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Bacteriology > Anaerobic Microorganisms > Flashcards

Anaerobic Microorganisms Flashcards

1
Q

Anaerobic Organisms

mnemonics: Can’t Breath Fresh Air

A

Chlostridia
Bacteriodes
Fusobacterium
Actinomyces

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2
Q

General Characteristics of anaerobic organisms

A

Produces Foul Odor
Polymicrobic infections
Gas in tissues

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3
Q

Specimen of Choice

A

Needle aspirate

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4
Q

Anaerobic incubation requirement

A

5% O2
10% CO2
85% Nitrogen

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5
Q

Indicator of Anaerobic effectiveness

A

Methylene blue
Resazurin

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6
Q

Methylene Blue Indicator

Interpret:
Blue
Colorless

A

Interpret:
Blue - Presence of O2
Colorless - Absence of O2

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7
Q

Toxin produced by C. tetani

A

Tetanospasmin

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8
Q

Infection caused by C. tetani

A

Tetanus - Spastic Paralysis

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9
Q

Classic Symptoms of Tetanus

A

Trismus (lock jaw)
Risus sarcodinicus (Sarcodonic smile)
Opisthotonus

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10
Q

Differntial Identification of C. tetani

  • Motility:
  • Lecithinase:
  • Lipase:
  • Glucose:
  • Lactose:
A
  • Motility: Motile
  • Lecithinase: Negative
  • Lipase: Negative
  • Glucose: Negative
  • Lactose: Negative
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11
Q

Microscopic appearance of C. tetani

A

Lollipop/Drumstick appearance (terminal spores)

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12
Q

Infection caused by C. botulinum

A

Botulism - Flaccid Paralysis

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13
Q

Toxin produced by C. botulinum

A

Botulinum toxin

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14
Q

Types of Botulism

  • Food botulism:
  • Infant botulism
  • Wound botulism:
A

Types of Botulism
* Food botulism: Ingestion of preformed toxin in contaminated FOOD
* Infant botulism: Ingestion of spores in contaminated HONEY
* Wound botulism: growth after infection with botulism

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15
Q

Flappy baby

A

Infant botulism from ingestion of spores

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16
Q

Canned good bacillus

A

C. botulinum

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17
Q

Microscopic appearance of C. botulinum

A

Tennis-rocket appearance (sub-terminal spores)

18
Q

Box-car shaped bacillus

A

C. perfringens

19
Q

Infection caused by C. perfringens

A

Gas Gangrene
Food Poisoning

20
Q

Toxins produced by C. perfringens

A

Alpha toxin
Enterotoxin

21
Q

Causes Double Zone of Hemolysis

A

C. perfringens

22
Q

Double Zone of Hemolysis

  • Inner zone:
  • Outer zone:
A
  • Inner: Beta hemolysis
  • Outer: Alpha hemolysis
23
Q

Differential identification of C. perfringens

  • Motility:
  • Lecithinase:
  • Lipase:
  • Glucose:
  • Lactose:
A
  • Motility: Nonmotile
  • Lecithinase: Positive
  • Lipase: Negative
  • Glucose: Positive
  • Lactose: Positive
24
Q

Streaked organism in Reverse CAMP test

A

S. agalactiae

25
Lecithinase test or Nagler's reaction
Identify organisms that liberate phospholipases (lecithinases) inhibited by **anti-perfringens (Type A antitoxin)**
26
Toxin produced by C. difficle causing **pseudomembranous colitis**
Toxin B - Cytotoxin
27
Infection caused by C. difficile
Pseudomembranous colitis in patien with antibiotic therapy **(clindamycin)**
28
# identify Licethenase Negative Lipase Positive
C. botulinum
29
Colony morphology of C. difficile in CCFA
Yellow , Ground glass-colonies
30
Grow Best in **20% Bile** and Hydorlyzes esculin
Bacteriodes fragilis
31
Most common anerobe isolated from **infectious processes** of soft tissues and **anaerobic bacteremia**
B. fragilis
32
Anaerobic gram negative rod with **tapered ends**
Fusobacterium nucleatum
33
Bread-crumbs colonies
Fusobacterium nucleatum
34
Anaerobic fungi-like organism
Actinomyces spp.
35
Molartooth colonies
Actinomyces israelli
36
Commonly associated **skin contaminant in blood cultures**
Propionobacterium acnes
37
Characterized by **SULFUR** granules
A. israelii
38
Anaeorobic staphylopcoccus
Peptococcus
39
Anaerobic streptococcus
Peptostreptococcus
40
Inhibited by SPS
Peptostreptococcus anaerobius
41
Produces Brick-red fluorescence in UV | mnemonics: PPV
Provetella Porphyromonas Villionella
42
Infection caused by *Actinomyces israelii*
Lumpy jaw

Bacteriology (24 decks)

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