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Biochemical Tests Flashcards

1
Q

Microdase reagent

A

Tetramethly paraphenylene diamine dihydrochloride

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2
Q

Positive Result in Microdase Test

A

Dark blue

(+) Micrococci

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3
Q

Catalase test reagent

A

3% H2O2

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4
Q

Positive result in Catalase Test

A

Gas formation
Bubbles
Effervescence

(+) Staphylococcus, Micrococcus (-) Streptococcus

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5
Q

Organism detected by Superoxol test

A

Neiserria gonorrhea

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6
Q

Superoxol reagent

A

30% H2O2

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7
Q

Detected in Slide coagulase tets

A

Bound coagulase “Clumping factor”

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8
Q

Detected in Tube coagulase test

A

Free coagulase “Coagulase reacting factor”

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9
Q

If slide coagulase is negative, what test is followed?

A

Tube test

rationale: negative slide must be confirmed with tube test

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10
Q

If tube test is negative, what shoul you do next?

A

Reincubate at RT from 18-24 hrs

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11
Q

Coagulase test reagent

A

EDTA rabbit plasma

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12
Q

Test that differentiate S, aureus from other Staphylococci

A

Coagulase test

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13
Q

Test that differentiate Staphylococci, Micrococci from Streptococci

A

Catalase test

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14
Q

Test that differentiate coagulase negative staphylococci

A

Novobiocin susceptibility test

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15
Q

Positive result of Novobiocin

A

Zone of inhibition > 16 mm

Susceptible - S. epidermidis; Resistant - S. saprophyticus

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16
Q

Positive Result in DNA Hydrolysis

A

Colorless/Clear zone

(+) MASSS: Moraxella, Aeromonas, S. aureus, Serratia, Stenotrophomonas

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17
Q

Positive for PYR Test

A

S. pyogenes
E. faecalis (Enterococci)

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18
Q

Positive result of PYR test

A

Bright Red or Pink-to-Cheryy Red (De lost)

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19
Q

Bacitracin Disk and Units

A

Taxo A with 0.04 units

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20
Q

Differentiate Group A from Group B

A

Bacitracin test

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21
Q

Positive result of Bacitracin test

A

Zone of inhibition >10 mm

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22
Q

End product of Hippurate hydrolysis

A

Glycine and Benzoic acid

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23
Q

Positive result of Hippurate hydrolysis

A

Deep purple

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24
Q

Reagent of SXT susceptibility test

A

1.25 ug

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25
Differemtiate S. agalactiae from S. pyogenes
CAMP Test
26
Organism that its helolysis is enhanced by CAMP factor of S. agalactiae
S. aureus
27
Positive result in CAMP test
Enhanced hemolysis with **Arrowhead zone of beta hemolysis**
28
Negative test in CAMP test
No enahancement of hemolysis
29
Test used to identify S. agalactiae
Hippurate hydroilysis CAMP test
30
Causes False positive CAMP test
Incubated **anaerobically** or in **capnophilic** environment | CAMP test-plate must be placed in an ambient air incubator at 35C to 37C
31
Meaning of **Quellung**
Swelling
32
Positive test of Neufeld quellung test
Capsulaw swelling | (+) S. pneumoniae, N. meninigitidis
33
Taxo P disk
Optochin test - ethyl hydrocupreine hydrochloride
34
Taxo P disk concentration
0.5 ug/ml
35
Positive Optochin susceptibility test
Zone of inhibition of **>14 mm**
36
**Confirms for S. pneumoniae** after optochin test
Bile solubility test
37
Positive Bile solubility test
Lysis of colonies | (+) S. pneumoniae
38
Differentiate Group D (enterococci & non-enterococci) from Viridans group
Bile esculin test
39
Positive Bile esculine Test
Brown-Black precipitate
40
Differentiate **Enterococci from Non-enetrococci**
6.5% NaCl PYR test
41
Positive 6.5% NaCl test result
Visible turbidity | (+) E. faecalis, E. faecium (-) S. bovis
42
Differentiate **E. faecalis from E. faecium**
Pyruvate Broth | (+) E. faecalis (-) E. faecium
43
Positive Pyruvate broth test result
Change in color from **green-yellow**
44
Differentiate **E. faecalis from Leuconostoc**
LAP test | (+) E. faecalis; (-) Leuconostoc
45
Determine gas production during lactose fermentation esp. of **Leuconostoc from Pediococcus sp.**
MRS broth | (+) Leconostoc sp. (-) Pediococcus
46
Differentiate **Micrococci from Staphylococci**
Microdase test
47
Test that detets cytochrome c oxidase
Oxidase test
48
Oxidase Test reagent
tetramethyl-p-phenylenediamine dihydrochloride
49
Positive oxidase test result
dark purple
50
Ways to perform oxidase test
1. Rub a colony on a paper strip and add reagenr; or 2. Rub the colony om a piece of paper containing the reagent; or 3. Put a drop of reagent on the colony
51
Used to **remove colony** in Oxidase Test
Platinum looop or wooden applicator | DO NOT USE NICHROME LOOP - it contains IRON which can give FP result
52
Acetamide Incubation
35C for 7 days
53
Positive Acetamide Result
Royal Blue
54
Blue Color as Positive Results | Mnemonics: MAAC
Malonate Utilization test Acetamide test Acetate Utilization test Citrate utilization test
55
Indicator in Citrate utilization test
Bromthymol blue
56
Causes false positive in Citrate
Inoculum is heavy
57
Hydrolyzed to form Indole
Trytophan | Tryptophase splits tryptophane to form indole
58
Kova'c/Ehrlich reagent
p-dimethylaminobenzaldehyde
59
**More preffered** reagent in Indole test
Ehrlich's method | It is more sensitive for detecting small amounts of indole
60
Ehrlich's indole test **uses extraction** from broth using __________.
Ehrlich's indole test uses extraction from broth using **XYLENE**.
61
Positive Indole Test
Red ring (Pink-to-wine colored ring)
62
Methyl Red Fermentation
**Mixed acid production** - stable acid end product
63
Methyl Red Pathway
Acidic Pathway
64
Methyl red is RED at what pH?
**4.5**
65
Positive MR result
Bright Red
66
Voges Proskauer (VP) Fermentation
Acetoin Fermentation - neutral acid end products
67
VP end products
Acetoin/Acetyl methyl carbinol 2,3-butanediol
68
VP Pathway
Butylene glycol pathway
69
VP reagent
1st: a-naphthol 2nd: 40% KOH/NaOH
70
Positive VP result
Red
71
MUG Test Result: * Positive: * Negative:
MUG Test Result: * Positive: Electric blue fluorescence * Negative: lack of fluorescnce
72
Next step when no color develops (negative) in nitrate reduction?
Add **Zinc dust**
73
Use of addition of **Zinc dust** in Nitrate reduction test
Reduces nitrate to nitrite and differentiate FN and TN result.
74
Positive Result after addition of Zinc dust
No color | Therefore, FN and indicates that bacteria reduced nitrate NH3, NO2, N2O2
75
Negative Nitrate rediuction after addition of Zinc dust
Red Color
76
Test for late lactose fermenter
ONPG test
77
Enzyme that is only produced by late lactose fermenters
Beta galactosidase
78
An enzyme that change ONPG to orthonitrophenol
Beta galactosidase
79
Positive ONPG Test
Yellow
80
Biochemical medium for H2S Production
TSIA LIA SIM
81
TSIA Carbohydrate Composition
Glucose - 0.1% Sucrose - 1% Lactose - 1%
82
Test that differentiate Enterobacteriaciaeae
TSIA
83
KIA Carbohydrate Composition
Glucose Lactose | KIA is same with TSIA **except NO SUCROSE**
84
Urease Tes Medium
Christensen Urea Agar
85
Indicator in Urease Test
Phenol Red
86
Products from Hydrolysis if Urea
Ammonia and CO2
87
Positive Urease Test
Magenta or Pink (pH - 8.1)
88
Positive PAD Test
Green Color
89
Moeller's Method
Decarbocylase Test
90
Decarboxylation of Lysine
**CADAVERINE**
91
Deacrboxylation of Ornithine & Arginine
Putrescine
92
Arginine is hydrolyzed into ____________ and then into ____________ which is converted into __________.
Arginine is hydrolyzed into **cetrulline** and then into **ornithine** which is converted into **putrescine**.
93
Differentiate Klebsiella from Enterobacter
Motility Arginine Decarboxylation | Klebsiella - NM, Negative; Enterobacter - Mitile, Positive
94
Test that differentiates Enterobacter spp.
Decarbocylase test
95
Test that differentiates E. tarda from E. coli
H2S Lactose
96
Test to differentiate Citrobacter from salmonella
ONPG Lysine decarboxylase

Bacteriology (24 decks)

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