Non-fermentative Gram Negative Bacilli Flashcards
Most common non-fermentative organism
P. aeroginosa
Characteristic of P. aeroginosa
- Motility:
- O2 requirement:
- Odor:
- Colony:
- Pigment:
- Motility: Motile, Single (monotrichous)
- O2 requirement: Obligate aerobe
- Odor: Grape-like/Taco/Cornlike/Tortilla
- Colony: Serrated or Feathery-edge colonies
- Pigment: Pyocyanin (blue), Pyoverdin (green)
Odor produced by ** P. aeroginosa**
Grape-like
Corn Taco like
Tortilla
Non-fluorescent Blue pigment
Pyocyanin
Fluoroscent green pigment
Pyoverdin
Biochemical Identification of P. aeroginosa
- IMVC:
- TSIA:
- OF test:
- Citrate:
- Oxidase:
- Catalase:
- Acetamide test:
- Cetramide test:
- Gelatin hydriolysis:
- Growth at 42C:
si KK @ 42 nilabas ang pCOC tas nakipagPAC kaya nilabasan ng GEL
- IMVC: —+
- TSIA: K/K
- Glucose OF test (open): Positive
- Citrate: Positive
- Oxidase: Positive
- Catalase: Positive
- Acetamide test: Positive
- Cetramide test: Positive
- Gelatin hydriolysis: Positive
- Growth at 42C: Positive
Infections caused by P. aeroginosa
1 ICU isolate
#1 cause of Cystic Fibrosis
#2 cause of Burn infections
#3 common Nosocomial
Ecthyema gangrenosum
Hot-tub folliculitis
Swimmer’s ear
Burkholderia spp.
B. cepacia
B. mallei
B. pseudomallei
Burkholderia spp. that is Non-motile and No growth at 42C
Burkholderia mallei
“WALANG (Non-motile, No growth) mallei”
Infection caused by B. cepacia
2 Cystic Fibrosis
CGD
Identification B. cepacia
- Motility:
- Odor:
- Glucose:
- LDC:
- ONPG:
- Motility: Motile (lopotrichous
- Odor: Earthy/Dirt-like odor
- Glucose: Positive
- LDC: Positive
- ONPG: Positive
Causative agent of Glander’s disease
B. mallei
Identification - B. mallei
- Motility:
- Growth at 42C:
- Glucose:
- Lactose:
- Maltose:
- Nitrate:
- ADH:
- Motility: Non-motile
- Growth at 42C: No growth
- Glucose: Positive
- Lactose: Positive
- Maltose: Positive
- Nitrate: Positive
- ADH: Positive
Maltose Loving
Stenotrophomonas maltophillia
Identification of Stenotrophomonas maltophillia
* Oxidase:
* DNAse:
* Catalase:
* Lysine Decarboxylase:
- Oxidase: Negative
- DNAse: Positive
- Catalase: Positive
- Lysine Decarboxylase: Positive
Infection caused by Burkholderia pseudomallei
Malleidosis
Glander’s like disease
Whitmore’s disease
Culture medium to grow B. pseudomallei
Ashdown medium
Odor produced of B. pseudomallei in Ashdown medium
Earthy odor
Cause the Vietnamese Time Bomb infection
Burkholderia pseudomallei
Odor produced by A. faecalis
Fruity Odor resembling apples or strawberries
Alcaligens faecalis characteristics
Asaccharolytic
Motile (periotrichous)
Oxidase +
Oxidation reaction of A. faecalis in Glucose & Xylose
Alkaline in CHO containing media
* Oxidation in Glucose: Alkaline
* Oxidation in Xylose: Alkaline
Produces Violet pigment
Chromobacterium violaceum
Violet pigment produced by Chromobacterium violecium
Violacein
2 Nonfermentative Organism
Acinetobacter baumannii (A. colacoaleticus
Characteristics of Acinetobacter spp.
- Motiltity:
- MAC:
- Catalase:
- Oxidase:
- Motiltity: Non-motile
- MAC: Positive (grwos well in EMB & MAC)
- Catalase: Positive
- Oxidase: Negative
Differentiates Acinetobacter baumanni from Acinetobacter lwoffii
Rapid assimilation/Fermenter of Glucose and Lactose
Agent of Blepharo conjunctivitis
Moraxella lacunata
Associated to formation of yellow milk
Flavobacterium spp.
Violacein produced by Chromobacterium violaceum is soluble in ____________ but incoluble in ___________
Violacein produced by Chromobacterium violaceum is soluble in ETHANOL but insoluble in WATER
