

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6
Q

Biochemical Identification of P. aeroginosa

  • IMVC:
  • TSIA:
  • OF test:
  • Citrate:
  • Oxidase:
  • Catalase:
  • Acetamide test:
  • Cetramide test:
  • Gelatin hydriolysis:
  • Growth at 42C:

si KK @ 42 nilabas ang pCOC tas nakipagPAC kaya nilabasan ng GEL

A
  • IMVC: —+
  • TSIA: K/K
  • Glucose OF test (open): Positive
  • Citrate: Positive
  • Oxidase: Positive
  • Catalase: Positive
  • Acetamide test: Positive
  • Cetramide test: Positive
  • Gelatin hydriolysis: Positive
  • Growth at 42C: Positive
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7
Q

Infections caused by P. aeroginosa

A

1 ICU isolate

#1 cause of Cystic Fibrosis
#2 cause of Burn infections
#3 common Nosocomial
Ecthyema gangrenosum
Hot-tub folliculitis
Swimmer’s ear

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8
Q

Burkholderia spp.

A

B. cepacia
B. mallei
B. pseudomallei

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9
Q

Burkholderia spp. that is Non-motile and No growth at 42C

A

Burkholderia mallei

“WALANG (Non-motile, No growth) mallei”

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10
Q

Infection caused by B. cepacia

A

2 Cystic Fibrosis

CGD

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11
Q

Identification B. cepacia

  • Motility:
  • Odor:
  • Glucose:
  • LDC:
  • ONPG:
A
  • Motility: Motile (lopotrichous
  • Odor: Earthy/Dirt-like odor
  • Glucose: Positive
  • LDC: Positive
  • ONPG: Positive
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12
Q

Causative agent of Glander’s disease

A

B. mallei

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13
Q

Identification - B. mallei

  • Motility:
  • Growth at 42C:
  • Glucose:
  • Lactose:
  • Maltose:
  • Nitrate:
  • ADH:
A
  • Motility: Non-motile
  • Growth at 42C: No growth
  • Glucose: Positive
  • Lactose: Positive
  • Maltose: Positive
  • Nitrate: Positive
  • ADH: Positive
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14
Q

Maltose Loving

A

Stenotrophomonas maltophillia

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15
Q

Identification of Stenotrophomonas maltophillia

* Oxidase:
* DNAse:
* Catalase:
* Lysine Decarboxylase:

A
  • Oxidase: Negative
  • DNAse: Positive
  • Catalase: Positive
  • Lysine Decarboxylase: Positive
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16
Q

Infection caused by Burkholderia pseudomallei

A

Malleidosis
Glander’s like disease
Whitmore’s disease

17
Q

Culture medium to grow B. pseudomallei

A

Ashdown medium

18
Q

Odor produced of B. pseudomallei in Ashdown medium

A

Earthy odor

19
Q

Cause the Vietnamese Time Bomb infection

A

Burkholderia pseudomallei

20
Q

Odor produced by A. faecalis

A

Fruity Odor resembling apples or strawberries

21
Q

Alcaligens faecalis characteristics

A

Asaccharolytic
Motile (periotrichous)
Oxidase +

22
Q

Oxidation reaction of A. faecalis in Glucose & Xylose

A

Alkaline in CHO containing media
* Oxidation in Glucose: Alkaline
* Oxidation in Xylose: Alkaline

23
Q

Produces Violet pigment

A

Chromobacterium violaceum

24
Q

Violet pigment produced by Chromobacterium violecium

A

Violacein

25
Q

2 Nonfermentative Organism

A

Acinetobacter baumannii (A. colacoaleticus

26
Q

Characteristics of Acinetobacter spp.

  • Motiltity:
  • MAC:
  • Catalase:
  • Oxidase:
A
  • Motiltity: Non-motile
  • MAC: Positive (grwos well in EMB & MAC)
  • Catalase: Positive
  • Oxidase: Negative
27
Q

Differentiates Acinetobacter baumanni from Acinetobacter lwoffii

A

Rapid assimilation/Fermenter of Glucose and Lactose

28
Q

Agent of Blepharo conjunctivitis

A

Moraxella lacunata

29
Q

Associated to formation of yellow milk

A

Flavobacterium spp.

30
Q

Violacein produced by Chromobacterium violaceum is soluble in ____________ but incoluble in ___________

A

Violacein produced by Chromobacterium violaceum is soluble in ETHANOL but insoluble in WATER

Bacteriology (24 decks)

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