Anaerobic Microorganisms Flashcards
Anaerobic Organisms
mnemonics: Can’t Breath Fresh Air
Chlostridia
Bacteriodes
Fusobacterium
Actinomyces
General Characteristics of anaerobic organisms
Produces Foul Odor
Polymicrobic infections
Gas in tissues
Specimen of Choice
Needle aspirate
Anaerobic incubation requirement
5% O2
10% CO2
85% Nitrogen
Indicator of Anaerobic effectiveness
Methylene blue
Resazurin
Methylene Blue Indicator
Interpret:
Blue
Colorless
Interpret:
Blue - Presence of O2
Colorless - Absence of O2
Toxin produced by C. tetani
Tetanospasmin
Infection caused by C. tetani
Tetanus - Spastic Paralysis
Classic Symptoms of Tetanus
Trismus (lock jaw)
Risus sarcodinicus (Sarcodonic smile)
Opisthotonus
Differntial Identification of C. tetani
- Motility:
- Lecithinase:
- Lipase:
- Glucose:
- Lactose:
- Motility: Motile
- Lecithinase: Negative
- Lipase: Negative
- Glucose: Negative
- Lactose: Negative
Microscopic appearance of C. tetani
Lollipop/Drumstick appearance (terminal spores)
Infection caused by C. botulinum
Botulism - Flaccid Paralysis
Toxin produced by C. botulinum
Botulinum toxin
Types of Botulism
- Food botulism:
- Infant botulism
- Wound botulism:
Types of Botulism
* Food botulism: Ingestion of preformed toxin in contaminated FOOD
* Infant botulism: Ingestion of spores in contaminated HONEY
* Wound botulism: growth after infection with botulism
Flappy baby
Infant botulism from ingestion of spores
Canned good bacillus
C. botulinum
Microscopic appearance of C. botulinum
Tennis-rocket appearance (sub-terminal spores)
Box-car shaped bacillus
C. perfringens
Infection caused by C. perfringens
Gas Gangrene
Food Poisoning
Toxins produced by C. perfringens
Alpha toxin
Enterotoxin
Causes Double Zone of Hemolysis
C. perfringens
Double Zone of Hemolysis
- Inner zone:
- Outer zone:
- Inner: Beta hemolysis
- Outer: Alpha hemolysis
Differential identification of C. perfringens
- Motility:
- Lecithinase:
- Lipase:
- Glucose:
- Lactose:
- Motility: Nonmotile
- Lecithinase: Positive
- Lipase: Negative
- Glucose: Positive
- Lactose: Positive
Streaked organism in Reverse CAMP test
S. agalactiae
Lecithinase test or Nagler’s reaction
Identify organisms that liberate phospholipases (lecithinases) inhibited by anti-perfringens (Type A antitoxin)
Toxin produced by C. difficle causing pseudomembranous colitis
Toxin B - Cytotoxin
Infection caused by C. difficile
Pseudomembranous colitis in patien with antibiotic therapy (clindamycin)
identify
Licethenase Negative
Lipase Positive
C. botulinum
Colony morphology of C. difficile in CCFA
Yellow , Ground glass-colonies
Grow Best in 20% Bile and Hydorlyzes esculin
Bacteriodes fragilis
Most common anerobe isolated from infectious processes of soft tissues and anaerobic bacteremia
B. fragilis
Anaerobic gram negative rod with tapered ends
Fusobacterium nucleatum
Bread-crumbs colonies
Fusobacterium nucleatum
Anaerobic fungi-like organism
Actinomyces spp.
Molartooth colonies
Actinomyces israelli
Commonly associated skin contaminant in blood cultures
Propionobacterium acnes
Characterized by SULFUR granules
A. israelii
Anaeorobic staphylopcoccus
Peptococcus
Anaerobic streptococcus
Peptostreptococcus
Inhibited by SPS
Peptostreptococcus anaerobius
Produces Brick-red fluorescence in UV
mnemonics: PPV
Provetella
Porphyromonas
Villionella
Infection caused by Actinomyces israelii
Lumpy jaw
