Bacterial Identification Flashcards

1
Q

Staphylococcos aureus Identification

  • Arrangement:
  • O2 Requirement:
  • Hemolysis:
  • Glucose utilization:
  • Bacitracin:
  • Furazolidone:
  • Lysostaphin
  • Microdase:
  • Catalase:
  • Coagulase:
  • DNAse:
  • Colonies:
A
  • Arrangement: Grape-like in clusters
  • O2 Requirement: Facultative anaerobe
  • Hemolysis: Beta hemolysis
  • Glucose utilization: Fermentation
  • Bacitracin: Resistant
  • Furazolidone: Susceptible
  • Lysostaphin: Susceptible
  • Microdase: Negative
  • Catalase: Positive
  • Coagulase: Positive
  • DNAse: Positive
  • Colonies: Golden yellow colonies
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2
Q

Micrococci Identification

  • Arrangement:
  • O2 Requirement:
  • Hemolysis:
  • Glucose utilization:
  • Bacitracin:
  • Furazolidone:
  • Lysostaphin
  • Microdase:
  • Catalase:
  • Coagulase:
A
  • Arrangement: Tetrads/Packets
  • O2 Requirement: Obligate aerobe
  • Hemolysis: Non-hemolytic
  • Glucose utilization: Oxidation (open tube +)
  • Bacitracin: Susceptible
  • Furazolidone: Resistant
  • Lysostaphin: Restant
  • Microdase: Positive
  • Catalase: Positive
  • Coagulase: Negative
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3
Q

S. epidermidis Identification

  • Catalase:
  • Coagulase:
  • Novobiocin:
  • BAP:
A
  • Catalase: Positive
  • Coagulase: Negative
  • Novobiocin: Susceptible (>16 mm)
  • BAP: Sticky white
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4
Q

S. saprophyticus Identification

  • Catalase:
  • Coagulase:
  • Novobiocin:
  • BAP:
A
  • Catalase: Positive
  • Coagulase: Negative
  • Novobiocin: Resistant (<16 mm)
  • BAP: Large yellow/orange/white
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5
Q

Identification of Group A - S. pyogenes

  • Bacitracin:
  • SXT:
  • PYR Test:
  • CAMP Test:
  • Hippurate Test:
A
  • Bacitracin: Susceptible
  • SXT: Resistant
  • PYR Test: Positive
  • CAMP Test: No enhanced hemolysis
  • Hippurate Test: Negative
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6
Q

Identification of Group B - S. agalactiae

  • Bacitracin:
  • SXT:
  • PYR Test:
  • CAMP Test:
  • Hippurate Test:
  • Bile Esculin:
  • Growth in 6.5%:
A
  • Bacitracin: Resistant
  • SXT: Resistant
  • PYR Test: Negative
  • CAMP Test: Arrowhead hemolysis
  • Hippurate Test: Positive
  • Bile Esculin: Negative
  • Growth in 6.5%: Positive
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7
Q

Identification of S. pneumoniae

  • Optochin:
  • Bile Solubility:
  • Inulin Fermentation:
  • Animal inoculation:
  • Neufeld quellung’s test:
A
  • Optochin: Susceptible
  • Bile Solubility: Soluble
  • Inulin Fermentation: Positive
  • Animal inoculation: Positive
  • Neufeld quellung’s test: Capsulaw swelling
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8
Q

Identification of Viridans Streptococci

  • Optochin:
  • Bile Solubility:
  • Growth in 6.5% NaCl:
  • Bile esculin:
  • Inulin Fermentation:
  • Animal inoculation:
  • Neufeld quellung’s test:
A
  • Optochin: Resistant
  • Bile Solubility: Insoluble
  • Growth in 6.5% NaCl: Positive
  • Bile esculin: Negative
  • Inulin Fermentation: Negative
  • Animal inoculation: Negative
  • Neufeld quellung’s test: Negative
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9
Q

Identification of E. coli

  • MAC:
  • EMB:
  • TSIA:
  • IMVC:
  • LOA:
  • Motility:
  • H2S:
  • Urease:
  • PAD:
A
  • MAC: flat, dry with sorrounding dark pink area precipitated with bile salts
  • EMB: Green metallic sheen
  • TSIA: A/A
  • IMVC: ++00
  • LOA: ++0
  • Motility: Positive
  • H2S: Negative
  • Urease: Negative
  • PAD: Negative
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10
Q

Identification of K. pneumoniae

  • MAC:
  • TSIA:
  • IMVC:
  • Motility:
  • LOA:
  • H2S:
  • PAD:
  • Urease:
A
  • MAC: Mucoid colonies
  • TSIA: A/A
  • IMVC: –++
  • Motility: Nonmotile
  • LOA: +00
  • H2S: Negative
  • PAD: Negative
  • Urease: Positive
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11
Q

Identification of K. oxytoca

  • MAC:
  • TSIA:
  • IMVC:
  • Motility:
  • LOA:
  • H2S:
  • PAD:
  • Urease:
A
  • MAC: Mucoid colonies
  • TSIA: A/A
  • IMVC: +-++
  • Motility: Nonmotile
  • LOA: +00
  • H2S: Negative
  • PAD: Negative
  • Urease: Positive
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